查询词典 sequenced

In our study, the omp1 gene of Chlamydophila abortus of swine was amplified by PCR and was sequenced.

本研究通过PCR扩增了猪流产嗜性衣原体omp1基因，并进行了测序鉴定。

The Omp25 gene of wapiti's Brucella abortus was amplified by RT-PCR and sequenced, Then the interesting gene was subcloned into pET28a for expression. The results showed that the full length of Omp25 gene was 642bp, which encoded 213 amino acids.

运用RT-PCR技术从马鹿布氏杆菌分离株中扩增出Omp25基因，将其克隆入pMD18-T中进行核苷酸序列测定和遗传变异分析，并将目的基因亚克隆到大肠杆菌表达载体pET28a中诱导表达。

Japonicus and its 3 adulterant species were amplified and sequenced, and CLUSTRAL X and MEGA software was employed for analysis.

对4种益母草属植物分别进行ITS序列的PCR扩增和测序，并运用CLUSTRALX和MEGA软件进行分析。

Aiming at the two major migrating routs of wild birds in China, the ecological distribution and prevalence of avian influenza were studied based on the investigation and analysis of the occurring and relative influential factors of highly pathogenic avian influenza, serology of free birds of HAPI areas were investigation and analysis, live stocks and poultries of some areas of Heilongjiang province were investigation and analysis, the HA gene of avian influenza virus A/Goose/XJYL/10/2003HA(H_5N_1)isolated from Xinjiang goose were cloned and sequenced, NS gene of avian influenza viruses (H_5N_1) isolated in Xinjiang were also cloned and sequenced.

本文在调查、分析禽流感发生情况以及影响疫情相关因素的基础上，针对我国野鸟迁徙的2条主要路线，对我国禽流感病毒生态分布和禽流感流行现状的研究，对爆发高致病禽流感疫区的自主活动鸟类禽流感的血清学调查与分析，对黑龙江省部分地区散养畜禽流感病毒感染的血清学调查与分析，并对鹅源H5N1亚型禽流感病毒新疆分离株A／Goose／XJYL／10／2003HA基因的克隆和序列测定与分析，对H5N1亚型禽流感病毒新疆株NS基因的克隆和序列分析。

The thymidine kinase gene of infectious laryngotracheitis virus Beijing E2 strain was amplified by PCR, cloned into pGEM-T vector, and sequenced. Sequence analyses showed that the TK gene was very closed among sequenced ILTV strains.

以纯化的传染性喉气管炎病毒北京E2株基因组DNA为模板，通过PCR方法扩增了ILTV北京E2株的完整TK基因，将其克隆到pGEM-T载体上，并测定了序列，与其它ILTV毒株TK基因比较说明，ILTV不同毒株的TK基因高度保守。

Segments of Adh gene in azuki bean (variety KS 5), covering an interval between partial intron 3 and intron 9, were amplified and sequenced with two pairs of primers. The result has proven the viability of using conserved sequences in a completely sequenced gene of one species to clone same gene in another species. In the comparison between Adh genes of azuki bean and pea, high sequence similarity (about 80%) in exon and long length difference (up to 116 bp) in intron were found. Besides, the edge regions of Adh introns with GT/AG counterparts and more conserved sequences than central regions was observed in both species.

利用两组引子对完成红豆(高雄5号)Adh基因之部分解序，其范围包括部分intron 3至intron 9之间的区域，也印证利用基因之已知保守序列可选殖另一物种之相同基因，红豆与豌豆之Adh基因序列比对结果显示两者之exon均较intron有较高之GC百分比(分别为41.0 ~ 48.7%及23.3 ~ 35.2%)，且两者在exon区域之DNA序列相似程度约80 %，而在intron区域中则有明显的序列差异存在(长度差异最高可达116 bp)，但是intron外侧序列均具有GT/AG组成且相对较中央序列具有保守性。

Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库；(2)经蓝白菌落筛选和斑点杂交，正向消减文库获得307个阳性克隆，反向消减文库获得78个阳性克隆；(3)正向消减文库挑选55个克隆成功测序，经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95％～100％)，这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等；(4)反向消减文库挑选31个克隆成功测序，经同源性检索和比对最终确定差异表达基因片段16个，其中15个与人类全长基因具有很高相似性(95％～100％)，包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性，表明它可能是未被克隆的人类基因EST片段。

Sequencing analysis and database searches indicated that there were 19 known genes and 31 unknown cDNA fragments in the sequenced 72 dot blot positive clones specific for gastrula embryos,and 52 known genes and 37 unknown cDNA fragments in the sequenced 98 dot blot positive clones specific for tail bud embryos.

测序和基因数据库比对结果表明，72个原肠期斑点杂交阳性克隆中，包括19个已知基因的cDNA片段和31个没有同源性的cDNA片段；98个尾芽期斑点杂交阳性克隆中，包括52个已知基因的cDNA片段和37个没有同源性的cDNA片段。

Two types of sockets are possible: stream sockets, which provide bidirectional, reliable, sequenced and unduplicated flow of data; and datagram sockets which do not guarantee that data is reliable, sequenced or unduplicated.

两种类型的插座都是可能的：流套接字，提供双向，可靠，测序和重复数据流量；及数据插座，不保证数据是可靠的，或重复序列。

Database technology, network technology and ASP technology is used in the system. It decompounds standard of equipments sequenced detection in power plant, realizes information management for standard and result of sequenced detection, and offers the support on technology for sequenced detection management and CBM of power plant.

该系统应用数据库技术、网络技术和ASP 技术，对电厂设备检修的点检标准进行了分解，存入点检标准数据库，实现了点检标准和点检结果的信息化管理，系统提供了多种形式的查询功能和面向管理者的维护功能，为电厂设备的点检管理和状态检修提供了技术上的支持。

Graf, without question, is the greatest women's player in history.

格拉芙，现在，毫无疑问是女子网坛历史上最伟大的选手。

If not, you will have to look around at all your important data.

如果没有，您将需要检查所有的重要数据。