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By using PCR method, a 1kb fragment was gained from Heliothis amigera nuclear polyhedrosis virus genome. The whole fragment was sequenced by dideoxided chain termination methods and a full open reading frame which encode 299 amino-acids was found. The molecular weight of the peptide is about 35 kilodalton.

用PCR方法从棉铃虫核多角体病毒基因组中扩增到了1kb的DNA片段，用双脱氧链终止银染测序法测定了该片段的全序列，发现它含有一个完整的开放阅读框，编码299个氨基酸残基的多肽，分子量约35KD。

TTV DNA isolated from sera of 6 hemophilic patients by polymerase chain reaction were cloned using TA easy vector. 3-5 clones selected from each patient randomly were sequenced, and then compared with TTV DNA sequences in GenBank.

对6例血友病人血清中分离的TTV进行基因克隆，从每例病人的标本随机选多个克隆进行测序，比较其核苷酸和氨基酸序列。

Methods The 26th exon, the 30th exon and the 21st intron of gene GP Ⅱb in 110 individuals were amplified by polymorphism and sequenced to investigate whether there was linkage among the polymorphisms of the gene. Human platelet antigen-3 (HPA-3) gene frequency was detected by Fok 1 enzyme in 147 patients with hematologic diseases, and was compared with that in 110 normal individuals. Forty-four patients who received apheresis platelet transfusion repeatedly were randomly divided into the HPA-3 homotype group and the control group. The antibodies of the platelet were detected after 3 times of platelet transfusion.

聚合酶链反应－单链构型多态性分析检测110例正常人GP Ⅱb基因第26和30外显子(Exon 26， Exon 30)及21内含子(Intron21)基因多态性，进行基因序分析，研究这些基因多态性是否存在连锁关系；应用Fok1酶切法对147例血液病人人类血小板抗原－3(human platelet antigen-3， HPA-3)基因进行分型，并与110例正常人进行比较；将接受单采血小板输注的44例血液病人随机分为HPA-3同型输注组和对照组，血小板输注3次以后检则血小板抗体。

Eight differential expression bands were cloned and sequenced.Blastn analysis showed that seven of them had highly homology(81%～100%)with EST sequences of Triticum aestivum and Hordeum vulgare.Six of the seven sequences came from Triticum aestivum,and one sequence came from Hordeum vulgare.

对上述5种特异表达类型中8条特异表达片段进行序列测定和分析，在相应的数据库，经Blastn比对，有7个序列在数据库中找到同源序列，且与普通小麦和大麦中的EST序列有较高的同源性，同源性为81%～100%，其中有6个来自普通小麦，1个来自大麦。

Based on the degenerated primer, the cDNA of open reading frame of growth horme gene for black carp and Japanese crucian carp were cloned and sequenced through RT-PCR, which were used for the"all-fish"transgene construction and preparation of polyclonal antibody against GH for black carp.

基于鲤科鱼类生长激素基因序列，通过RT-PCR技术，克隆了青鱼、日本白鲫生长激素基因编码区cDNA序列，以此来构建&全鱼&基因载体和研制生长激素多克隆抗体。

The multi-drug resistance rate of Salmonella and E.coli were 91.80% and 100%.According to the genes sequence published in Genbank ,we analysed the value of Tm ,content of G+C, oligonucleotide dimer by DNAStar and Primer 5.0 software, and according to the construction of I integron ,we designed 3 pairs of primers to amplify intIl gene ,variable segment ,3"-conserved segment for detectting Salmonella and E.coli"s I integron.As a result , aimed genes were amplified and sequenced;The detection rate of I integron of 61 Salmonella and 48 E.coli were 81.97% and 66.67%.

据文献报道及GenBank中注册的基因序列，运用DNAStar软件分析同源率及Primer5.0软件对Tm值、G+C含量、引物二聚体等进行分析，根据Ⅰ类整合子的结构设计了3对引物，对Ⅰ类整合子的intI1、可变区、3'保守端进行扩增，检测沙门氏菌和大肠杆菌Ⅰ类整合子；扩增出了相应基因片段并测序；61株沙门氏菌和48株大肠杆菌中分别有50株和32株菌检测出Ⅰ类整合子，Ⅰ类整合子的检测率分别为：81.97％和66.67％。

ITS+5.8S rDNA region of Ulva lactuca, Ulva fasciata, Monostroma nitidum and Enteromorpha prolifera were sequenced and compared with seven samples from GenBank. The phylogenesis of different green algae was also analyzed.

通过分析石莼，裂片石莼，礁膜和浒苔样品的ITS+5.8S rDNA区序列，与GenBank中相应序列进行比对，分析了多种绿藻的系统发育关系。

The complete sequences of mitochondrial Cyt b gene in Pagrus pagrus , Sparus macrocephalus , Acanthopagrus latus and Parargyrops edi ta in Zhoushan sea area were amplified, cloned and sequenced.

克隆测序了分布于舟山海域的真鲷、黑鲷、黄鳍棘鲷和二长棘鲷4种鲷科鱼类的线粒体Cyt b 基因1 141 bp的全序列。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis ×L. principis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds randomly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松×华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis L. princi-pis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds ran-domly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

When this condition occurs, inbound replication with the source partner is stopped on the destination domain controller and event ID 2042 is logged in the Directory Services event log.

计算机密码学是研究计算机信息加密、解密及其变换的科学，是数学和计算机的交义学科，也是一门新兴的学科。

Instructions: click on the thumbnails to see a larger image, then use the left-right arrow keys to scroll through the slideshow.

使用说明：滑鼠点在小图上即可放大观赏。开启后键盘左右键可用来换照片。