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However, the area is less studied than other areas of endophyteresearch world wide. Therefore, a series of experiments were carried out in laboratory,glasshouse and field to compare the responses of endophyte infected plants andendophyte free plants of perennial ryegrass varieties to 10 fungal pathogensisolated from turf type ryegrass in Lanzhou. The pathogens used in the experimentswere Alternaria alternata、Bipolaris sorokiniana、Fusarium avenaceum、Curvularialunata、Ascochyta leptospora、Fusarium acuminatum、Fusarium chlamydosporum、Fusarium oxysporum、Fusarium solani and Gliocladium roseum. In addition, someprotective enzymes activity and alkaloids contents of E+ and E- plants were measuredand LpsA gene of the common strain - Lp19 and mutant strain -AR1 of Neotyphodiumlolii were sequenced to identify their ...

本研究在初步明确兰州市坪用黑麦草内生真菌及其病原真菌多样性的基础上，在实验室、温室和田间开展了系列研究，比较了带内生真菌和不带内生真菌的黑麦草在离体和整株条件下对细交链孢、离蠕孢、燕麦镰孢、新月弯孢、小孢壳二孢、锐顶镰孢、厚垣镰孢、尖镰孢、腐皮镰孢和粉红粘帚霉等10种病原真菌的抗病性，测定了E+和E-植株数种保护酶活性及其生物碱含量的变化。

The internal transcribed spacer region in rRNA gene of Auricularia polytricha was cloned and sequenced, and the sequences were compared with those of some common species of Auricularia.

对毛木耳7个菌株rRNA基因内转录间区进行了克隆测序，并将其与木耳属其它几种的相应序列进行了比较。

The full F gene was sequenced and compared with the published sequences of virulent and avirulent strains of NDV in GenBank database.

根据分离株序列与GenBank中NDV相关毒株序列比较分析，结果表明，所分离的6个分离株归属为3个NDV基因型，其中有基因Ⅶd亚型（3株）、基因Ⅵ型（2株）和基因Ⅲ型（1株）。

The 19 strains of bacilliform bacteria were identified by biochemical tests, part of their 16S ribosomal DNA were amplified, sequenced, and phylogenetically placed.

分离得到得19 株乳杆菌通过菌落、菌株形态观察以及多项生理生化等常规鉴定，并且通过16S rRNA 基因序列测序鉴定，确定这19 株乳杆菌分属于乳杆菌属内专性同型发酵类型中三个不同的种。

Mitochondria DNA extracted from bivoltine silkworm pupae was digested by EcoRⅠ into five restriction fragments,of which the 1.9kb fragment was cloned and sequenced.

克隆了二化性家蚕蛹体的线粒体DNA EcoRⅠ酶解1.9kb片段，并进行了序列测定。

Our study designed two pairs of specific primers and cloned two molecular weight of 63ku from Schizaphis graminum, and Rhopalosiphum maize utilizing PCR technique and have done the prokaryotic expression, the main results are as follows:(1)Amplified the Buchnera groEL of Schizaphis graminum, and Rhopalosiphum maize Yangling Biotype at first time all over the world ,and sequenced the full lenghth gene.

本研究设计了特异性引物，利用PCR技术从玉米蚜和麦二叉蚜中克隆出蚜虫Buchnera groEL基因并进行原核表达研究，主要结果如下：(1)从杨凌生物型玉米蚜和麦二叉蚜中在国内外首先扩增出Buchnera groEL基因，测定了全长基因序列，并对相关的同源基因核苷酸进行了比较。

A banana bunchy top virus DNA components 6 from isolate from Guangzhou were cloned and sequenced.

对香蕉束顶病毒广州分离物MGH DNA组分6全序列进行了克隆及序列分析。

The 5〓 component DNA (DNA5) of the two isolates of banana bunchy top virus were cloned and sequenced.

采用特异性引物PCR扩增方法，分别克隆得到了BBTV NS和NSP株系组分5的全基因序列。

Multiple sequence alignment and phylogenetic analysis of CVX-NTU and other completely sequenced potexviruses demonstrated that CVX-NTU has a close relationship with potexviruses infecting plants in the family Cactaceae.

将CVX-NTU病毒分离株各个ORF与其它已知序列的同属病毒作胺基酸序列比对及类缘分析，结果显示感染仙人掌科的potexvirus都属同一个分群中，显示彼此的类缘关系较密切。

Therefore, NTU isolate is considered as a new CVX isolate and thus designated as CVX-NTU. Multiple sequence alignment and phylogenetic analysis of CVX-NTU and other completely sequenced potexviruses demonstrated that CVX-NTU has a close relationship with potexviruses infecting plants in the family Cactaceae.

当完成NTU全长度核酸序列的解序工作后，将NTU的各个基因转译成胺基酸序列，与已发表的CVX-Hu胺基酸序列作比对，发现两者的RdRP与CP相同度分别达85%和86%，故认定为一新发现之CVX分离株，并取名为CVX-NTU。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。