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In this study, AFLP markers for YrSp gene were identified and SCAR markers were developed using the near-isogenic line YrSp/6 x Avocet S and its susceptible parent Avocet S as materials with the YrSp gene donor parent Spaldings Prolific as control. The main results were given as follows:1. A total of 225 primer combinations were used in AFLP analysis of the materials YrSp/6×Avocet S, Avocet S and Spaldings Prolific.

本研究采用AFLP分析方法,以小麦抗条锈病基因YrSp的供体亲本Spaldings Prolific、近等基因系YrSp/6 x Avocet S、轮回亲本Avocet S及由Avocet S和YrSp/6 x Avocet S杂交制备的F_2代分离群体为材料,开展了小麦抗条锈病基因YrSp的分子标记研究,取得了如下主要结果: 1。

The maps are not,however,desirable and entire because all of the markers on the maps come from the no recurrent parent.The consequence is that number of the markers used for map in backcross population is never more than half in intercross population.

但是,用回交群体作图所得的图谱是不完整的,因为图谱上所有的标记都是非轮回亲本提供的,因此标记数不会超过杂交F2群体的一半。

The absolute positions of rail track can be encoded by the biconditional markers. The train positioning information can be extracted by decoding the exclusive absolute position code. In allusion to the characteristic of the closed-loop track railway, the biconditional markers are arranged according to m sequence.

在轨道线路沿线每个待定位置上设置一个0或1的二值标记,对待定的绝对位置进行编码,通过检测唯一的绝对位置编码值来实现列车的定位。

DNA samples were harvested from 11 Boer billy goat and amplified by 10 selected arbitrary primers,and 113 markers including 31 polymorphic markers were observed.

用 10个随机引物对 11个波尔山羊精液样本DNA进行随机扩增,得到 113个标记,其中多态标记 31个。

In each case, only one of the two possible expected ratios was accepted in all markers, furthermore, no significant deviation from Mendelian inheritance (two-tailed binomial test, P≥0.053). To investigate the polymorphism of AFLP markers, and the genetic diversity in populations of H. discus hannai, eight abalone populations were analyzed by using a total of six AFLP primer combinations. The mean percentages of polymorphic loci of the eight populations were 48.65%, 50.50%, 51.17%, 50.51%, 58.90%, 60.07%, 68.92% and 54.05%, respectively.

皱纹盘鲍群体多样性研究中,选取的6对AFLP引物组合在8个皱纹盘鲍群体内均能扩增出清晰、可重复的扩增产物,扩增带型差异明显。8个群体的多态位点比率分别为:大连养殖群体48.65%、烟台养殖群体50.50%、青岛胶南养殖群体51.17%、青岛崂山养殖群体50.51%、山东长岛野生群体58.90%、山东俚岛野生群体60.07%、日本岩手野生群体68.92%、大连野生群体54.05%;预期杂和度分别为0.2291、0.2438、0.2442、0.2374、0.2626、0.2723、0.3137和0.2802。

In order to find the reason of population decline and assist conservation, the genetic diversity of great bustard was analyzed by microsatellite DNA and mtDNA control region markers. In study using microsatellite DNA markers, Three microsatellites from great bustard O.

为了解东方亚种濒危的现状并为制定科学、有效的保护措施提供科学依据,本研究利用核微卫星DNA和mtDNA控制区序列,从父系和母系两方面对大鸨东方亚种的遗传多样性进行了研究。

The result is stored in the temporary storage unit and is output to the screen by the subprogram "Character generator output". The subprogram "Marker" holds the markers fixed in position in the event of output exceeding their allowable limits, and also handles clearing of the markers

结果被在临时存储单位中储藏和被子程序"个性发电机从输出到"向屏幕使出。,打记号的人把子程序"打记号的人"着力点固定在如果发生在上超过他们的容许极限和也柄的从输出到位置上打记号的人的清除

Typical bands were used to analyze relation with ecotypes and characters of fruits, indicating that there was no relation between typical bands and ecotypes, but some extent relation between bands and characters of fruits existed. If all cultivars were divided into freestone and clingstone, melting and nonmelting, both freestone (ratio shared total survey, 41/56=73.2%) and clingstone (ratio shared total survey, 33/79=41.8%) appeared in the loci of S_(167)-1050 bp linked marker raised by Warburton et al. It was apparently not strong linkage between the marker and the character, and exchange rate of genes was high. If combinations of S_(167)-850bp and S_(167)-1050bp or S_(167)-850bp and S_(167)-1400bp were used to distinguish characters of fruits, 6 freestone accessions without clingstone ones and 6 melting accessions without nonmelting ones displayed in the first combination of markers, 20 clingstone accessions and 2 semi-freestone ones without freestone ones appeared in the second combination of markers.

通过典型带对不同生态型、果实性状为类别进行分析,结果未发现有与生态型相吻合的特征带出现,而将供试所有品种按离核、粘核和溶质、不溶质划分归类,供试品种在Warburton等得到S_(167)-1050 bp处粘离核分子标记,出现既有离核(占供试离核41/56=73.2%),又有粘核(占供试粘核33/79=41.8%),可见此连锁标记连锁性不强,发生交换的机率较高,而S_(167)-850bp带和S_(167)-1050bp带同时出现时,该组包含6个离核,无粘核品种;6个溶质,而无不溶质。S_(167)-850bp和S_(167)-1400bp同时出现时,包含20个粘核品种和2个半离核品种。

Results indicated that,dominant SSR markers or codominant markers with significant difference on length can be separated by optimized SSR amplification system and high concentration agarose gel effectively.

试验结果表明,优化的SSR扩增体系结合高浓度琼脂糖凝胶能够对显性或差异显著的共显性标记进行高效分离。

Markers Xgwm257 and Xgwm319 were resistant codominant, while marker Xgwm296 was resistant dominant. All three markers related to resistance are Ae.

由于该基因不同于定位在小麦2B染色体上的已知抗白粉病基因Pm6和Pm26,应该是一个新的抗病基因,暂定名为PmPS5B,建议定名为Pm32。

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Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严,以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

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Therefore, positively advances the interest rate marketability reform is one of current our country finance reform important tasks.

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