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The ribosomal 16s rDNA of these two strains were amplified and the PCR products were sequenced.

对含有这两个菌株成分的混合生防菌剂GJ23进行对根结线虫的离体生物测定。

Methods: Oocytes in the GV stage were separated from ovary by squeezing method. In mouse germinal vesicle GV stage, the expression of ATP8 gene in the mitochondria in the single oocyte was detected by RT-PCR, in which, cDNA was synthesized with two methods: one was the single GV-stage oocyte directly to be placed RT, the other was to perform RT after eliminating mtDNA and nucleus DNA with the EeoR Ⅰ enzyme and Dnase. And the product of RT-PCR was cloned and sequenced.

应用挤压法从卵巢中分离获得生发泡期(germinal vesicle， GV)卵母细胞；用RT-PCR检测GV期单个卵母细胞中ATP8基因的表达：其中cDNA的合成分两种方法进行：一是将GV期单个卵母细胞直接进行RT合成cDNA，二是先用DNA酶加EcoR Ⅰ酶祛除mtDNA和核DNA后再进行RT；回收产物构建克隆质粒并测序。

The PCR technique was used to amplify the mtDNA D-Loop in 20 individuals of Spanish mackerel collected from the southern coast waters of the Shandong Peninsula. The PCR products were accumulated, purified and sequenced.

采用聚合酶链式反应技术对采自山东半岛南岸水域的蓝点马鲛20个个体的mtDNA D-Loop基因进行扩增，得到了1条大小约为500bp的清晰带。

HCV E 2 region was amplified by RT-PCR with type-specific primers targeted on E 2 region,and then sequenced.

多变性是丙型肝炎病毒的主要特点，在HCV基因区段中膜区(E2 )是变化最大的区段〔1〕。

The main functional region of the F gene including 535 nucleotides was amplified by RT-PCR and sequenced.

运用RT—PCR扩增了该分离株F基因的重要功能片段（约535kb），并进行了序列测定。

"The operators will suffer a temporary decrease in the number of consumers and short message services as people may not like having to provide their ID cards for a phone number," Xu Junqi, vice-dean with the Policy-Making Institute of the Telecommunications Research Centre under MII, said yesterday.

信息产业部下属的通信决策研究院副主任徐俊其昨天说：&实行手机实名制后，人们可能不太情愿用身份证注册手机号，所以通信运营商会暂时面临用户减少和短信用户减少的问题。&

I don't believe in make-believe!

我不相信这些虚构的故事。