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The gene for pyruvate kinase,which is involved in glycolysis and catalyze the biochemical reaction from phosphoenolpyruvate to pyruvate,was screened,cloned and sequenced from non photosynthetic Oomycetes,Achlya bisexualis cDNA library.

从真菌藻状菌纲Achlyabisexualis的cDNA文库中克隆了糖酵解途径的最后一种酶—丙酮酸激酶的基因，获得这个基因的完整阅读框架，并且对这个基因的全序列进行了测定。

The result showed 41 clones distribute in 26 OTUs, which exist in Acidimicrobidae and 7 suborders of Actinobacteridae respectively, especially the dominant community of Yutian pit was streptosporangineae instead of streptomycete, which accounting for 42.3% of sequenced clones.

结果表明， 41个克隆序列属于26个OTUs，分别分布于放线菌门放线菌亚纲的7个亚目和酸微菌亚纲，其中链孢囊菌亚目(Streptosporangi- neae)中放线菌组成丰富，占到了全部挑选克隆的42.3%，是于田盐池放线菌群落中的优势菌，而链霉菌不是高盐环境放线菌的优势菌群。

The results showed that the 16S rDNA copies of Bacteroides spp. decreased in rotavirus-infected children by 2 orders of magnitudes. 665 clones in Group H and 284 clones in Group R were sequenced and 34 OTUs were obtained according to 98% sequence homology. Partial least squares discriminant analysis of the clone library profiling data revealed significant structural shifts of gut microbiota.

利用偏最小二乘判别法(Partial least squares discriminant analysis，PLS-DA)对这34个OTU进行分析，结果表明轮状病毒感染导致肠道内拟杆菌组成结构发生了变化，通过拟杆菌的组成可以将健康个体与轮状病毒感染个体分开，而对于区分两组个体起关键作用的OTU分别与Bacteroides vulgatus ，Bacteroides stercoris和Bacteroides fragilis有很高的同源性。

The K-ras gene of Patu 8988 cell was amplified by RT-PCR and the amplification products were directly sequenced.

逆转录－聚合酶链反应扩增人胰腺癌细胞株Patu 8988 K-ras基因，扩增产物直接进行基因序列测定。

Another tropical season legume species, Phaseolus vulgaris ~19 MY diverged from soybean, was chosen and the corresponding orthologous region was defined and sequenced.

另一热带季节豆科植物物种，菜豆 19日，我偏离了大豆，选择和相应的orthologous区域的界定和测序。

They were sequenced and compared with other isolates from different areas of the world and constructed the phylogenetic tree.

用PCR的方法扩增福建 5株样品的Env及LTR区基因，测序后与来自世界各地的代表株进行比较、分析，构建进化树。

Bacterial diversity of sediment in shrimp pond was analyzed by using PCR-DGGE technique, the Shannon-wiener index is 3.4971, the Richness is 41 and the Evenness is 0.94171; 17 dominant bands were excised from DGGE profile of surface sediment bacteria in shrimp pond and the V3 region of 16S rRNA gene was cloned and sequenced followed by phylogenetical analysis.

对虾池表层沉积物细菌多样性进行分析，得出该沉积环境细菌多样性指数为3.4971，丰度为41，均匀度指数为0.94171；对DGGE图谱上的特征条带进行切割回收和克隆测序后得到了优势菌的信息。

HEV-RNA was detected by nested PCR. And positive PCR products were sequenced and analyzed phylogenetically with neighbour-joining method.

对深圳市采集的80份猪粪便标本和6份戊型肝炎病人血清，提取病毒RNA，然后进行PCR检测，阳性时对扩增片段进行基因序列测定，并进行基因型分析。

For better understanding of the phylogenic diversity and evolution of FSH p molecule, we have isolated and sequenced the genomic sequence encoding Chinese swamp buffalo FSH p precursor molecule by PCR.

为了更好地了解FSHβ基因的进化情况，我们利用PCR方法对中国沼泽型水牛的该基因的基因组序列进行了克隆测序。

Taxonomic Study on the Butterfly Subfamily Coliadinae (Lepidoptera: Pieridae) from China;2. Mitochondrial gene fragments of COⅠ and Cyt b of 24 species in the subfamily Pierinae and Coliadinaewere amplified and sequenced.

测定了粉蝶科的粉蝶亚科和黄粉蝶亚科14属共24种线粒体COⅠ和Cyt b基因部分序列，并从GenBank中下载了2种粉蝶的同源序列，以眼蝶科的2个物种为外类群，运用NJ法、贝叶斯法分别重建了分子系统树，探讨了它们的系统发生关系。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。