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Methods: The coding region of superoxide dismutase was amplified using PCR method from the E.co1i genome. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the cloned coding region of Mn-SOD was inserted into the expression vector PET-28a to form the recombinant plasmid PET-28a-Mn-SOD and was then transformed into E.coli BL21 for expression.

用PCR方法从大肠杆菌2号基因组中扩增Mn-SOD基因编码区，克隆到pUCm-T Vector，测定核苷酸序列；再将基因编码区克隆到原核表达载体PET-28a，构建含Mn-SOD基因的重组表达质粒，转化到大肠杆菌BL21中进行IPTG诱导表达。

objective: to achieve the soluble expression of mn-sod gene in e.coli and assay the enzyme activity of the expressed product. methods: the coding region of superoxide dismutase was amplified using pcr method from the e.coli genome. the pcr product was cloned into puc19-t vector and sequenced.in addition,the cloned coding region of mn-sod was inserted into the expression vector pet-28a to form the recombinant plasmid pet-28a-mn-sod and was then transformed into e.coli bl21 for expression.

超氧化物歧化酶(superoxide dismutase，sod)是细胞体内歧化超氧阴离子自由基(o-2)的一个抗氧化酶，按其结合的金属性离子根据其中金属辅基的不同可分为4类：cu/zn-sod、mn-sod、fe-sod和ni-sod，它们通过催化超氧阴离子自由基0-2发生歧化反应，达到清除o-2的效果，具有防御氧毒性、增强机体抗辐射损伤能力、防衰老以及治疗某些肿瘤、炎症、自身免疫疾病等功效，广受国内外科研工作者的关注和重视[1]。

Dentium so different from its relatives, Ventura and colleagues sequenced the bug's genome.

dentium与它的同类与此不同，温特勒和他的同僚们排出了这种细菌的基因图谱。

The expression sequence of LSM3 was amplified successfully from muscular tissue of giant panda Ailuropoda melanoleuca using RT-PCR technology for the first time, which was also sequenced and analyzed.

运用RT-PCR技术首次从大熊猫Ailuropoda melanoleuca的肌肉组织总RNA中成功克隆了LSM3的表达序列，并对其进行了测序及初步分析。

In order to clarify the taxonomic status of Dirofilaria sp. of Ailurus fulgens,the second internal transcribed spacer (ITS-2) of rDNA of Dirofilaria immitis from dogs in Ya'an in Sichuan Pro-vince and of Dirofilaria sp.from Ailurus fulgens in Chengdu and Chongqing were sequenced and compared.

为探讨采自小熊猫的恶丝虫虫种的分类地位，测定了四川雅安地区犬源犬恶丝虫和成都及重庆两地小熊猫源恶丝虫的核糖体第二内转录间隔区（ITS-2）基因序列并进行了比对分析。

The avian influenza virus RNA was directly extracted and purified from chicken em-bryo allantoic fluid. According to published gene sequence,a pair of specific primers to the nucleoprotein gene of AIV was designed and synthesized. After that,the NP cDNA was amplified by RT-PCR .Then the complete NP gene was sequenced.

直接从鸡胚尿囊液中提取H9N2亚型禽流感病毒的RNA，并根据已发表的A型流感病毒株的核苷酸序列，设计了1对特异引物，采用RT-PCR技术成功地扩增了AIV的NP基因；将NPcDNA克隆后进行了序列测定。

Mitochondrial cytochrome b gene were sequenced in 44 geese of Chinese domestic geese(15 breeds) and European domestic geese(2 breeds). Anatidae molecular clocks was calibrated with 2 consensus sequences which come from Chinese domestic geese and European domestic geese and 13 sequences obtained from GenBank.

本实验测定了中国家鹅15个品种、欧洲鹅2个品种共44个个体的细胞色素b基因全序列，分别取鸿雁和灰雁家鹅的一致序列与GenBank库中的13条鸟类(鸡形目4条、鸭科鸟类9条)细胞色素b基因部分序列(1045bp)共15条序列构成本研究的数据集。

Non-special primers were designed on the CO Ⅱ genes of Odonata. CO Ⅱ complete genes of 6 genera, including 8 species of 5 families in Anisoptera, had been sequenced and analyzed.

方法］设计蜻蜓目昆虫CO Ⅱ全基因的非特异性引物，获得差翅亚目5科6属8种共8条CO Ⅱ全序列。

In the present study, mitochondrial 12S, 16S and nuclear 18S, 28S rDNA fragments of ten species of spiders were amplified and sequenced. Using neighbor-joining, maximum parsimony, maximum likelihood methods and Bayesian analyses, the phylogenetic relationship between Deinopoidea and Araneoidea was inferred from the combined DNA sequences of 12S, 16S, 18S and 28S rDNA fragments. The resulting phylogeny indicated that: Deinopoidea and Araneoidea did not form single clade.

本文首先测定了包括复杂生殖器类中妖面蛛总科、园蛛总科和非圆网蛛类等3个类群在内的9科10种蜘蛛线粒体12S rDNA、16S rDNA及核18S rDNA、28S rDNA等4个基因片段序列，并基于4个基因序列的整合数据，分别通过邻接法、最大简约法、最大似然法和贝叶斯法分析，对园蛛总科和妖面蛛总科蜘蛛之间的分子系统关系进行了探讨。

Armillaria luteo-virens is a ectomycorrhizal fungus symbiotic with some species of Kobresia in Tibet Plateau, China. A strain was recently isolated from fruitbody tissue of the fungus. To determine whether the strain is the pure culture of the fungus, the internal transcribed spacer region and the first intergenic spacer region (IGS-1) of the nuclear ribosomal DNA of fruiting body and the isolated strain were sequenced and compared. Further, the 5.8S/ITS and IGS-1 sequences of the species were BLAST matched with GenBank database on homology and a phylogenetic tree was constructed based on 5.8S/ITS sequence and two phylogenetic trees were constructed separately based on two IGS-1 sequences.

首次从采自青藏高原、与高原牧草嵩草属Kobresia草本植物形成外生菌根的黄绿蜜环菌Armillaria luteo-virens子实体中分离获得一组织分离菌株，运用rDNA-ITS和rDNA-IGS-1测序技术对该组织分离菌株是否为黄绿蜜环菌的纯培养菌种进行分子鉴定，并基于黄绿蜜环菌的5.8S/ITS和IGS-1序列进行核酸序列数据库GenBank同源性检索比对、构建系统发育树。

Objective:To compare the response control and attention of Schizophrenic patients with that of the healthy controls by Integrated Visual and Auditorycontinuous performance test. To quantitate the impairment of cognitive function in patients, and to explore the relationship between cognitive function and the severity of the disorder.

目的：探讨精神分裂症病人在IVA持续操作测试中的反应控制能力、注意力等，并与健康人进行比较，从而量化精神分裂症病人的认知功能损害，并进一步探讨其认知功能与疾病严重程度的关系。

Main effective factors including subcooling degree, mechanical vibration, gas hydrate reformation, environment temperature, noncondensing gas and surfactant are analyzed.

指出过冷度、机械振动、重复生成水合物、环境温度、不凝性气体、添加剂是影响气体水合物生成的主要因素，还对R152a水合物的放冷进行了实验研究。