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We selected mecA gene and femA factor asβ-lactam antibiotic-resistant genes, ermA, ermC, msrAgene as macrolides-lincosamids antibiotic-resistant genes, norA, grlA, gyrA, gene as Quinolonesantibiotic-resistant genes, and tetM gene as Tetracycline antibiotic-resistant genes on the basis of the latestresearch. We designed primers with GenBank enunciable gene sequence, cloned the drug resistantgenes, and sequenced these genes.

根据国内外最新文献报道，筛选出对β-内酰胺类抗生素耐药的mecA基因及辅助因子femA；对大环内酯类及林可霉素类抗生素耐药的ermA、ermC、msrA基因，对喹诺酮类抗菌药物耐药的norA、grlA和gyrA基因及对四环素类抗生素耐药的tetM基因作为拟选的主要耐药基因，分别设计PCR扩增的特异性引物，进行克隆。

A mesophilic eubacterium, among all fully sequenced prokaryotic genomes up to date. Computational analysis on genes involved in basic metabolic pathways supports the experimental discovery that T.

结果显示，基因组大小为2，689，445-bp（Genbank Accession NumberAE008691），包含2，588个编码序列（coding sequences，CDS），其中1，764个CDS（68.2％）与已知蛋白质的序列同源，其余824个CDS（31.8％）的功能未知。

Thermoanaerobacter tengcongensis is a rod-shaped, Gram-negative, anaerobic eubacterium that was isolated from a fresh water hot spring in Tengchong, China. Using a"whole-genome-shotgun"method, we sequenced its 2, 689, 445-bp genome from an isolate, MB4〓(Genbank Accession Number AE008691). The genome encodes 2, 588 predicted coding sequences . Among them, 1, 764 (68. 2％) are classified according to homology to other documented proteins, and the rest, 824 CDS (31. 8％), are functionally unknown. One of the interesting features of the T. tengcongensis genome is that 86. 7％ of its genes are encoded on the leading strand of DNA replication.

中文题名腾冲嗜热厌氧菌全基因组测序及分析副题名外文题名 A complete sequence of the T.tengcongensis genome 论文作者包其郁导师杨焕明研究员学科专业遗传学研究领域\研究方向学位级别博士学位授予单位中国科学院遗传与发育生物学研究所学位授予日期2002 论文页码总数88页关键词腾冲嗜热厌氧菌基因组嗜热菌馆藏号BSLW /2003 /Q939.1 /33 腾冲嗜热厌氧菌（MB4＇）是分离于我国腾冲地区温泉内的革兰氏阴性厌氧真细菌。

The combined analysis of the four cpDNA sequences showed similar topology to that in matK trees.2 Mitochondrial matR gene sequence analysisThe mitochondrial gene matR of 28 species representing 28 genera of Fagales, and 2 outgroups was sequenced.

3基于核核糖体185 rDNA序列的分析在185 rRNA基因最简约树的严格一致树上，壳斗目被分为两支，一支由壳斗科和南青冈科组成，另一支由核心高等金缕梅类组成，BS支持率均不高。

We have sequenced the 90 megabase genome of the human filarial parasite Brugia malayi and predict 11,500 protein coding genes in 71 Mb of robustly assembled sequence.

报告中指出，一般人都是被带有寄生虫的昆虫或蜘蛛咬到后感染，这种寄生虫会在人体内造成淋巴液增生，导致头、四肢和神经血管肿大。

Several dozen researchers, led by Rebecca Garten of America's Centres for Disease Control and Prevention, sequenced full or partial genomes of 76 samples of the new virus, which has afflicted almost 13,000 people around the world so far.

在四月份出现了一种具有大规模流行病爆发可能性的流感病毒，我们一般把它称之为&猪流感&，这是因为这种病毒和存在于猪体内的A/H1N1病毒类似。

Several dozen researchers, led by Rebecca Garten of America's Centres for Disease Control and Prevention, sequenced full or partial genomes of 76 samples of the new virus, which has afflicted almost 13,000 people around the world so far.

在丽贝卡。加滕的带领下，美国疾病控制中心几十名研究人员对新病毒的76个样本作了全部或部分基因排序。迄今在全球范围内，甲型H1N1病毒已经导致13，000人感染。

Several dozen researchers, led by Rebecca Garten of America's Centres for Disease Control and Prevention, sequenced full or partial genomes of 76 samples of the new virus, which has afflicted almost 13,000 people around the world so far.

美国疾病控制和预防中心（America's Centres for Disease Control and Prevention）的Rebecca Garten领导的几十名研究者测定了这种新病毒的76个样本全部或者部分的基因组序列。这种新的病毒到目前为止致使全球将近13000人染病。

Several dozen researchers, led by Rebecca Garten of America's Centres for Disease Control and Prevention, sequenced full or partial genomes of 76 samples of the new virus, which has afflicted almost 13,000 people around the world so far.

目前全世界已经有13，000位病患深受新型流感之苦。美国的疾病控制与预防中心 America's Centers for Disease Control and。

MtDNA COⅠand COⅡgene fragments of Haliotis ovina and H. asinina were amplified by PCR, and the PCR products were purified and sequenced.

采用基因测序的方法，对我国海南产的羊鲍和耳鲍两个自然种群的COⅠ和COⅡ基因片段进行了PCR扩增和测序。

Who? I never heard of him, Paul said, before asking teammate James Posey if he had heard of him.

赛后，科比说，他一直都是一名非常出色的射手，今天他打得很棒。

When I joined the company, I rotated around the different sections.

我加入这个公司时，轮换过几个不同的部门。