查询词典 modifier gene

The results showed that there were differences among treatments in transformation of high quality gene(Glu\|D 1 5+10) in F 1 grains, and heterozygous type was more frequently appeared in the treatment of irradiation. In the F 2 segregative population, heading date deviated to early\|mature parent in the treatment of reciperocal cross and its combination with irradiation, indicating that cytoplasm and irradiation could promote the heading. In F 3 the expressive frequency of (5+10) gene in the cross was lower than that in reciperocal cross, and irradiation treatment could raise the expressive frequency of(5+10) gene. These results showed varience in different crosses.

结果表明，不同组合处理间F1籽粒优质亚基(Glu D15+1 0 )的转化存在差异，辐射处理的多以杂合型出现。F2 分离群体中，反交及反交结合辐射处理的抽穗期明显倾向于早熟的亲本，细胞质与辐射具有促进抽穗期的作用。F3 植株Glu D15+1 0亚基的表达频率，不论是哪种组合，反交时 5+1 0亚基的表达频率高于正交；无论正交还是反交，辐射处理提高了 5+1 0亚基的表达频率，而且在不同组合间存在差异。

The N gene protein was expressed in the CHO cells proved by IFA. The N gene was transcripted as proved with RT-PCR. CHO/CDV-N cell strain was constructed, which provided evidence for research of CDV serumal detection and gene vaccine.

然后通过磷酸钙共沉淀法转染CHO-K1细胞，通过潮霉素筛选得到阳性克隆，间接免疫荧光实验鉴定N基因在CHO细胞中的表达，并用RT-PCR方法从转录水平证实N基因在CHO-K1细胞中的表达，最终建立了CHO/ CDV-N细胞株，为犬瘟热病毒的血清学检测和基因疫苗的研制奠定了基础。

Sequence analysis and homology alignment showed that MD1 had 93% similarity with matK in maize chloroplast genome, a gene encoding maturase included in type Ⅱ intron splicing of RNA transcript; MD2 had 99% similarity with gene PP2C, encoding serine / threonine protein phosphatase type 2C in extremely drought tolerant Sporobolus stapfianus; and MD3 had 99% similarity with rice gene encoding metacaspase, belonging to aspartic acid specific cysteine caspases.

序列分析和同源性比对表明，MD1与玉米叶绿体基因组中编码参与RNA转录本Ⅱ型内含子剪切的成熟酶的matK基因有93%的相似性，MD2与极端耐旱的Sporobolus stapfianus的丝氨酸／苏氨酸2C型蛋白磷酸酶基因PP2C的相似性达99%，MD3与属天冬氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase基因有99%的相似性。

Results of sequence and homology analysis show that MD 1 has 97% similarity with mark in maize chloroplast genome,a gene encoding RNA maturase involved in group Ⅱ intron splicing of RNA transcript;MD2 has 99% similarity with the gene serine/threonine phosphorylase 2C in Sporobolus stapfianus;and MD3 has 99% similarity with rice the gene encoding metacaspase,an arginine/lysine-specific cysteine protease.

序列分析和同源性比对表明，MD1与编码成熟酶的玉米叶绿体基因matK有97%的相似性，MD2与极端耐旱植物Sporobolus stapfianus编码丝氨酸/苏氨酸蛋白磷酸酶的PP2C基因有99%的相似性，MD3与属精氨酸/赖氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase酶基因有99%的相似性。根据MD2片段序列，结合电子克隆和RT-PCR方法，克隆出一条1731 bp的全长cDNA序列，它编码388个氨基酸。

To obtain the tervalent fusion toxin gene, three toxin gene fragments from three species of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio mimicus were connected with the flexible linker using overlap extension PCR. The three toxin gene fragments respectively encode the mature proteins of the thermostable direct hemolysin of V. parahaemolyticus, the cytotoxin of V. vulnificus and the heat-labile hemolysin of V.

采用柔性Linker和重叠延伸PCR方法将三个毒素基因，即副溶血性弧菌的耐热型直接溶血素基因tdh、创伤弧菌的溶细胞毒素基因vvhA和拟态弧菌的热不稳定型溶血素基因vmhA的成熟蛋白基因片段进行串联，得到三联融合毒素基因tdh-vvhA-vmhA。

The research demonstrated that the sperm-mediated gene transfer method (direct injection of copulation thecae) can introduce foreign gene into silkworms, and that the foreign gene may be integrated in the chromosomes of silkworms with many copies in series and can be transmitted to G1 offspring.

本研究的结果证明了精于介导基因转移方法能将外源基因有效地导入并整合于家蚕基因组，导入的外源基因可能是以串联多拷贝的形式整合于家蚕基因组中，整合的外源基囚叶＠传午G；代。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

The paper aimed at the research status of main transgenic techniques in wheat and transferring the transeunt gene into wheat , the content include researches of the quality gene , resistant herbicide gene and other important genes transferred into the wheat with all kinds of methods , especially the way of particle bombardment .

其内容包括几种主要的小麦转基因方法和以基因枪法为主的各种转化技术对品质基因，抗除草剂基因和抗病等基因在小麦中的遗传转化研究进展

A strain NK13 was screened for a certain extent asymmetric hydrolyze the rac-ketoprofen Chloroethyl ester and identified as Bacillus megaterium. For the preparation of gene libraries, a positive clones was obtained from the tributyrin flat. The sequence of this esterase gene had been analysised, and contained the whole ORF of an esterase gene with the length of 933bp.

以本实验室筛选出的一株具有不对称拆分消旋酮基布洛芬氯乙酯的菌株NK13为材料，经初步鉴定为巨大芽孢杆菌，通过构建其基因文库，从中筛选得到一阳性克隆重组子pUC-NK。

Results The mecA gene and tst gene were detected,and were made the gene sequencing successfully.

结果 成功的对金黄葡萄球菌mecA基因和tst基因进行了检测，并进行基因测序。

Warden: That's a beautiful dog, but don't bring her again.

那是条漂亮的狗，但是不要再把她带来了。

You get up in the morning – no hangover!

你第二天早上起来的时候—不会宿醉！