查询词典 modifier gene

For the purpose of constructing mammary gland specific expressional vector, we use the updream regulation sequence of bovine BLG to regulate lacS gene to express in mammary gland. Positive clone cells are selected and purified by the expression Neo gene and report gene EGFP. So the cells can be used as sources to create transgenic animals by nuclear transfer techniques.

本研究以牛β-乳球蛋白基因上游调控成分作为启动子，嗜热菌糖苷酶基因lacS为目的基因，构建了lacS基因乳腺特异表达载体，并通过脂质体介导法转染体外培养的牛胎儿成纤维细胞，借助新霉素抗性基因和增强型绿色荧光蛋白报告基因来筛选、纯化阳性克隆细胞，为利用核移植技术制备转基因动物提供细胞来源。

Using the total RNA of merozoites of Eimeria acervulina isolated from Guangdong Pro- vince as template,a partial segment of 3-1E gene was amplified by RT-PCR. The gene fragment 682 bp inlength was cloned into pGEM-T Easy vector,and the recombinant plasmid was identified by PCR,restric-tion enzyme analysis and sequencing. The homology analysis revealed that the nucleotide homology be-tween the 3-1E gene of the E.

根据GenBank中登录的堆型艾美球虫3-1E基因序列，设计了3条引物，以广东株堆型艾美球虫裂殖子总RNA为模板，利用反转录-聚合酶链反应扩增获得了3-1E基因部分片段，将这一片段克隆至pGEM-T Easy载体中，经PCR、限制性内切酶分析和克隆片段的序列测定、比较，证实了克隆片段的可靠性。

Many childhood leukemias result from mutations called translocations, where gene pieces on chromosomes accidentally relocate and misalign. In infant leukemia, the chromosome containing the MLL gene breaks within MLL and ends up fused to a different gene.

很多儿童白血病是由一种被称为易位的基因突变（染色体上基因片断突然移位重排）引起的，在幼儿白血病中，含有MLL的染色体破损，然后融合到其它基因上。

At the same time, the process of the wound healing was observed histologically, and the regeneration cycle of epidermal cells and the temporal change in inflammatory cells were measured. Results Inflammatory cells infiltrated into wound surface were neutrophils, followed by macrophages and lastly lymphocytes. Epidermal cells proliferated most actively on PBD 3 and the mitoses of them increased significantly on day 7 after burn. The gene expression of PDGF, PDGFR and EGFR reached peaks on PBD 1 and the gene expression of EGF and TGFβ-R2 were highest on PBD 3. In addition, the gene expression of TGFβ-R1 and TGFβ1 increased significantly on PBDs 5 and 7 respectively.

结果 炎性细胞到达创面依次为中性粒细胞、巨噬细胞和淋巴细胞；表皮细胞周期变化为伤后3天细胞增殖活跃，伤后7天细胞分裂明显增多；伤后1，3天PDGF、PDGFR(血小板源性生长因子受体)和EGFR基因表达明显增强，伤后3，5天EGF、TGFβ-R2基因表达增强，伤后5，7天TGFβ-R1(转化生长因子β受体1)基因表达增强，伤后7，10天TGFβ1基因表达增强。

This developed a new material for the wheat virus-resistance breeding.In order to breed maize dwarf mosaic disease梤esistance maize species, the PAP gene was introduced into maize inbred strains and hybrid strains mediated by pollen and particle bombardment,10 transgenic maize plants were achieved and the PAP gene was indeed integrated into the maize genome by analysis of molecular biotechnological methods. The PAP gene in one of the transgenic maize plants had been transcription and translated, the ability of the virus resistance is undergoing.

为了解决玉米矮花叶病害的防治问题，采用花粉介导法及基因枪法将PAP基因分别导入玉米的杂交种及自交系，获得了10株转基因玉米，PCR及Southern-blot检测结果表明PAP基因已经整合到了玉米基因组中，并且其中有一株PAP基因已经得到了表达，病毒抗性鉴定表明转基因玉米能明显推迟玉米矮花叶病害症状的表现。

The results indicated that the c-terminal fragment of Arabidopsis CRY1 gene was successfully integrated into the rape genome, mainly in the form of tabling, with a few in the form of single copy. The transformed gene could be stablytransmitted in the progeny, with a transmitted line rate of 62.5%. The genetic pattern of the transformed gene in the T_2 generation was mostly non-Mendelian. The transgene was expressed in some of the transgenic plants, but kept silent, in other plants.

结果表明，转化的外源目的基因多数以嵌合形式，少数以单拷贝形式整合到转基因甘蓝型油菜的基因组中；目的基因能稳定地遗传给后代，株系间遗传传递率为62.5％；T_2代的遗传行为少数呈现孟德尔遗传，多数呈现非孟德尔遗传现象；拟南芥CRY1基因能在转基因油菜中表达，但也出现了沉默现象。

Results: MMP2 gene expression could be detected in norma l subject. MMP2 gene expression levels in patients with chronic hepa titis and liver cirrhosis were higher than that of normal control. Th e gene expression level in patients with chronic hepatitis was higher in comparison with that in patients with liver cirrhosis.

结果：正常人有明胶酶A(MMP2)的基因表达；慢性肝炎、肝硬化患者MMP2基因表达较正常对照明显增强；慢性肝炎患者MMP2基因表达水平明显高于肝硬化患者。

The stage which is most sensitive to high temperature is trachea colouring embryo(ji-3), while the stage sensitive to dry condition is body pigmentation embryo(ji-5), so these two development stages have been called high temperature sensitivity stage and dry condition sensitivity stage respectively. The gene of high temperature and dry condition sensitivity is defined as sex-linked temperature sensitivity gene, which exists in 21.5 location of Z chromosome linking closely to sch gene, and presents concealed heredity.

其中对高温最敏感的时期是气管显现期(己_3)，对干燥最敏感的时期是转青期(己_5)，这两个时期分别称为高温敏感期和干燥敏感期，高温干燥敏感性基因定义为伴性温敏基因，t与Z染色体上21.5位置的sch基因紧密连锁，呈隐性伴性遗传。

A/DK/Hubei/216/83(H7N8) shared 96%～98%homology with different subtypes avian influenza viruses which isolated from HongKong during 1972～1997, in addition, evolutionary analysis showed that the remainingfive gene except NA and PB2, had close phylogenetic relationship with 1972～1997strains. A/DK/Hubei/137/83(H10N4), sequence similarity analysis revealed that theNS, PA, PB1, PB2 gene shared 98%～99% homology with the 1997～2004 H5N1subtype avian influenza virus strains, and except NA and M gene, the other five geneformed independence branch with their own, and the distance between the otherisolates were far.

前者HA氨基酸剪切位点序列为P-E-V-V-Q-G-R，后者HA剪切位点序列为P-E-I-P-K-R；2株病毒的NA在第48位氨基酸后无20个氨基酸的缺失；NS蛋白的第79～84位也没有氨基酸的缺失，不具有高致病性特征；A／DK／Hubei／216／83(H7N8)与香港1972～1997年不同亚型AIV的同源性为96％～98％，进化分析表明，除NA和PB2独自成一支外，其余5个基因都与1972～1997年毒株亲缘关系较近。A／DK／Hubei／137／83(H10N4)除NA和M基因外，其他5个基因分别独立自成一支。

To investigate the methods to effectively and simply assess the CAG repeat size of HD gene which was necessary for gene diagnosis of Huntington disease, the sequence including polymorphic CAG repeat of HD gene was amplified by PCR with TaKaRa LA Taq DNA polymerase and GC buffer. PCR products were analyzed on polyacrylamide gel to distinguish normal alleles from HD alleles. The DNA fragments of affected alleles were recovered from polyacrylamide gel as templets for secondary PCR. The secondary PCR products were cloned into T vector for sequencing analysis to determine CAG repeat size. A total of 20 normal individuals and 3 members from a HD pedigree were included in this study.

为了简单高效检测HD基因开放阅读框5'端n三核苷酸重复序列，建立快速准确的亨廷顿病(Huntington disease， HD)基因诊断方法，应用TaKaRa LA Taq DNA聚合酶配合GC buffer扩增HD基因包含n重复序列的目的片段，非变性聚丙烯酰胺凝胶电泳检测后回收n拷贝数异常增多的目的片段，再次PCR扩增后将产物连接至T载体，进行DNA测序确定CAG的拷贝数。

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Tara appears in Buddhism, Jainism, and particularly, Tibetan Lamaism, as a complex array of manifestations: goddess of ascetism and mysticism, mother creator, protectress of all humans as they cross the sea of life.

度母出现在佛教，耆那教里面，尤其是西藏的喇嘛教，作为一系列的陈列阵容；神秘的女神，创世之母，人类横过生命之海洋的女性保护者。