查询词典 expression

The expression of MMP-3 mRNA of RA was higher than that of controls,and the expression of MMP-3 mRNA of RA in active stage was higher than that of RA in unactive stage, and there were no difference in different genotypes.

RA患者外周血单核细胞MMP-3的mRNA表达水平高于健康对照人群，有显著性差异，活动期RA患者外周血单核细胞MMP-3的mRNA表达水平高于非活动期的患者，有显著性差异。

Comedy expression shows a combination of happiness, wisdom and thought, and enriches and turns present arts expression.6. As value identity sign in temporal society, sublime in China's teleplay is endowed uncopied modern meaning by consumed background.

喜剧的表现方法呈现了乐感与机智、反思相结合的超越方式，并以喜剧范式中的闯入式结构和肯定性的喜剧人物的塑造，丰富和扭转了崇高既有的艺术表现方式。6、中国电视剧中崇高，作为世俗社会的价值认同符号，消费语境也赋予了崇高不可复制的当代意义：首先，在商品社会中，崇高是因为缺席，成为了消费的内容，并以仪式化的方式构成了一种具有召唤能指的文化符号。

Terminal oxidative inhibitors, such as KCN, Salicylhydroxamic acid , antimycin A and NaN〓, can cut off electron transport chains of cytochrome pathway and alternative pathway, marked decreased BADH protein levels. Oxidative phosphorylation uncoupler 2, 4-dinitrophenol had a similar negative effect on BADH gene expression, and exogenous ATP and ADP increased the BADH levels. These results, in conjunction with the terminal oxidation results, indicated that ATP could be a critical component in BADH gene expression.

2.2 末端氧化抑制剂KCN、SHAM、抗霉素A和NaN〓能打断呼吸链，阻断电子传递，使BADH蛋白的表达量明显降低，说明末端氧化同样参与BADH基因表达的调节：末端氧化影响基因表达的实质就是ATP的影响，线粒体磷酸化解偶联剂DNP明显降低BADH蛋白的含量，而外源的ATP和ADP对BADH基因表达的正效应作用最大。

In this study, we analyze the TATI mRNA expression in HCC and the effect of continuously TATI gene expression in tumor cell: Using the RT-PCR detection, the overexpression of TATI mRNA was found in 154 (69%) out of 224 primary unifocal HCCs, 3 out of 6 hepatoblastomas, 2 out of 7 cholangiocellular carcinomas, 5 out of 6 metastatic colorectal carcinomas. For clinicopathological correlation, the 224 patients with unifocal primary HCC were selected for analysis.

B 在临床病理相关(clinicopathological correlation)分析中，我们选取224个原发性肝细胞癌，发现TATI mRNA过度表现明显好发於女性患者(84% versus 64%，P值=0.0047)，其与血清AFP升高(83% versus 54%，P值=0.00001)、TATI mRNA过度表现较常见於超过5公分的肿瘤(78% versus 60%，P值=0.011)、有早期复发(79% versus 59%，P值=0.0012)及有早期肿瘤转移现象( 83% versus 63%，P值=0.032)的病例呈正相关。

To get more information of the function of human Era on cell cycle, the complete control inducible mammalian expression system was used to express human Era mutant and analyzed cell cycle of uninduced (add 1% ethanol) and induced (10μM Pon A) cell lines by flowcytometer, the primary results demonstrated human Era mutant could inhibit apoptosis induced by ethanol, and Era S36N may stimulate the expression of Bcl-2 as detected by Western Blot.

在构建稳定高表达蜕皮激素受体基因的细胞株3V3的基础上，转染突变型人Era的表达载体进行压力筛选，对所得到的单克隆细胞株进行诱导与未诱导条件下细胞周期的分析，结果表明未诱导加酒精的对照细胞可以引起细胞凋亡，而Era诱导表达后可以显著抑制凋亡细胞的数量，Western表明Era S36N可以诱导细胞Bcl-2表达，提示人Era可能与细胞凋亡相关。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Here were expression of TGFαand EGFR mRNA in unmatured oocyte, matured oocyte, embryo and the expression of TGFαand EGFR in matured oocytes were comparatively more abundant than that in unmatured oocytes.

GFαmRNA的量在8细胞期下降到最低水平，并随着胚胎基因组的激活胚胎开始转录TGFα，使TGFαmRNA表达总量保持在一定水平，持续到桑椹胚期；EGFR mRNA的量在胚胎发育到桑椹胚期下降到最低，而囊胚期胚胎中的整体表达量又上升。

RESULTS: APC promoter 1A was methylated in NCI-H460 cells, and unmethylated in NCI-H446 and SPC-A1 cells. Hypermethylation was detected in all 5 CpG islands (687, 707, 714, 719, 726) of APC promoter 1A in NCI-H460 cells. The expression of APC in NCI-H460 cells was decreased by 26.04% of that in NCI-H446 cells and by 32.36% of that in SPCA1 cells. After treatment of 1, 5, 10, 15μmol/L 5-aza-dC, the expression of APC promoter 1A in NCI-H460 cells was enhanced by 4.59, 5.78, 9.58, 5.98 folds, respectively.

结果：SPC-A1和NCI-H446细胞APC甲基化阴性，NCI-H460细胞APC甲基化阳性；甲基化芯片检测Ncl-H460细胞在APC启动子1A 5个CpG位点均存在甲基化(687、707、714、719、726)，SPC-A1和NCI-H446甲基化阴性，荧光定量结果NCI-H460的APC转录较SPC-A1和NCI-H446有明显的下降，仅为二者平均的30.04%；经5-aza-dC脱甲基化作用后，NCI-H460细胞的APC表达增加了约5~10倍，其中10μmol/L浓度作用下，APC表达增加最多。

The results showed that though testosterone or 17-β-estradiol did not affect the expression of these AM in unstimulated HUVEC, both of them transiently increased the expression of E-selectin and VCAM-1 in TNF-α-stimulated HUVEC.

近几年研究发现，粥样硬化与炎症及免疫反应有关，TNF-α等细胞因子可以通过刺激血管内皮细胞粘附分子的表达及血管平滑肌细胞的增殖等而在粥样硬化的形成中起重要作用。

Profiles of β-actin expression in different recovery time after heat shock were expressed similarly and not significantly different from that of the unstimulated control, suggesting an ubiguitous expression pattern.

表明β-actin是研究受外界环境胁迫作用下昆虫体内不同基因表达水平的可靠内参基因。

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。