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The internal transcribed spacer regions of nuclear ribosomal DNA from 8 species of subgenus Amygdalus were sequenced, and analyzed together with other ITS data of 18 species representing subgenus Cerasus, subgenus Armeniaca and subgenus Primus by using of Padus racemosa Gilib. and Padus buergeriana Yüet Ku as outgroup for studying phylogeny of subgenera in stone fruit plants.

采用核核糖体DNA内转录间隔区对桃亚属的光核桃、甘肃桃、新疆桃、山桃、陕甘山桃、扁桃、野扁桃基因序列测序及来源于GenBank的李、杏、梅、樱的18个种的ITS区基因序列，以稠李和楼木、梅和杏分别作为对桃亚属及其近缘亚属植物、桃亚属各种类的外类群，进行系统发育树的建立。

45W related genes were amplified by RT-PCR from the hatched and activated oncospheres of Taenia solium, cloned and sequenced.

利用反转录聚合酶链式反应从激活的猪带绦虫六钩蚴克隆到45W基因家族。

In this framework, image frame, audio and text are represented, which are the three modalities in video shots as data points by the 3rd-order tensor. Then a subspace embedding and dimension reduction method is proposed, which explicitly considers the manifold structure of the tensor space from temporal-sequenced associated co-occurring multimodal media data in video. It is called TensorShot approach. Transductive learning uses a large amount of unlabeled data together with the labeled data to build better classifiers. A transductive support tensor machines algorithm is proposed to train effective classifier. This algorithm preserves the intrinsic structure of the submanifold where tensorshots are sampled, and is also able to map out-of-sample data points directly. Moreover, the utilization of unlabeled data improves classification ability.

在此框架中，视频镜头首先被表示成由视频中所包含的文本、视觉和听觉等多模态数据构成的三阶张量；其次，基于此三阶张量表达及视频的时序关联共生特性设计了一种子空间嵌入降维方法，称为张量镜头；由于直推式学习从已知样本出发能对特定的未知样本进行学习和识别，最后在这个框架中提出了一种基于张量镜头的直推式支持张量机算法，它不仅保持了张量镜头所在的流形空间的本征结构，而且能够将训练集合外数据直接映射到流形子空间，同时充分利用未标记样本改善分类器的学习性能。

PCR products of isolates that rpoB gene mutations were not found with PCR-SSCP were sequenced using double deoxidization chain terminating.

并对其中19株SSCP阴性的耐RFP株用双脱氧末端终止法进行测序分析。

PCR products of isolates that rpoB gene mutations were not found with PCR-SSCP were sequenced usˉing double deoxidization chain terminating.

并对其中19株SSCP阴性的耐RFP株用双脱氧末端终止法进行测序分析。

The PCR products respectively from a donor (TX2), a chronic hepatitis B patient (TX3), a chronic hepatitis C patient (TX4) and a hepatitis patient with unknown etiology (TX1) were sequenced by the dideoxy chain terminating method.

以套式聚合酶链反应测定TT病毒核酸，取献血员(TX2)、慢性乙型肝炎(TX3)、慢性丙型肝炎(TX4)及慢性非甲～戊型，非庚型肝炎患者(TX1)各1例TT病毒PCR阳性产物，以双脱氧核苷酸链终止法测定核苷酸序列。

The 433 base pair segments of the mitochondrial cytochrome oxidase I gene from 62 individuals of Frankliniella occidentalis and other 8 common thrips species in China were sequenced.

用PCR产物直接测序法对入侵害虫西花蓟马和其他8种蓟马的线粒体CO I基因433bp片段测序，获得62个个体的序列。

The 433 base pair segments of the mitochondrial cytochrome oxidase Ⅰ gene from 62 individuals of Frankliniella occidentalis and other 8 common thrips species in China were sequenced.

用PCR产物直接测序法对入侵害虫西花蓟马和其他8种蓟马的线粒体COⅠ基因433bp片段测序，获得62个个体的序列。

Then Dot blot assay was used to select for and amplify differentially expressed cDNAs. Total 22 differential by displayed fragments were cloned and sequenced.

在SSH-NSC富集差异基因的基础上，我们采用了斑点杂交的方法对2个文库进行了差异基因的进一步筛选，共获得22个差异片段，克隆并测序。

Coli genome was amplified by using PCR method. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the Mn-SOD protein expressed by pET-28a vector and purified was injected into rabbits to prepare polyclonal antibody. Western-blot was used to detect the effect of polyclonal antibody.

coli基因组中扩增Mn-SOD基因编码区，将它克隆到原核表达载体上进行大量表达和纯化，再用纯化的蛋白对新西兰大白兔进行背部多点注射，40d后取其血清，用Western-blot印迹实验测定抗体效果。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。