查询词典 modifier gene

Methods The 26th exon, the 30th exon and the 21st intron of gene GP Ⅱb in 110 individuals were amplified by polymorphism and sequenced to investigate whether there was linkage among the polymorphisms of the gene. Human platelet antigen-3 (HPA-3) gene frequency was detected by Fok 1 enzyme in 147 patients with hematologic diseases, and was compared with that in 110 normal individuals. Forty-four patients who received apheresis platelet transfusion repeatedly were randomly divided into the HPA-3 homotype group and the control group. The antibodies of the platelet were detected after 3 times of platelet transfusion.

聚合酶链反应－单链构型多态性分析检测110例正常人GP Ⅱb基因第26和30外显子(Exon 26， Exon 30)及21内含子(Intron21)基因多态性，进行基因序分析，研究这些基因多态性是否存在连锁关系；应用Fok1酶切法对147例血液病人人类血小板抗原－3(human platelet antigen-3， HPA-3)基因进行分型，并与110例正常人进行比较；将接受单采血小板输注的44例血液病人随机分为HPA-3同型输注组和对照组，血小板输注3次以后检则血小板抗体。

This study intends to investigate the correlation between HBV-GN and HLA-DR allele by testing the frequency of HLA-DR gene in HBV-GN patients, to probe into the effect of immunogenetic factor on the pathogenic mechanism of HBV-GN at the genic level and further explore susceptibility gene and/or resistance gene associated with HBV-GN disease, so as to provide certain experimental basis for diagnosis and treatment of HBV-GN.

本研究拟通过检测HBV-GN患者的HLA-DR的基因频率，对HBV-GN与HLA-DR等位基因相关性进行研究，从基因水平探讨免疫遗传因素在HBV-GN发病机制中的作用，并进一步探索HBV-GN疾病相关的易感基因和/或抗性基因，从而为HBV-GN的诊断和治疗提供一定的实验依据。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

The main component include promoter sequence (5' flank control rigon of goat P - lacto globulin gene of goat),foreign gene,Marker gene and genome of defective retrovirus.

其中主要构建元件包括：启动子序列(山羊β-乳球蛋白基因5'端调控区)；外源基因(人乳铁蛋白基因cDNA)；标记基因；缺陷型逆转录病毒基因组。

The further study will identify the defected gene of the lamellar cataract, and make it possible for gene diagnosis and gene therapy.

进一步的研究工作将明确其致病基因，使该病的基因诊断和基因治疗成为可能。

Signal peptide sequence was removed lest it couldnt be recognized by Bacillus subtilis, gene coding for mature peptide was ligated to downstream of sacB signal peptide sequence of pWB980 to form a new ORF and generate pWB980-LipA, gene coding for its chaperone was also amplified and ligated to the downstream of LipA to generate a secretion/expression vector pWB980-LipAB.thechaperone gene was sequenced and analyzed by multiple alignments, resulted showed that there were mutations of nucleotides(1～7) and amino acids (1～2) with the other reported genes.

为了防止枯草芽孢杆菌信号肽酶不能识别脂肪酶的信号肽，切除掉脂肪酶的信号肽编码序列，与枯草芽孢杆菌分泌表达质粒pWB980连接并与质粒上的SacB信号肽构成一个完整的开放式阅读框，获得重组质粒pWB980-LipA，因为该脂肪酶的活性表达需要一个特异性分子伴侣帮助折叠成有活性的构象，将脂肪酶分子伴侣基因串连到脂肪酶基因的下游，获得重组分泌表达载体pWB980-LipAB并且测序分析分子伴侣的基因序列和氨基酸序列并与其它报导的序列进行了比对，发现有1到7个碱基的差异和1到2个氨基酸残基的突变。

The expression of cloned cold-adapted lipase gene lipA in Pichia pastoris GS115 with the pPIC9k as the expressive vector was performed according to the methods described in the manual, version 3.0, of the pPIC9K identified the lip gene cloned here was a functional lipase gene.

以pPIC9K为表达载体，以GS115为表达宿主菌，成功构建重组表达质粒pPIC9K-lipA，经甲醇诱导表达，能够分泌有脂肪酶活性的表达产物，因此证明本研究所克隆的脂肪酶基因lipA确实为脂肪酶开放阅读框。

It was then introduced by gene pulser into acrystalliferous strain CryB and a recombinant strain CryB (pBMBZGC10) was obtained. Different fermentative solutions of recombinant strain were used for multi-spray on leaves of Brassica pekinesis, Ipomoea aquatica and Lycopersicon esculentum. Results by using fluorescent detection and PCR amplification revealed that cry1Ac10 gene could not been detected in roots, stems and leaves of plants tested. The cry1Ac10 gene was also not transferred into indigenous bacteria, actinomyces and fungi in soil.

苏云金芽胞杆菌标记重组菌株与杀虫基因水平转移的研究将重组菌株CryB（pBMBZGC10）的发酵液按浓度梯度分次进行喷洒小白菜、蕹菜和番茄作为供试植株，利用标记基因研究的结果表明：cry1Ac10基因没有向供试土壤细菌、真菌和放线菌转移，也未在植物的供试植物根、茎和叶中检测到该基因。

The DNA sequences around the transposon inserted site were also amplified by inverse PCR and sequenced. Multisequence alignments showed that the mutated genes were highly similar to the malonyl coenzyme A transacylaseg gene, lipopeptide synthetase B gene and sensor histidine kinase gene, respectively.

反向PCR克隆转座插入位点周边序列及测序结果表明，转座突变的基因与编码脂肽类化合物合成和调控相关的malonyl coenzyme A transacylase、lipopeptide synthetase B和sensor histidine kinase蛋白基因具有高度同源性。

Objective: The genes were connected basing on obtain gene of MCP and DAF of liver and fetal marrow of Chinese adult and then design mammiferous vector of the hMCP-DAF fusion gene. Finally transfer hMCP-DAF fusion gene to the early embryo of jinhua''s pig.

目的 在获得中国成人肝脏、胎儿骨髓MCP、DAF基因克隆的基础上，进一步将两个基因串接，并构建hMCP-DAF融合基因的哺乳动物表达载体，筛选出具表达活性的hMCP-DAF并导入金华猪早期胚胎。

Cable dampener cannot be rolled up but must be hung over the cable.

缆帆不能被卷起，必须保证处于展开状态

"This skylark is a present from my master," he says.

"这只云雀是我主人献上的礼物。"他说。