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This research is taking sea perch as the material, and cloning the Syntaxin 1B gene. After extracting RNA from sea perch tissue, we gained the first chain of cDNA by RT-PCR. Then designing a pair of primers according to the sequence of Syntaxin 1B from other organism. Based on the first chain of cDNA cloned from sea perch, the partial sequence of Syntaxin 1B gene is been cloned by using TD-PCR and we received the 751bp fragment at last.

本实验从鲈鱼脑组织中提取总RNA，通过反转录得到cDNA第一条链，根据已克隆出的其它生物的Syntaxin 1B基因序列，设计并合成引物，在妒鱼syntaxin1B基因部分序列克隆与分析 cDNA第一条链的基础上使用降落PCR，进行体外扩增妒鱼 Syntaxin IB基因的部分序列，得到长度为75 lbP的片断，在胶中洗脱下该片段，与pMD 18一T载体链接，转化入感受态细胞，挑取阳性克隆扩大培养，提取质粒，经HindIH和x一gaH两内切酶消化得到75 1 bp的片断，证明链接成功。

Rita smoothed suntan lotion onto her shoulders as she watched her niece sitting cross-legged in the sand, 1poking a shell fragment with someone ' s discarded straw.

丽塔一边往肩上抹着防晒油，一边看着她的外甥女。小姑娘盘腿坐在沙滩上，正用别人扔掉的吸管拨弄着一个贝壳残片。

This volume: A bomb explodes in a Salt Lake City office block, police respond immediately, a man got killed, but this detonation was not last time, dispersed continually along with the bomb fragment, Salt Lake City fell into the fear.

本集：一颗炸弹在一栋盐湖城办公大楼爆炸，警察随即作出回应，一名男子丧生，但这次爆炸不是最后一次，随着炸弹碎片持续飞散，盐湖城陷入恐惧之中。

Some measures of lessening large fragment and toe rock in medium-length hole blasting as well as their effects in Yongping Copper Mine are described.

综述了制备非晶的各种方法，介绍了大块非晶的制备途径，对一些方法特点进行了讨论，并对它们进行了初步对比。

Samples of 80 and 17 ring-necked pheasants were collected from the wild and domestic farms, respectively for an investigation of their genetic make-up. A fragment of 464bp of the control region in the mitochondrial genome was chosen for the analysis.

采集自野外和人工养殖的环颈雉共97只个体，分析长度464bp的粒线体控制区DNA序列，发现有24个变异位置，皆为碱基替代，可以定义出13个不同的基因型。

To elucidate the mechanisms regulating TSHb expression, we isolated a 5.1-kb fragment of the red-spotted grouper 5'-flanking region by Genome walking .1.8kb of this upstream region was used to produce GFP reporter construct (pBK-TOL2) for analysis of tissue-specific expression in zebrafish embryos.

将起始密码子上游1864bp的启动子片段顺向克隆到pBK-TOL2载体中， pTSHb-TOL2重组质粒与转座酶mRNA共注射斑马鱼一细胞期受精卵，荧光显微镜观察发现，TSHb启动子驱动GFP在原始生殖细胞和垂体中表达。

Fig.3 Restriction analysis of fragments of PCR amplification of RH,ZS2,CN isolates1.PCR Markers 2.Amplification fragment of GRA1 gene of RH isol?

2.3 弓形虫RH株、ZS2株、CN株扩增产物的HpaⅡ酶切鉴定 GRA1基因的酶谱分析表明，HpaⅡ位点在274bp和444bp处，该酶切GRA1后应该有444bp、274bp和170bp 3个片段。

Intercutting may also be used to create a montage sequence--a series of brief shots that show different details of an action that occur simultaneously or that show fragment s of variety of events related to one another by the story.

交切也可用来剪辑一系列蒙太奇镜头--一系列表现同一动作不同侧面的画面或者表现一些相关事件不同侧面的画面。

It was found that one of the fragments was similar to the Peroxidase gene located on the rice chromosome 1 completely, another fragment was partially similar to the protein Phosphorylase gene located on the rice chromosome 12 with 99.99% homology.

同源性均在99％以上。其中一个与位于水稻第一号染色体的过氧化物酶基因的部分片段有100％相似性。另一个与位于第十二号染色体上的蛋白磷酸化酶基因有99.99％的同源性。

HPLC/MS/ MS was used to analyse the nucleosides in Cordyceps sinensis .and its counterfeits and adulterations. Compared the samples with the standard samples,the result showed that the main fragment chromatogram were similar.The four nucleosides in Cordyceps sinensis .and its counterfeits and adulterations were identified by MS/ MS.The totle content of uridine、hypoxanthosine、guanosine、adenosine in Cordyceps sinensis .was from 2.473 mg/g to2.830 mg/g ,the content of adenosine was from 0.850 mg/g to1.009 mg/g .

应用HPLC-MS对冬虫夏草及其混淆品与伪品中的四个核苷成分进行二级质谱定性分析，并与对照品进行比较，结果显示，冬虫夏草及其混淆品与伪品均能产生与相应对照品一致的主要碎片离子峰，进一步说明，冬虫夏草及其混淆品与伪品中均含有尿苷、肌苷、鸟苷及腺苷四种核苷成分。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。