查询词典 modifier gene

Selecting the better amplified from Diacylglycerol acyltransferase, gene, Acyl- desaturase gene ,then cloning sequencing, afer that, through the NCBI and nucleotide sequence to match homology , the results show that: cloning by the cassava, castor-oil plant Jatropha curcas DGAT and SAD genes and gene fragments have the high homology with other known plant DGAT genes and SAD genes .

从中筛选出扩增效果较好的二基甘油基转移(Diacylglycerol acyltransferase， DGAT)基因，硬脂-基载体蛋白脱饱和(Acyl- desaturase， SAD)基因克隆测序，经测序，通过NCBI与已知的核酸序列进行同源性比对，结果表明：克隆所获得的木薯、蓖麻和麻疯树 DGAT基因和SAD基因的片段与其它已知植物的DGAT基因和SAD基因具有很高的同源性。

Label with LC-Red640 and LC-Red750 two are aimed at above all 112 code with 158 child detect 5 ′ carry bougie, corresponding anchor decides bougie to be labelled by fluorescence in 3 ′ end, circulate quickly from inside DNA of person gene group again PCR is distinctive the enlarge that make thing adds part of ApoE gene 265bP and with distinctive bougie cross hind, cause fluorescent resonance energy to transfer, the occurrence that passes different source cross and fault match alkaline base the fusible parameter of the type will finish feral model divide with choppy gene model.

首先以LC-Red640和LC-Red750标记两条针对112和158位编码子的检测探针5′端，相应的锚定探针在3′端被荧光标记，再从人基因组DNA中快速循环PCR特异引物扩增apoE基因265bP片段并和特异探针杂交后，引发荧光共振能量转移，通过异源杂交的出现和错配碱基的类型的熔解参数来完成野生型和突变基因分型。

In the functional mechanism, we should use the technique "use the DNA Microarray to build the gene expression difference chart" to lead to the combination of Traditional Chinese Pharmacy and gene expression difference chart to study. Meanwhile,we can establish a totally new method of screening modern Traditional Chinese Pharmacy based on the gene expression difference chart, it can really make the modernization and internationalization of Traditional Chinese Pharmacy.

在中药作用机制方面，应采用&利用基因芯片建立基因表达差异谱&技术，将中药与基因表达差异谱有机地结合起来进行研究，同时以基因表达差异谱为基础建立一种全新的现代中药筛选方法，使中药真正实现现代化、国际化。

An expression construct specified in mammary gland with double cistrons of human G-CSF gene and IRES-EGFP gene under control of ovine beta-lactoglobulin gene flanking sequence,has been constructed in two parts (named fragment I and Fragemnt II)that share an overlapping region of 2.2 kb sequence.Two sites of loxp and lox2272 for homologous recombination were inserted into both flanking regions of G-CSF.The lengths of fragment I and Fragment II are 5.9kb and 5.6 kb,respectively.The whole length of the expression vector (β-LG-hG-CSF-IRES-EGFP) is 9.3 kb.

以绵羊β乳球蛋白基因（β-Lactoglobulin，β-LG）为转基因表达框架，将人G-CSF(Human Granulocyte Colony Stimulating Factor， hG-CSF)基因与报告基因-增强型绿色荧光蛋白(Enhanced Green Fluorescenct Protein， EGFP)基因作为双表达单元拼接到β-LG基因的第一外显子处，并在G-CSF基因两侧引入同源重组位点loxP、lox2272，将打靶基因表达构件β-LG-hG-CSF-IRES-EGFP(总长9.3 kb)分为两段进行构建，片段Ⅰ（长：5.9kb）与片段Ⅱ（长5.6 kb）两段重叠部分为2.2 kb。

A Gene Reference Into Function is a concise phrase describing a function of a gene in the Entrez Gene database.

Gene Reference Into Function是一个描述Entrez基因数据库中一个基因的一个功能的简明的短语。

Reverse transcription polymerase chain reaction and western blot were used to detect the COCH and POU3F4/brn4 gene's expression. To investigate in vitro characteristics of COCH gene's expession, two factors were introduced in the culture medium of spiral ligament type Ⅰ fibrocytes respectively. Firstly, phosphorothionate-modified antisense oligodeoxynucleiotides to POU3F4/brn4 mRNA was applied to study the regulation effects of POU3F4/brn4 to COCH gene transcription.

引入人工合成的针对POU3F4／brn4基因mRNA的反义硫代磷酸寡聚脱氧核苷酸，干扰耳蜗螺旋韧带Ⅰ型成纤维细胞POU3F4／brn4的表达，免疫印迹检测反义干扰细胞POU3F4／brn4蛋白条带的强弱，RT-PCR检测反义寡核苷酸干扰细胞COCH基因转录水平的变化，观察POU3F4／brn4对COCH基因的转录调控作用。

The nucleotides sequence and deduced amino acid sequence of these cDNA figments mentioned above were divided into two fractions. One is carbon part of L gene , the other is 5 terminal,and they were compared with other rabies-related virus strains published in NCBI previously.The result show that the homology of nucleotide sequence and amino acid sequence of carbon part in L gene among these two rabies virus strains is 91.3% and 95.8%, and these two strains belong to type of gene I.

本实验以吉林鹿源DRV8202株及河南鼠源野毒株MRV的L基因的C端为主要研究内容，用RT-PCR和3'-RACE方法对其进行了克隆和测序，将所测得的核苷酸序列和推导的氨基酸序列分成两个部分，一是L基因C端序列，另一个是L基因5'端的非编码区，并与NCBI上公开发表的狂犬病毒其它毒株的相应片段进行分析比较。

The phytase gene was cloned in this study would not only provided excellent gene resource which could express in the microbe and plant but also remedied the vacancy of phytase gene cloned in the funguses.

本研究所克隆的植酸酶基因不仅为植酸酶进一步在各种微生物、植物中表达提供了优良的基因源，而且弥补了食用菌中克隆植酸酶基因的空白。

And function, genomes, gene expression, control of gene expression and gene engineering.

分子生物学是生命科学体系中的一门重要学科，也是高等医药教育必需学习的课程之一。

The cells in cross-section of leaf and stem become swollen and intercellular space of the ground tissue increass when the expression of UDPGDH gene was inhibited in tobacco. When UDPGDH gene was over expresed, opposite physiological changes were observed. These results further suggested that UDPGDH gene may play an important role in the synthesis of pectin and hemicelluloses in ramie.

初步的组织学分析表明，反义和干扰表达的转基因植株中，茎段与叶横切面细胞结构较为松散，基本组织细胞间隙增大，而在UDPGDH基因过量表达的转基因植株中则观察到相反的现象，进一步证实UDPGDH基因在苎麻半纤维素和果胶合成中所具有的重要作用。

Warden: That's a beautiful dog, but don't bring her again.

那是条漂亮的狗，但是不要再把她带来了。

You get up in the morning – no hangover!

你第二天早上起来的时候—不会宿醉！