查询词典 modifier gene

The cloned BBE gene sequences were 94.84% identified with the reported BBE genes in GenBank previously. Based on the cDNA sequences of COR and BBE ,two fragments about 400～500 bp from each gene with lower identity among them were cloned. The fusion gene BC (744 bp) is fused by the PCR technique. Then the promoter CaMV 35S driven, containing'forward BC fusion fragments-reverse pdk intron-reverse BC fusion fragments', plant siRNA expression vector were constructed based on the vectors pHANNIBAL and pART27.The work will lay the foundation for breeding a low morphine and high thebaine poppy.

利用blast及分子生物学软件DNAStar对COR和BBE基因的cDNA序列同源性进行分析比较，分别从各基因中筛选和克隆了一段同源性极低、约400～500 bp的片段；并应用重叠PCR法将其拼接成744 bp的融合基因BC，以中间载体pHANNIBAL和植物表达载体pART27为基础，构建了以CaMV 35S启动子驱动的含有"正向BC融合片段- pdk内含子-反向BC融合片段"的ihRNAi植物表达载体，通过转化野生罂粟，初步研究了以COR和BBE基因为靶标的RNAi对内源吗啡合成的抑制效果，为进一步培育低吗啡高蒂巴因的罂粟种质提供了依据。

Tube question grows which for the solution above standard, this research take mobile phone industry as a background, biological gene foreword generally unifies with the product tube, and the coordinate gene foreword compares to develops algorithm Basic local alignment search tool as well as transposed matrix Block substitution matrix the application, constructs the product gene tube system overhead construction, provides one same angle product tube way.

为解决上述色规范所衍生出的管问题，本研究以行动电话产业为背景，将生物基因序的概与产品管相结合，透过「产品特性」、「客户特性」、「订单特性」以及「色特性」等四大虚拟基因的定义，并配合基因序比对演算法 Basic local alignment search tool 以及置换矩阵 Block substitution matrix的应用，建产品基因管系统架构，提供一种同角的产品管方式。

Using semi-quantitative RT-PCR, the differential expression profiles of eleven selected genes were confirmed in the ovaries of triploid and diploid. These genes fell in gene categories with a wide range of functions. The results indicated that triploidy affects the dynamic gene regulatory network in triploid ovary. This study established a firm basis for future investigation on characterization of crucial molecular events for normal ovarian development in shrimp.To further dissect exact gene functions for gonad development of shrimp, three differentially expressed genes between diploid and triploid ovary, PCNA (proliferating cell nuclear antigen), CAS/CSE1 (cellular apoptosis susceptibility protein/chromosome segregation 1) and SSRF (spermatogonial stem-cell renewal factor) were characterized on certain aspects.

利用抑制性消减杂交技术，建立了对虾二倍体和三倍体卵巢间的2个消减文库；在正向消减文库(以三倍体卵巢作为实验组，二倍体卵巢作为驱动组)中，鉴定到54个基因；在反向消减文库(以二倍体卵巢为实验组，三倍体卵巢为驱动组)中，鉴定到16个基因；选取11个差异表达的基因，利用半定量RT-PCR的方法对其在二倍体和三倍体卵巢间的表达进行了检测，均能很好地与消减结果相吻合；这些差异基因编码多种功能的蛋白，分析表明染色体的三倍化使三倍体卵巢中的基因调控网络受到了影响；为深入揭示维持卵巢正常发育的关键分子调控事件奠定了基础。

Methods Amplify the cDNA sequence encoding truncated HCV gene, with a part of carboxyl-terminus deleted, by PCR. Synthesize the mimic epitope at E2 region of HCV and seven T or Th cell epitope genes of NS3-NS5 respectively. Bind HCV core gene with a part of carboxyl-terminus deleted to the synthetic epitope gene by PCR, then clone into eukaryotic expression vector pcDNA3.1 and transiently transfect COS7 cells.

用PCR方法扩增核心区羧基端部分缺失的基因片段；分别合成HCV E2区模拟表位和NS3~NS5 7个T或Th细胞表位基因；PCR方法将羧基端部分缺失的HCV核心区基因与合成的表位基因串联，克隆入真核表达载体pcDNA3.1，并通过脂质体瞬时转染COS7细胞。

Acquisitions of 2001 2001 年的购并案 A few days before last year's annual meeting, I received a heavy package from St. Louis, containing an unprepossessing chunk of metal whose function I couldn't imagine. There was a letter in the package, though, from Gene Toombs, CEO of a company called MiTek. He explained that MiTek is the world's leading producer of this thing I'd received, a "connector plate," which is used in making roofing trusses. Gene also said that the U.K. parent of MiTek wished to sell the company and that Berkshire seemed to him the ideal buyer. Liking the sound of his letter, I gave Gene a call. It took me only a minute to realize that he was our kind of manager and MiTek our kind of business. We made a cash offer to the U.K. owner and before long had a deal.

就在去年股东会的前几天，我收到从圣路易寄来的一个大包裹，里面装了一块看不出做何用途的金属，包裹里还有一封信，署名 Gene Toombs-他是一家叫做 MiTek 公司的总裁，他解释说 MiTek 是专门制造这玩意儿的世界级领导厂商，也就是用来做屋梁的连接板，他进一步表示 MiTek 的英国母公司有意出售这家公司，他认为 Berkshire 应该是最合适的买家，我相当欣赏他写这封信的语气，所以当下打电话给他，几分钟的谈话，我就发觉他是属于我们类型的经理人，而 MiTek 也是我们想要的公司类型，于是我们报了一个价给其英国母公司，没多久后就正式成交。

Rats living over 96h were considered survival. To observe the protective effects of ALR gene on acute liver injury rat, thirty-six rats that were injected peritoneally with 50% CCl4 of 2ml/kg were divided into the flowing 6 group according to doses (50?g/kg and 200?g/kg ) and administration routes of pcDNA3-ALR DNA 4h after CCl4 injection: group 1, model group; group 2, ALR gene of 50μg/kg was injected venally; group 3, ALR gene of 200?

另取36只大鼠按2ml/kg腹腔注射50% CCL4，染毒后4h按不同剂量（50μg/kg 和200μg/kg ）、不同注射途径（尾静脉注射、腹腔注射和联合注射）随机分为六组。1组：模型组，不注射ALR基因； 2组：尾静脉注射ALR基因 50μg/kg； 3组：尾静脉注射ALR基因 200μg/kg； 4组：腹腔注射ALR基因 50μg/kg； 5组：腹腔注射ALR基因 200μg/kg； 6组：联合注射（尾静脉和腹腔各注射ALR 基因100μg/kg）。

The invention discloses a pyruvate oxidase gene derived from viridian aerococcus, recombinant expression plasmid constructed by the gene and an engineering strain of escherichia coli gene transformed by the recombinant expression plasmid.

本发明公开了一种绿色气球菌来源的丙酮酸氧化酶基因AvPyOD，该基因所构建的重组表达质粒，以及该重组表达质粒转化的大肠杆菌基因工程菌株。

The method is simple, has safe operation, creates an approach utilizing a plant gene to safely select the marker gene for the tomato genetic transformation, can replace the disadvantages caused by the widely used heterogenous genes such as antibiotic genes, weedicide resistant genes, xyl A, pmi, GUS and so on, taken as marker genes, obtains a transgenic tomato with food safety, biological safety and ecological safety, relieves the public from anxiety of marker gene safety, and inevitably facilitates the commercialized application of the transgenic tomato.

该方法简单，操作安全，开创了利用植物基因作为番茄转化安全筛选标记基因的途径，能够代替目前广泛使用的抗生素基因、抗除草剂基因以及xylA、pmi、GUS等异源基因作为标记基因存在的弊端，获得具有食品安全性、生物安全性和生态安全性的转基因番茄，解除公众对标记基因安全性的顾虑，必将促进转基因番茄的商业化应用。

METHODS: MyoD cDNA fragments were extracted from plasmids pEMSV-MyoD with polymerase chain reaction, and PCR was used to clone the whole-length gene of MyoD. After adding CACC sequence at 5' end, MyoD gene was cloned by orient topology into transfer ventor, pENTR/D-TOPO. Objective gene was transferred into adenoviral expression vector DNA via pENTR/D-TOPO vector. The recombinant adenoviral vectors transfected into HEK293A cells by using lipofectamine were packaged and amplified.

从pEMSV-MyoD质粒上用聚合酶链反应法扩增出MyoD cDNA片段，再通过聚合酶链反应使MyoD基因加上CACC序列接头，经过定向拓扑克隆使目的基因连接到转移载体上，再通过LR酶促反应，将目的基因转移到腺病毒表达载体DNA上，获得MyoD基因重组的腺病毒DNA，用脂质体转染法转染HEK293A细胞，包装扩增出MyoD基因重组的腺病毒。

LacZ gene was fused with the promoter of the acoR gene and bkdR gene, and two recombined genes were expressed in sigL mutant strain and HD-73 wild strain sequentially.

分别将调控aco操纵子（编码3-羟基丁酮脱氢酶系统）的转录调节基因acoR和调控bkd操纵子（编码催化支链脂肪酸合成的酶系统）的转录调节基因bkdR的启动子与lacZ基因融合，并转入出发菌株和sigL突变体中，测定b-半乳糖苷酶的活性。

But GST= 0.156, Nm=1. 588. As a result, the foundation of Youyongchi Avicennia marina population was the result of the migration of hypocotyles and human factors.

这项工作可以为海岸防护林中新引进种类的判定以及为研究种群建立者效应方法的确定提供科学依据。

The two-dimensional CDRC-ADI-FDTD update equations for collision unmagnetized plasma are induced. The unconditional stability of the CDRC-ADI-FDTD formulation for collision unmagnetized plasma is obtained by the examples.

推导了碰撞非磁化等离子体中的二维CDRC-ADI-FDTD迭代公式，并用算例验证了碰撞非磁化等离子体CDRC-ADI-FDTD算法也是无条件稳定的。