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Copies of the T-DNA, suggesting that a total 65505 sites in rice genome were targeted. 3. Histochemical assay of different organs (leaf, leaf sheath, peduncle, stamens, rachilla, ovules, palea/lemma, root, etc.) revealed various patterns of the reporter gene expression, ranging from expression in only one tissue, in multiple tissues, and simultaneously in majority of the tissues.

组织化学检测不同组织/器官（叶、叶鞘、穗轴、雄蕊、小穗轴、子房、稃、根等）表明，不同Enhancer trap系中报告基因GUS呈不同的表达模式，有的只在一个组织中表达，有的在多个组织中表达，有的在所有组织中均表达。

LP Recco PCR Cloning can efficiently and conveniently construct high quality prokaryotic expression vector of human NY-ESO-1 gene, which provide the expression vector to produce pET-15b-NY-ESO-1 fusion protein.

靶向克隆法可快速、高效地构建人NY-ESO-1基因的原核表达载体，为制备pET-15b-NY-ESO-1融合蛋白奠定了基础。

During the process of nuclear reprogramming, the zygotes of the normal embryos and the donor nuclei of thecloned embryos cease their unique repertoire of gene expression and reset their gene expression to the totipo-tent status, and then the normal and cloned embryos redifferentiate from the totipotent status to variousdifferentiated states for tissue generation or organogenesis.

通过细胞核重编程，首先，受精卵和克隆胚胎的供体核停止其特有的基因表达程序，恢复为全能状态的基因表达程序；然后，受精胚胎和克隆胚胎的细胞再从全能状态重新进入分化状态，最终形成各种组织和器官。

(1)raising of PCNA protein expression after pre-operative radiotherapy of esophageal carcinoma may signify the repopulation of tumor cells.(2) The prognosis differed from different PCNA expression before or after radiation, it may be beneficial to select the best treatment plan and as the evaluating criteria.

(1)食管癌放疗后PCNA蛋白表达升高，可能存在加速再增殖；(2)疗前、疗后PCNA蛋白表达的预测性不同，可指导临床选择最有效治疗方案，可作为评价预后指标。

The tac promoter is now one of the most popular promoters used for gene expression. The Ptac-based expression system was regulated by the lac represser protein which is a product of lad gene.

Ptac启动子是研究基因表达最常用的启动子之一，在Ptac启动子基础上建立的表达系统受lacI基因编码的阻遏蛋白调控。

In 1-7d group, the difference between experimental group and control group was not significant. In 14-21d group, Bcl-2mRNA and P53mRNA were expressed lightly. In 28d group, the expression were comparatively strong. The results indicated that, after exposure to infrasound with some parameters, with the days elapsing, Bcl-2mRNA expression in neurons of mice hippocampus displayed certain regularity. The progressive increase suggested that infrasound could injure hippocampus, and resultantly, injure and repair neurons DNA.

据此，我们认为：当一定参数的次声作用小鼠后，随着作用天数的延长，小鼠海马内神经细胞Bcl-2mRNA表达有一定规律性，总体趋势表达增强，随次声作用次数进一步增多，21d组和28d组有产生适应的倾向，其意义在于次声作用后引起海马损伤，使神经元DNA损伤并产生修复。

C: positive expression of pathologic grade Ⅰ(TN expression also appeared yellowish bands or reticula, but the positive rate is less) SP×400

2.3 MVD与胆管癌临床病理特征的关系经计数，病理分级为Ⅰ级的癌组织中MVD为54.43±6.35，Ⅱ级为61.76±7.42，Ⅲ级为62.89±5.51。

The expression of PKC is increased in most eosinophlis cells of rhinopolypus tissues. Treatment with fluticasone could depress the PKC expression in eosinophlis cell and decrease the infiltration of eosinophlis cells.

蛋白激酶C在鼻息肉组织中呈高表达，糖皮质激素可以有效抑制鼻息肉中蛋白激酶C的表达，减少嗜酸粒细胞的浸润。

However, the value of an entire comma-separated expression is only the result of the rightmost expression.

整个逗号表达式的值是由最右边的表达式决定的。

Observed the expression of Ps and ICAM-1 at 1, 6, 12 hour during normoxia, hypoxia and reoxygenation respectively and the effect of serum safflor injection on the expression of Ps and 1CAM-1 mRNA after reoxygenation.

目的观察含红花血清在缺氧复氧条件下对大鼠肺动脉内皮细胞P-选择素及细胞间黏附分子-1（ICAM-1）基因表达的影响，探讨肺栓塞溶栓后缺血再灌注损伤的机制。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。