查询词典 sequenced

After sequenced the band, we found that no fragment is isogeny with it in NCBI date base. It shows that the DNA fragment was a new cucumber DNA sequence.

将p63-m51-384特异条带进行测序，采用NCBI的Blast软件对GeneBank中的非冗余数据库(GeneBank+EMBL+DDBJ)进行查询，没有发现与该标记片段同源性高于30%的片段，说明该片段为新发现的黄瓜序列。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

This method calculates the next status functions of each trigger in a sequenced circuit and shows all the functions within one Karnaugh map, and then converts into status chart.

该方法首先求出时序电路中各触发器的次态表达式，然后把各次态表达式表示在同一张卡诺图上，最后再把它转换成状态图。

A regulatory element specific for a milk protein is essential for generating mammary bioreactors. This study cloned and sequenced a 1. 59kb DNA fragment derived from bovine β—lactoglobulin gene.

为了制备乳腺生物反应器所需要的乳腺特异性表达的调控序列，本文用PCR法克隆了牛β—乳球蛋白基因的一个1.59kb的片段，它包括0.65kb的5′侧翼序列，第一、二外显子和第一内含子。

The liqase was positively controlled by HAI and AI-2 to some extent. The lecithinase was negatively controlled by HAI-1, and the hemolysin was negatively controlled by both HAI-1 and AI-2. Special primers of luxS gene were designed from the luxS gene sequence of V. harveyi obtained from the GenBank, and the gene was amplified from a pathogenic strain V. harveyi VIB645 by PCR. The luxS gene was cloned to a cloning vector pUC, and was sequenced. It was found that the open reading frame is 519 bp. The similarity of the luxS gene from V. harveyi VIB645 to the published sequence of V. harveyi strain in GenBank was only 90%.

从GenBank中获取哈维氏弧菌中编码AI-2合成酶的luxS基因序列设计一对特异性引物从哈维氏弧菌VIB645中PCR扩增luxS基因，将luxS基因克隆于pUC载体并测序，发现其与GenBank中已发表的哈维氏弧菌的luxS基因序列相似性为90%，证明LuxS/AI-2系统在哈维氏弧菌不同的菌株中的具有一定的特异性，推测LuxS/AI-2系统可能与哈维氏弧菌的致病性或发光现象有关。

silkworm is a model species of Lepidoptera, which genome has been sequenced, has proven to be an excellent experimental system for the study of genetics and mutation.

家蚕作为鳞翅目昆虫的模式生物，是研究遗传和变异的极好的模型。

Methods The differentially expressed genes between liver tumor and non-tumor tissue were cloned by using suppression subtractive hybridization. Differentially expressed genes were sequenced and analized for homology in Gene Bank. In situ hybridization was performed to identify special expression of differentially expressed genes in livre tumor and non-tumor tissues.

方法通过抑制消减杂交法克隆土拨鼠HBV引发的肝癌组织和非癌组织中差异表达的基因；对差异表达基因进行DNA序列测定后于Gene Bank内分析其同源性；应用原位杂交确定差异基因在肝癌组织和非癌组织中的特异性表达。

In this study, the COI gene of mitochondrial DNA from the migratory locust, Locusta migratoria, in China was amplified and sequenced.

对中国飞蝗各亚种线粒体DNA的COI基因片段进行了扩增并测序。

This study examined the SSRs in completely sequenced Bradyrhizobium japonicum, Mesorhizobium loti and Sinorhizobium meliloti genomes in the Microorganisms Tandem Repeats Database.

利用公共的微生物串联重复序列数据库资源，对已测序的3种根瘤菌基因组中SSRs的结构类型、分布、丰度等进行系统的比较分析。

One of the cDNA segments was sequenced. Results showed MTHFR gene was expressed in human B lymphoblast cell line. The cDNA sequence was almost the same as that of human liver cDNA from Genbank.

结果显示永生性人B淋巴母细胞中有MTHFR基因表达，其cDNA序列与文献报道的人肝脏MTHFR基因cDNA序列一致。

"The operators will suffer a temporary decrease in the number of consumers and short message services as people may not like having to provide their ID cards for a phone number," Xu Junqi, vice-dean with the Policy-Making Institute of the Telecommunications Research Centre under MII, said yesterday.

信息产业部下属的通信决策研究院副主任徐俊其昨天说：&实行手机实名制后，人们可能不太情愿用身份证注册手机号，所以通信运营商会暂时面临用户减少和短信用户减少的问题。&

I don't believe in make-believe!

我不相信这些虚构的故事。