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After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Reverse-transcription PCR assays were used to detect the mRNA expression level of related regulatory genes such as p15, p16, p21, p27, p57, surviving, cyclin B, cdc2 and chk1, and Western blot assay to detect the level of protein expression and phosphated change such as survivin, cdc2 and chk1. Results:(1) K562 cell arrested on G2/M phase were obviously increased after co-cultured with 2 to 10μmol/L Arsenic trioxide for 24 hours. The ratios of control group, 2μmol/L, 5μmol/L and 10μmol/L groups were 22.6±3.4%, 27.2±2.3%, 43.8±4.5% and 36.7±4.1%, respectively.

体外培养K562细胞，以不同浓度AS_2O_3作用不同时间后采用PI染色、流式细胞仪检测药物作用后细胞的周期分布改变；逆转录酶多聚酶链扩增方法检测细胞周期相关调节基因(p15、p16、p21、p27、p57、survivin、cyclinB、cdc2、chk1)的mRNA表达变化；Western blot方法检测细胞周期相关调节蛋白表达及磷酸化改变(survivin、cdc2、cdc2-p、chk1)。

Moreoer, c-Fos and phospho–c-Jun protein expression was inhibited by PPARα agonists, indicating that PPARα ligands suppress OPN expression through negatie cross talk with AP-1–dependent transactiation of the OPN promoter.

另外，c-Fos及磷酸化c-Jun蛋白表达可被PPARα激动剂抑制，说明PPARα配体通过OPN启动子AP-1依赖型转录激活的负交互作用而抑制OPN表达。

Methods Expression of the survivin mRNA was evaluated by reverse transcriptase polymerase chain reaction in 76 NSCLC tumor samples,20 benign phymatoid lesion and 21 adjacent normal lung tissue samples Immunohistochemical assay was to detect the expression of P53,c myc,k ras proteins.

研究细胞凋亡的调节机制对于弄清肿瘤包括肺癌的发病机制及新的防治方法具有重要意义。survivin是最近发现的一种凋亡抑制蛋白，在已检测的几种肿瘤组织中表达上调[1 3] ，在肺癌中的表达目前报道甚少，其与

Objective: To optimize phytase expression condition of the recombinant Pichia pastoris and determin expression condition.

目的 优化产植酸酶的毕赤酵母基因工程菌的表达条件，确定最佳的诱导表达条件。

The positive expression rates of VEGF: Pien Tze Huang Group,Lentiviruse mediated-KISS-1 Group and Combined therapy Group, the positive expression rates of VEGF was lower than the other two groups.

VEGF阳性表达率：片仔癀组、KISS-1组和联合治疗组，原发灶肿瘤组织VEGF阳性表达率低于空白组和病毒液组；片仔癀组、KISS-1组、联合治疗组之间相比，无显著性差异。

The epidermis dominant the changes. Mesenchymal is subordination.β-catenin expression begin from placode epidermis stage and go up increasingly as well as peak value maintain to hair follicle. The range of mesenchymal change is less. SHH expression transform is dramatically to stimulate the placode-germ-peg-hair follicle.

结果表明，在睫毛毛囊的形态发生中表皮－间充质相互作用以表皮为主导间充质随着表皮而变化，β-catenin从基板表皮开始表达，逐渐增强，从毛芽至形成毛囊维持高水平，间充质变化幅度较小；Shh则在睫毛形态发生中变化剧烈，刺激基板－毛芽－毛钉－毛囊。

RESULTS: VIP widespread expression existed on photoreceptor-outer, inner nuclear layer, inner plexiform layer of retinal and choroids; weak expression existed on ganglion cell layer and nerve fiber layer.

结果： VIP的表达主要位于视网膜光感受器外节、内核层、内丛状层和脉络膜，在神经节细胞层和神经纤维层有较弱表达。

The recombinant Hansenula polymorpha strains for secretory expression of HBsAg were screened and cultured in YPD, BMMY and MM media respectively. The expression levels of HBsAg were determined by ELISA, and the virus-like particles were observed by transmission electron microscopy.

将其电转化多型汉逊酵母尿嘧啶缺陷型宿主菌ATCC34438(Ura3-)，筛选分泌表达HBsAg的汉逊酵母工程菌株，并比较在YPD、BMMY、MM3种培养基中分泌表达HBsAg的水平，ELISA检测培养上清液中HBsAg的表达量，透射电镜观察类病毒颗粒。

In order to understand the function of this gene within short time, we also constructed yeast (Schizosaccharomyces pombe vector to analyze the gene function, but the result investigated that over expression of this gene could not increase the length of yeast cell. It suggested that expression of the gene is not a direct reason in cell elongation.

为了在短时间内初步研究该基因的功能，构建了酵母表达载体，利用裂殖酵母表达系统对该基因的功能进行活体分析，没有发现该基因对单核的酵母细胞的伸长有明显影响。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。