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SO7 gene of Eizneria tenella YZ strain was amplified by RT-PCR from total RNA of sporulating oocyst, cloned into pUCm-T, and then sequenced.

通过RT—PCR扩增柔嫩艾美球虫YZ株折光体SO7抗原基因，并进行克隆和测序。

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

Accordingly, the more complete mitochondrial genome sequences of Orthoptera species are desirable to be sequenced and analyzed.

分析了它们线粒体基因组的基因和蛋白质组成、同义密码子差异、tRNA和rRNA结构等多层次信息，并联合GenBank收录的六足总纲全线粒体基因组数据对系统发育关系进行重建。

Until now, it was the first time that the ouabain ScFv was cloned and sequenced by us in the world.

至此，我们在国际上首次克隆并测序了哇巴因单链抗体基因。

One positive clone that can integrate specifically with ouabain with higher affnity was sequenced.

选取一个与特异性抗原结合活性比较高的噬菌体抗体阳性克隆，将其V区基因克隆后进行序列分析。

Total RNA was isolated from cultured human pigmental cells and mRNA was reversly transcribed into cDNA. Then PCR was used to amplify the TRP -2 coding region. The PCR product was cloned into pUC19 plasmid and sequenced, then subcloned into vector pGEX-4T-1. The TRP-2 protein was expressed hi E coli of DH5 a as fusion protein with glutathioneS-transferase induced by IPTG. The fusion protein was purified by glutathione resin column.

我们通过RT—PCR的方法扩增TRP—2编码基因，克隆至pUC19载体并测序，再亚克隆至GST融合表达载体pGEX—4T—1，转化大肠杆菌，IPTG诱导表达了GST/TRP—2融合蛋白，并分析鉴定表达蛋白的可溶性，最后第四军医大学硕士学位论文一用谷氨酚胺树脂柱纯化表达的蛋白。

In this study, the full-length hsp70 cDNA was firstly isolated and sequenced from planarian Dugesia japonica (Djhsp70) using rapid amplification of cDNA end technology.

研究采用 RACE (Rapid amplification of cDNA end)技术首次从日本三角涡虫中克隆出hsp70 (Djhsp70)全长cDNA序列。

Since the human genome was sequenced six years ago, the cost of producing a high-quality genome sequence has dropped precipitously.

从六年前人类基因组测序的完成，高品质的基因组测序结果的花费已经明显降低。

90In this paper, the 10kDa sulfur-rich prolamin gene was amplified by PCR, cloned and sequenced from Chi-nese rice. The sequence analysis showed that the amplified fragment was 380bp long, 94. 5 % homologousto the nucleic acid sequence reported by Masumura et al.

对扩增产物的核苷酸序列分析表明：本扩增产物长 380bp；与 Masumura等人[1] 发表的序列相比，有94.5％的同源性，与我们以前发表的中花10号水稻10kDa富硫醇溶蛋白基因的序列相比，有99.5％的同源性。

The PCR product was cloned into pCR BluntⅡ-TOPO vector to form subclone and then sequenced.The subclone product were cloned into the improved pBluescript Ⅱ KS vector in proper order by suitable enzyme sites to obtain viral full length genomic cDNA clone.

根据测序结果，选择合适的酶切位点将各个亚克隆产物逐段克隆入改造过的pBluescript Ⅱ KS载体中，从而获得了病毒的全长基因组cDNA克隆。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。