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The glycoprotein G gene of pseudorabies virus Hubei strain was amplified by PCR, sequenced and analyzed.

利用PCR技术扩增了伪狂犬病毒湖北株糖蛋白G基因，进行了序列测定和分析。

372Bp mitochondrial cytochrome b DNA of blue fox, red fox, raccoon dog, and wolf were sequenced. Combined with the DNA fragments of dog, simien fox, and African wild dog, sequences were aligned and analyzed. There are 113 nucleotide sites substituted (30%).

通过对犬科的赤狐、蓝狐、貉和狼4种的线粒体细胞色素b约372bp DNA片段序列分析，结合GenBank中狗、西门豺和非洲野犬3种的该区段DNA序列的比较，共发现113个核苷酸位点存在变异(约30%)。

The fact that researchers have sequenced the genomes of all 99 strains of the human rhinovirus is cold comfort for my raw nose.

研究人员已经对所有99种已知鼻病毒的基因组完成了排序。不过这个事实并不能对我发痛的鼻子带来什么帮助。

Eleven differentially exp ressed fragmentswere confirmed by bulked segregant analysis and reverse Northern blotting, further cloned and sequenced.

经过BSA 分析和反向Northern杂交鉴定，获得了11个差异表达的cDNA片段，并进行了克隆、测序和序列同源性检索分析。

A pair of primers based on conservative sequences of Drosophila melanogaster was designed to amplify the p53 gene. The fragment with 1156 base pair was obtained and sequenced. The percent identity between others' and ours' was 99.4%, with the other biological sequence of p53 existing some differences.

从紫外刺激的果绳抽提总RNA，通过RT-PCR方法扩增得到有完整读码框的p53基因，并且通过对p53基因的序列分析表明，所得到的序列与网上已经登陆的果蝇p53序列有99.4%的相似系数，而与其他生物的p53序列存在一定的分歧。

In this paper, the fib-H promoter was choned by PCR method and sequenced. Sequence analysis showed that the cloned fib-H promoter harbored the typical motifs, which resulted in the gene expressing in the silk gland specifically.

为利用其时空调控机制，通过PCR扩增方法克隆fib-H启动子片段，并进行序列分析，进而构建由fib-H启动子控制报告基因DsRed的重组载体pSK-FH-DsRed-PolyA，通过家蚕BmN细胞和丝腺进行瞬时表达。

In this study, the cDNA fragments of G-L intergenic region and polymerase activity module in L gene of two street rabies virus were cloned and sequenced. The nucleotides sequence of these cDNA fragments mentioned above and their deduced amino acid sequence were compared with that of rabies virus strains, rabies-related virus strains and other Rliabdoviridae published previously. The result show that the homology of nucleotide sequence of G-L IGR among MRV, DRV is 83.3%, compared with other rabies-related virus strains, the homology of G-L IGR is between 58.3%-91.7%. the length of G-L IGR of rabies virus is different in Rhabdoviridae.

本实验对狂犬病病毒野毒株MRV、DRV的G-L间隔区以及L基因聚合酶活性部位进行了克隆和测序，将所测定的结果和推定的氨基酸序列与国内外公开发表的狂犬病病毒其它固定毒株、野毒株和同科不同属的病毒的相应部分进行了分析比较，结果表明MRV、DRV G-L IGR核苷酸序列的同源性为83.3％，与其它毒株的同源性在58.3％～91.7％之间；狂犬病毒G-L间隔区序列的长度在弹状病毒科不同病毒属之间有所不同。

In this study,two recombinant plasmids harboring non-structural and structural protein gene named pMD18-T-NS1 and pMD18-T-NS1 were constructed and sequenced.

但这种诊断试剂和亚单位疫苗的研制，必须建立在对病毒蛋白抗原性的研究之上。

Objective In order to make a foundation for molecular diagnosis and gene engineering study of Avian Encephalomyelitis Virus, we cloned and sequenced the structural protein VP3 gene of AEV.

目的 对VP3进行克隆和测序，为禽脑脊髓炎病毒的分子诊断以及更深一层次的分子和基因工程研究打下基础。

Objective In order to make a foundation for molecular diagnosis and gene engineering studies of Avian encephalomyelitis virus, the structural protein VP3 gene of AEV and expression of its recombinant protein were cloned and sequenced.

目的对VP3进行克隆和测序后，在杆状病毒系统中表达获得重组蛋白，为AEV的分子诊断以及更深一层次的分子和基因工程研究打下基础。

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。