查询词典 fragment

We found that neither the fusioned nor the non-fusioned forms of Hu-P68 cDNA open reading frame could express efficiently in the E. coli cells; in different prokaryotic expression vectors and under various promotors resulted in no distinctive variations. Deletion of 5'UTR by synthesis adaptors, deletion of 3'UTR or signal peptide sequence by PCR gained no gross improvement. Only the truncated API-I proteins were observed to be highly expressed when the 3'ORF fragment of the Hu-P68 cDNA was fusioned at the 3'end of PAI-I gene.

结果发现无论是以融合形式存在，还是以非融合形式存在，人的P68 cDNA基因在E.coli细胞中都未能获得高效表达：在不同表达载体中，不同启动子对P68 cDNA基因的表达没有显著差异；利用合成Adapter的方法删除5'UTR，及利用PCR技术删除3'UTR和信号肽序列对表达没有明显改善；分别表达被分割的P68 cDNA5'端编码区、中央编码区和3'端编码区均未见有明显表达；当P68 3'端编码区与高效表达蛋白PAI-Ⅰ基因3'末端融合时，仅见有PAI-Ⅰ以截断形式高效表达出来。

Only the LASS2 protein carboxyl terminal hydrophilic fragment could be expressed in the prokaryote expression system and its polyclonal antibody was produced.

在原核表达系统中仅能够表达其羧基端胞外区片段却不能表达完整的LASS2蛋白，并制备了该片段的抗体。

After Huang Qu senior official yellow Jinlong looking pensive stopping, also in a low voice saying:"Knows, on the Earth 60,000,000 year ago dinosaur exterminate, it is said is because at that time a diameter reached 200 kilometers planetoids to hit Saturn high speed, caused the Saturn entire stratum and the earth's crust receives the undulation, and caused the fierce reverse volcanic eruption, finally acted according to the near several centuries the continually exploration research to indicate, Saturn periphery several satellites, and even the giant beautiful saturn's ring, possibly all was creates by this direct hit, moreover most surprising was, spouted the Saturn parent substance together after the burning hot Saturn lava material coagulation, formed the diameter reached 30 kilometer hit fragment, after is hit unexpectedly Saturn fierceSways the movement, flung the Saturn orbit rapidly ......"

黄区长官黄金龙若有所思的停了停之后，又低声的说道：&知道吗，地球上6000万年前的恐龙灭绝，据说就是由于当时一颗直径达200千米的小行星高速撞击土星，导致土星整个地层和地壳受到波动，并引起剧烈的反向火山喷发，结果根据近几个世纪的持续探索研究表明，土星外围的几个卫星，乃至巨大美丽的土星光环，可能都是由这次直接撞击造成的，而且最令人惊讶的是，一块喷出土星母体由炙热的土星熔岩物质凝固后，形成的直径达30公里的撞击碎块，竟被撞击后土星剧烈的摇晃运行，急速甩出了土星轨道……&

Over 1500 measurements were made to evaluate K-wire fragment and screw position in the 116 instrumented pedicles for which CT data were available.

通过对116个植入器械的椎弓根的CT扫描得到了超过1500个数据，用来评价短克氏针和螺钉的位置。

The techniques for IR percutaneous K-wire fragment and percutaneous screw placement evolved over the duration of the study. Instrumentation was placed in 126 pedicles in 25 patients.

随着研究的进行，透视下经皮短克氏针和经皮螺钉的植入的技巧随之提高。25例病人，126个椎弓根内植入了器械。

A fragment of the wonderful system which they built up survives in the Pandects of Justinian, and supplies evidence that their works took the form of treatises on all parts of Roman Law, but chiefly that of commentaries on the Edict.

他们所建立起来的奇伟制度，在查斯丁尼安&法学汇纂&中还保存着一些片段，证明他们的著作采用了论文的形式讨论了&罗马法&的全部，但主要的是对&告令&加以解释。

6 Kb of pGFAP-Cre-hGH DNA fragment was introduced into 191 fertilized eggs by microinjection. 176 injected eggs were implanted into the oviducts of 8 female mice respectively, from which 25 offspring were obtained.

以显微注射的方法将7.6 kb的转基因片段pGFAP-Cre-hGH引入191枚小鼠基因组受精卵，其中176枚分别移植至8只假孕母鼠的输卵管中使其发育，共获得子代小鼠25只。

6 Kb of pGFAP-Cre-hGH DNA fragment was introduced into 191 fertilized eggs by microinjection. 176 injected eggs were implanted into the oviducts of eight female mice respectively, from which 25 offspring were obtained.

以显微注射的方法将7.6 kb的转基因片段pGFAP-Cre-hGH引入191枚小鼠基因组受精卵，其中176枚分别移植至8只假孕母鼠的输卵管中使其发育，共获得子代小鼠25只。

Ostinato is an Italian music term that means a recurring melodic fragment.

&Ostinato&这个词是一个意大利音乐术语，意思为&循环的旋律片段&。

Methods Human EGFR cDNA fragment was subcloned in the oppos ite orientation to the cytomegaloviral promoter and inserted into a E1/E3-delet e d type 5 adenoviral vector to obtain AdE5 construct which expresses EGFR antisen se RNA.

人EGFR的cDNA片段被反向亚克隆入E1/E3缺失的 5型腺病毒载体中而得到AdE5 ，EGFR反义RNA的表达由CMV启动子控制。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。