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To get more information of the function of human Era on cell cycle, the complete control inducible mammalian expression system was used to express human Era mutant and analyzed cell cycle of uninduced (add 1% ethanol) and induced (10μM Pon A) cell lines by flowcytometer, the primary results demonstrated human Era mutant could inhibit apoptosis induced by ethanol, and Era S36N may stimulate the expression of Bcl-2 as detected by Western Blot.

在构建稳定高表达蜕皮激素受体基因的细胞株3V3的基础上，转染突变型人Era的表达载体进行压力筛选，对所得到的单克隆细胞株进行诱导与未诱导条件下细胞周期的分析，结果表明未诱导加酒精的对照细胞可以引起细胞凋亡，而Era诱导表达后可以显著抑制凋亡细胞的数量，Western表明Era S36N可以诱导细胞Bcl-2表达，提示人Era可能与细胞凋亡相关。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Here were expression of TGFαand EGFR mRNA in unmatured oocyte, matured oocyte, embryo and the expression of TGFαand EGFR in matured oocytes were comparatively more abundant than that in unmatured oocytes.

GFαmRNA的量在8细胞期下降到最低水平，并随着胚胎基因组的激活胚胎开始转录TGFα，使TGFαmRNA表达总量保持在一定水平，持续到桑椹胚期；EGFR mRNA的量在胚胎发育到桑椹胚期下降到最低，而囊胚期胚胎中的整体表达量又上升。

RESULTS: APC promoter 1A was methylated in NCI-H460 cells, and unmethylated in NCI-H446 and SPC-A1 cells. Hypermethylation was detected in all 5 CpG islands (687, 707, 714, 719, 726) of APC promoter 1A in NCI-H460 cells. The expression of APC in NCI-H460 cells was decreased by 26.04% of that in NCI-H446 cells and by 32.36% of that in SPCA1 cells. After treatment of 1, 5, 10, 15μmol/L 5-aza-dC, the expression of APC promoter 1A in NCI-H460 cells was enhanced by 4.59, 5.78, 9.58, 5.98 folds, respectively.

结果：SPC-A1和NCI-H446细胞APC甲基化阴性，NCI-H460细胞APC甲基化阳性；甲基化芯片检测Ncl-H460细胞在APC启动子1A 5个CpG位点均存在甲基化(687、707、714、719、726)，SPC-A1和NCI-H446甲基化阴性，荧光定量结果NCI-H460的APC转录较SPC-A1和NCI-H446有明显的下降，仅为二者平均的30.04%；经5-aza-dC脱甲基化作用后，NCI-H460细胞的APC表达增加了约5~10倍，其中10μmol/L浓度作用下，APC表达增加最多。

The results showed that though testosterone or 17-β-estradiol did not affect the expression of these AM in unstimulated HUVEC, both of them transiently increased the expression of E-selectin and VCAM-1 in TNF-α-stimulated HUVEC.

近几年研究发现，粥样硬化与炎症及免疫反应有关，TNF-α等细胞因子可以通过刺激血管内皮细胞粘附分子的表达及血管平滑肌细胞的增殖等而在粥样硬化的形成中起重要作用。

Profiles of β-actin expression in different recovery time after heat shock were expressed similarly and not significantly different from that of the unstimulated control, suggesting an ubiguitous expression pattern.

表明β-actin是研究受外界环境胁迫作用下昆虫体内不同基因表达水平的可靠内参基因。

In fetal human kidneys, high level of FPC expression is present in ureteric bud and its expression continues throughout the process of renal tubuobranching.

在人类，FPC在胎肾即开始表达于输尿管芽，并持续表达于输尿管芽分支演变为集合管的整个过程。

To determine the effects of ovarian steroid hormones and some cytokines on the expression of RANTES in cultured cells METHODS AND RESULTS The study included the following two parts 1 The Protein Production of RANTES in Peritoneal Fluids and the Expression of RANTES mRNA in Eutopic and Ectopic Endometrium of Patients with Endometriosis Twenty-eight patients with endometriosis were selected as study group and twenty patients without endometriosis and maglinant disease were selected as control group . In our study, 10 samples of peritoneal fluid in Em and 10 samples of in En were taken, 13 specimens of ovarian endometrioma , 3 red peritoneal endometriotic lesions , 2 uterosacral ligament nodules , 17 matched eutopic endometrium and 12 specimens of endometrium in En were biopsied.

研究目的 1 从蛋白水平检测RANTES在子宫内膜异位症患者腹水中的表达，从核酸水平比较RANTES在内异症患者的异位病灶和在位内膜与非内异症患者子宫内膜之间表达的差异，探讨RANTES在内异症患者盆腔炎性反应中的作用；2 体外培养在位和异位的子宫内膜细胞，研究细胞因子TNFα、INFγ、雌孕激素以及PPARγ激动剂15-d-PGJ〓对RANTES表达的影响，探讨这些因素在内异症发病机理及内异症患者盆腔炎性反应中的作用。

Among them group A dendritic cell surface processes were less and cytoplasm vacuolated obviously. 2. Compared the expression of cell surface molecules of 5d, 7d ,10d,14d, we found the CD1a, CD83, CD86, HLA-DR and CD80 expression was significantly increased P.

口腔癌患者外周血单个核细胞诱导培养第5d、第7d、第10d、第14d时，细胞表面DC分子的表达情况：第7d时的DC与第5d相比，CD1a、CD83、CD86、CD80和HLA-DR表达率明显上升P 0。

ResultsThere was no expression of Caspase-3 in group A. The expression of Caspase-3 in group C was decreased compared with that in group B（P＜0.01. In groups B and C, expansion and vacuolization of mitochondria, swollen neurons and apoptotic bodies appeared, which suggested that WBH could induce neuron injury, and the neuron injury in group C was more severe than that in group B.

结果A组大鼠海马区神经元无Caspase-3表达，B、C两组大鼠海马区神经元有Caspase-3表达，B组较C组Caspase-3表达平均灰度值减小。B、C两组大鼠海马神经元超微结构发生改变、细胞器水肿，部分线粒体空化，内质网稀疏，突触前膜、后膜结构不完整，可见自噬体结构；B组大鼠的神经元超微结构损害较C组轻。

Methods: Five patients with parkinsonism or dystonia were assigned to general anesthesia using an modified endotracheal tube.

本实验依照人体实验之相关规定进行，五位患有帕金森氏症或肌张力异常的病人接受神经立体定位手术。

If you can benefit from this book, it is our honour.

如果您能从本书获益，这将是我们的荣幸。