查询词典 modifier gene

The genetic study suggested that the carbendazim-resistance in these mutants could be steadily inherited by asexual and selfed reproduction, and the resistance was controlled by the same single major gene both in laboratory mutants and field resistant isolates, the different levels of resistance or the same resistant level in different strains maybe conferred by mutations at different sites or one site with different allelic mutations. The gene compensates for the sensitivity to MDPC was allelic to that governs the carbendazim resistance, but the mutation for increasing sensitivity to MDPC in this gene could get the pathogen highly resistant to carbendazim.

抗药性遗传研究表明，在所研究的抗药突变体中，抗药性在自交和无性繁殖后代中能稳定遗传；室內抗药突变体和田间抗药菌株对多菌灵的抗药性由同一个主效基因控制，但它们发生突变的位点不同或者同一碱基位点发生了不同的突变；对MDPC的敏感性也是由单个基因控制的，该基因与控制多菌灵抗性的基因是等位基因，当该基因发生对MDPC的敏感性增加的突变时会使病菌对多菌灵产生高水平抗性。

In order to elucidate the relationship between the structural features of leginsulin gene in legume plants and their phylogenetic significance,we have cloned the cDNA sequence of leginsulin gene from radicles of broad bean via RT-PCR techniques according to the leginsulin gene sequence we previously obtained from soybean.The cloned cDNA encoded for a precursor protein consisting of the signal peptide,mature leginsulin and an additional 45 amino acids of another polypeptide.

为探明豆科植物中豆类胰岛素基因的结构特征与进化关系，在已获得大豆豆类胰岛素基因的基础上，以蚕豆种子胚根mRNA为材料，采用RT-PCR技术，克隆了蚕豆豆类胰岛素基因的cDNA序列，编码的前体多肽包括信号肽、成熟型豆类胰岛素及另一多肽的45个氨基酸残基。

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

以牛泡沫病毒(Bovine foamy virus， BFV)中国株BFV3026原病毒DNA为材料，构建R区系列缺失质粒，通过对其转染细胞中RT水平及对缺失质粒与luc报告质粒共转染细胞中萤火虫荧光素酶活性的测定，确立U5区对于BFV3026两类启动子LTR和IP均具有负调控作用；同时将带有不同R区的BFV3026结构基因片段克隆于异源启动子CMV之下，通过对其转染细胞293T中RT酶活性的测定，确立R区对于病毒结构基因pol的表达具有一定的调节作用，并将其功能区域初步界定在R区5′端100bp内。

Besides characterisation of the two genes' function, RT-PCR, Northern analysis, primer extension, and lacZ reporter gene fusions were also tried in this study to investigate the genetic organization of the operons related and their transcription regulation, map gene was found to locate at the end of a long ribosome protein genes operon and yflG is in another operon with its downstream gene.

除了对这两个基因的功能鉴定，本文还阐明了它们各自所在操纵子的遗传结构，并采用RT-PCR、Northern印迹及引物延伸等多种方法，最终分别对它们的启动子进行了定位。

Coli lacZ gene at icp34.5 gene (RL1), an antiapoptosis and neurovirulence gene, which was constructed in our lab, is one of the oncolytic viruses.

本室构建的缺失凋亡抑制基因icp34.5并插入LacZ基因的单纯疱疹病毒就是一种有靶向性的溶瘤病毒。

To observe whether the re-expression of NAG7 gene could affect the gene expression of HNE1 cells, the cDNA microarray technique was employed to analyze the changes of gene expressions.

同时，运用cDNA微阵列技术分析NAG7基因重表达对HNE1细胞基因表达谱的影响。

Gene drugs ; gene therapy ; gene vaccine ; antisense RNA ; triplex-forming DNA

基因药物；基因治疗；基因疫苗；反义RNA ；三链DNA

Used the method of touch down PCR, the ACS2 gene was cloned from DNA of Petunia hybrida. At the same time, its gene sequence DQ090003 was obtained in GenBank, the antisense repetition of ACS2 linked the plasmid pBI-121 by use of one step. In the result, the expression vector having antisense repetition gene was constucted.

利用降落PCR的方法，从矮牵牛DNA中扩增出ACS2基因片段，在GenBank中获得基因序列号为DQ090003；利用一步法将ACS2基因的反义重复与pBI-121质粒连接，构建含该基因的反义重复表达载体。

FHIT gene alterations preferentially occur in squamous cell carcinoma patients and smokers, and FHIT gene may be a candidate molecular target of carcinogenesis in tobacco smoker. Allelic deletion of FHIT gene might be an early molecular event in smoking-related lung cancer.

FHIT等位基因缺失主要发生在肺鳞癌和吸烟患者中，且FHIT基因可能为烟草致肺癌的靶基因，其等位基因缺失可能是肺癌的早期分子事件。

Lines transformed by particle bombardment have been discussed. The selectable marker bar gene cassette that integrated with the rice genome had multiple copies and showed complex segregation behavsensitive plants in their progenies. In five out of ten original transgenic lines, bar gene can be stably transmitted as a dominant gene to self-pollinated T2 progeny.

结果发现：作为筛选标记的bar基因表达盒在水稻基因组中多拷贝整合，遗传分离行为复杂，还出现了Basta抗感分离比在35∶1～144∶1之间的&假纯合体&，但50﹪转基因株系中(5/10)bar基因可作为一个显性基因按孟德尔方式稳定遗传至自交T2代。

I can not negligent, he should do what?

我不能不在意了，他要做什么呢？

It is very dangerous to swim in the river alone.

一个人到河里游泳是很危险的。