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The ribosomal 16s rDNA of these two strains were amplified and the PCR products were sequenced.

对含有这两个菌株成分的混合生防菌剂GJ23进行对根结线虫的离体生物测定。

Methods: Oocytes in the GV stage were separated from ovary by squeezing method. In mouse germinal vesicle GV stage, the expression of ATP8 gene in the mitochondria in the single oocyte was detected by RT-PCR, in which, cDNA was synthesized with two methods: one was the single GV-stage oocyte directly to be placed RT, the other was to perform RT after eliminating mtDNA and nucleus DNA with the EeoR Ⅰ enzyme and Dnase. And the product of RT-PCR was cloned and sequenced.

应用挤压法从卵巢中分离获得生发泡期(germinal vesicle， GV)卵母细胞；用RT-PCR检测GV期单个卵母细胞中ATP8基因的表达：其中cDNA的合成分两种方法进行：一是将GV期单个卵母细胞直接进行RT合成cDNA，二是先用DNA酶加EcoR Ⅰ酶祛除mtDNA和核DNA后再进行RT；回收产物构建克隆质粒并测序。

The PCR technique was used to amplify the mtDNA D-Loop in 20 individuals of Spanish mackerel collected from the southern coast waters of the Shandong Peninsula. The PCR products were accumulated, purified and sequenced.

采用聚合酶链式反应技术对采自山东半岛南岸水域的蓝点马鲛20个个体的mtDNA D-Loop基因进行扩增，得到了1条大小约为500bp的清晰带。

HCV E 2 region was amplified by RT-PCR with type-specific primers targeted on E 2 region,and then sequenced.

多变性是丙型肝炎病毒的主要特点，在HCV基因区段中膜区(E2 )是变化最大的区段〔1〕。

The main functional region of the F gene including 535 nucleotides was amplified by RT-PCR and sequenced.

运用RT—PCR扩增了该分离株F基因的重要功能片段（约535kb），并进行了序列测定。

For the head-teacher of the class said that I had seriously broken the school rules,which led me to a three-day suspend class.

为什么呢？因为我的班主任说我严重的违反了校规，于是让我停课三天。

Many of them believe that the conversion of thousands of working-class folk in England spared that nation from the mass carnage and the tyranny that came with the revolution in France.

他们之中有许多人相信，在英国数以千计的劳工阶级之悔改信主使英国免於遭受如法国大革命所造成的大屠杀和专制暴政。