查询词典 modifier gene

SMMC-7721 cells transfected with antisense DNMT1 (DNA methyltransferase Ⅰ) gene fragment (SMMC-7721 aDNMT1) was more sensitive to TRAIL than SMMC-7721 cells transfected with sense DNMT1 gene fragment or vectors without any ectogenous gene fragment.

4MTT法和TUNEL法测得的结果显示，肝癌SMMC-7721细胞转入反义DNMT1基因片段后对TRAIL更加敏感。

There were few studies on the causative gene of PCOS in our country. We chose the candidate gene associated with androgen receptor gene to study, considering the endocrinal characteristics such as hyperandrogenism.

本课题针对PCOS常见的高雄激素血症的内分泌特征，选择雄激素受体基因进行研究，旨在探讨AR基因微卫星CAG多态性与PCOS发病及临床特征的关系，寻找PCOS的致病基因，以期为PCOS的预防和治疗提供理论基础。

Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具，分别以人丁酰胆碱酯酶(1p0i：A)和果蝇乙酰胆碱酯酶(1d×4：A)的蛋白晶体结构为模板，对嗜虫书虱2个AChE的三维结构进行同源建模，并在三维结构中发现了AChE的酶解活性位点，证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少，在GenBank中没有可用作内参基因的序列，因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号：FJ041117)，该片段长度为822 bp，编码273个氨基酸残基，同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

ASR1 is a gene from early auxin-responsive GH3 gene family, and the protein encoded by this gene is overexpressed in the mutant, which results in the following morphological phenotypes including dwarf, epinastic rosette leaves, and restrained lateral root formation, etc. Meanwhile, ASR1 may modify the plant defense signaling molecule salicylic acid in vivo, and therefore asr1 mutant is more susceptible to the infection from the pathogen Pseudomonas syringae.

ASR1属于生长素相关的GH3基因家族，它编码的蛋白质在突变体内过量表达，并造成植株矮化，莲座叶片向下卷曲，侧根发生受到抑制等形态表型的改变，同时，ASR1可能还参与了植物体对抗病信号分子水杨酸的调控或修饰过程，并使asr1突变体对病原菌丁香假单胞杆菌的侵染更敏感。

To investigate the role of gene Gli3 in idiopathic congenital talipes equinovarus, we constructed the Gli3 luciferase reporter gene expression vectors to analyze the promoter activity of the rat gene Gli3. The regulatory element in the promoter region of the rat Gli3 was predicted using P-Match software and further verified by ChIP experiment.

为了探讨人类GLI3基因在单纯性马蹄内翻足(Idiopathic congenital talipes equinovarus， ICTEV)发生时所起的作用，文章构建了大鼠Gli3基因启动子区域荧光素酶报告基因表达载体来分析Gli3基因启动子的活性，并利用P-Match软件预测大鼠Gli3基因启动子区域可能的调控元件，应用ChIP实验加以验证。

Methods: the experimental condition for preparing the gene chips was studied and 7 fragments were screened from GenBank as probes dotted on chips to make HBV genotyping gene chips, many examinatorial experiments were done to evaluate the quality of this gene chip, and to analysis its detection characteristics.

对大量HBV各基因型序列对比分析后，设计7支探针，用芯片点样机点样于经预处理的玻片上制成HBV基因型检测芯片；对芯片检测的实验条件进行研究探索；进行芯片质量性能的考察实验，分析其检测特性。

ERAF17 gene expresses differently between male and female reproductive organs. Its expression is induced by ethylene or ethylene released compounds that can promote femaleness. No evidence directly shows the relationship between ERAF17 gene and femaleness, however. So this work focus on proving the positive correlation between the expression of ERAF17 gene induced by ethylene and development of female flowers,then search for the signal pathway of cucumber female reproductive organ development.

ERAF17基因在黄瓜的雌雄生殖器官的表达不同，可以由乙烯诱导表达，从而推动雌性化，但是它的作用机制却不清楚，并没有明确的证据表明ERAF17基因与雌花的发育有直接关系，所以本论文研究重点在于证明乙烯诱导ERAF17基因的表达对黄瓜雌花的分化有确定的正相关，从而可以探索黄瓜雌性器官发育存在的可能的信号通路，并对应用前景进行评估。

Coli and S.aureus , the experiment showed that recombined gene peptide has the antibacterialactivities.Conclusion The recombined gene antibacterial peptide was expressed successfully in the fusional pTYB vetor and the fusion gene peptide CA(1 ~8-MA(l ~12),CAM has the antibacterial activities to E. coli and S.aurens. While how to frame a more efficient expression system should be studied further , and its other biological activities are still to be tested.

本实验通过基因克隆方法拼接重组基因抗菌肽与融合表达载体pTYB12并在大肠杆菌中进行表达成功，抑菌活性的检测结果表明重抗菌肤的克隆表达与活性初步测定中文摘要组基因抗菌肤CA(1一8卜MA(1一12)，cAM〕对大肠杆菌与金黄色葡萄球菌均有一定的抗菌活性，如何进一步提高抗菌肤的表达量和有关重组基因抗菌肤的生物学活性还有待于进一步研究。

One, the progress of the research of mankind of gene medicines and chemical reagents to oneself gene and gene project will be in will make pharmacy line of business obtains a leap in 10 years henceforth, remedial osteoporosis, early old sex fiber of sex of gawkish, bursa is out of shape the medicaments that waits for difficulty miscellaneous disease will come out, the cure of AIDS also will obtain a breakthrough.

一、基因药品人类对自身基因的研究和基因工程的进展将在今后10年中使制药业取得飞跃，治疗骨质疏松、早老性痴呆、囊性纤维变形等疑难杂症的药物将问世，艾滋病的治疗也将取得突破。

The wheat germin gene was cloned by PCR and a plant expression vector was constructed harboring the germin gene drived by CaMV 35S promoter. Germin gene has been transferred into the tabacco plants.

本研究通过PCR扩增克隆了小麦germin基因，构建了35S启动子驱动的germin基因植物表达载体，经遗传转化获得了转基因烟草植株。

Cable dampener cannot be rolled up but must be hung over the cable.

缆帆不能被卷起，必须保证处于展开状态

"This skylark is a present from my master," he says.

"这只云雀是我主人献上的礼物。"他说。