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Finally, Prdm16-deficient brown fat displays an abnormal morphology, reduced thermogenic gene expression and elevated expression of muscle-specific genes.

PRDM16（一种锌指蛋白，已知能够刺激棕色脂肪选择性基因表达）可强有力地调控肌肉与棕色脂肪之间的双向细胞命运转换。

To investigate the expression of T lymphoma invasion and metastasis inducing factor 1 (Tiam 1) in tissues of thyroid carcinoma, and to analyze the relation between its expression and invasion and metastasis of thyroid carcinoma.

王化修，甲状腺癌中Tiam 1的表达及临床意义目的探讨T淋巴瘤侵袭转移诱导因子1的表达与甲状腺癌侵袭转移的关系。方法采用免疫组织化学S-P法检测Tiam1在89例甲状腺癌及其淋巴结转移癌的石蜡组织标本中的表达。

Objective To investigate the expression of T lymphoma invasion and metastasis inducing factor 1 (Tiam 1) in tissues of thyroid carcinoma and analyze correlation of the expression with invasion and metastasis of thyroid carcinoma.

目的 探讨T淋巴瘤侵袭转移诱导因子1（T lymphoma invasion and metastasis inducing factor 1，Tiam 1）表达与甲状腺癌侵袭转移的关系。

Objective: To evaluate the expression of Topo-Ⅱ in hepatocellular carcinomaan and to explore the relationship between the expression of Topo-Ⅱ and biological behavior of HCC.

目的：探讨在原发性肝细胞癌中Topo-Ⅱ的表达以及与原发性肝细胞癌生物学特征的相互关系。

Kangdai Ⅰ has protective function to the damaged neurons and astrocytes: Main results:(1) It has direct protective function to the damaged neurons. It can increase the activity and survival rate, decrease the mortality and the transudation rate of LDH in cultured medium and the strong positive cell count of NOS expression of injured neurons.(2) It has also directly protective function to the damaged astrocytes. It can increase the activity and survival rate and protein content in conditioned medium.(3) It can strengthen the ability of BDNF, GDNF, bFGF, HSP and IL-6expression in damaged astrocytes.(4) It can also strengthen obviously the expressions of NSE, bFGF-receptor and bc1-2, lower the expression of bax and caspase-3.(5) It can indirectly protect and restore the damaged neurons by astrocytes. Because the effect of ACMK (ACM interfered by Kangdai Ⅰ) is stronger than ACM+K (ACM associated with Kangdai Ⅰ).

抗呆Ⅰ号对受损的神经元和星形胶质细胞均具有保护作用：主要表现为：(1)对受损神经元具有直接的保护作用，可提高受损神经元的活性和存活率，降低细胞培养液LDH的漏出率、细胞死亡率和NOS染色强阳性细胞的表达量；(2)对受损的星形胶质细胞也有直接的保护作用，可提高其活性、存活率以及培养液蛋白质的含量；(3)能增强受损星形胶质细胞分泌BDNF、GDNF、bFGF、HSP和IL-6的能力；(4)可明显增强受损神经元对NSE、bFGF的受体和bc1-2的表达，降低受损神经元对bax和caspase-3的表达；(5)抗呆Ⅰ号可通过星形胶质细胞间接地保护和修复受损的神经元，因为在多数实验组中经抗呆Ⅰ号作用的ACM的作用远大于ACM与抗呆Ⅰ号联合应用的作用，统计学上具有显著性差异。

Analysis of these mutants revealed that the expression level of the neutral trehalase gene in RNAi mutant (Ma688 strain) was reduced to 42% of which found in wild-type strain and the over-expression mutant (Ma113 strain) showed approximatlely 2-fold higher levels of neutral trehalase mRNA than that found in wild-type control. Then the transformants Ma688 and Ma113 were chosen for closer examination, including trehalose content, storage longevity , thermotol

利用Northern blot 分析这几个突变菌株中性海藻糖酶基因mRNA 的含量，选择中性海藻糖酶基因超表达2 倍和中性海藻糖酶基因被抑制到42%的两个转化菌：Ma113 和Ma688，将其与出发菌株进行分生孢子内海藻糖含量的测定，孢子储藏

These data demonstrated that tributyrin can efficiently inhibit gastric cancer cell growth by inhibiting DNA synthesis and inducing apoptosis. The tributyrin-induced apoptosis is associated with down-regulation of Bcl-2 expression and up-regulation of Bax expression.

这些结果首次显示tributyrin能有效地通过抑制DNA合成和诱导细胞凋亡达到抑制胃癌细胞生长的作用，细胞凋亡与Bcl-2表达量下调和Bax表达量上升有关。

The expression of p-p38 and Rho GDIαwas upregulated in LCA by either low shear stress or hypertension. However, the highest expression of p-p38 and Rho GDIαwas observed in LCA of low shear stress accompanied by hypertension.(4) Effect of low shear stress on both decreasing apoptosis and increasing proliferation of VSMCs in LCA was reversed by tributyrin, a HDAC inhibitor.

当高血压伴有低切应力时，两者的协同作用诱导p-Akt的表达水平进一步增加；(3)高血压和低切应力都可以诱导颈总动脉p38和Rho GDIα表达增加；当高血压伴有低切应力时，两者的协同作用诱导颈总动脉p38和Rho GDIα表达进一步增加；(4)HDAC抑制剂Tributyrin可以通过抑制VSMCs增殖，促进VSMCs凋亡，在干预低切应力诱导的血管重建中发挥作用。

Systematic aerobic exercise upregulates expression of enzyme genes concerning tricarboxylic acid cycle, and downregulates expression of genes concerning muscle protein synthesis and sphingolipid. It is suggested that aerobic exercise is good to protect human nerves' integrity, exerts positive action on anti-aging and accelerate the aerobic metabolism of lipid materials in vivo.

有氧运动可使三羧酸循环相关酶基因表达上调，肌肉蛋白合成相关基因和神经鞘脂类相关基因表达下调，提示有氧运动有助于保护神经细胞的完整性，对抗衰老有积极作用，同时可加速体内脂类物质有氧代谢。

In the present paper, the Sox gene expression analysis of different tissues from the Trionyx sinensis was studied by using RTPCR, and Sox gene fragments of expression from the testicle, brain and spleen were cloned using RTPCR products.

本文采用RT—PCR技术，研究了中华鳖不同组织Sox基因的表达，并通过PCR直接克隆法，分析了来自睾丸、脑和脾组织中的Sox基因序列。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。