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A highly resistant primitive cultivated species Solanum phureja was employed to generate a mapping population to perform the bulked segregant analysis for screening of AFLP markers linked with the resistance. Another population which had genetic similarity to the mapping population was used for testing of the markers achieved.

以马铃薯青枯病高抗性的原始栽培种Solanum phureja获得的二倍体群体为作图群体进行AFLP标记的初步筛选,另选一个与作图群体有较大亲缘关系和相近遗传背景的二倍体群体对所获标记进行验证。

In this research , by using of genomic in situ hybridization , random amplified polymorphism DNA technology and three specific SCAR molecular markers, the author analyzed wheat hybrid generations carrying the desired genes that were resistant to take-all, BYDV and/or powdery mildew, and developed some alien chromosome addition and translocation lines that were important in breeding practice, and some specific molecular markers that could be applied in marker-assisted selection.

本研究运用GISH、RAPD和SCAR分析方法,对抗全蚀病、黄矮病和兼抗黄矮病白粉病的小麦杂交后代材料进行分析,获得了一些对育种实践有重要意义的易位系、异附加系材料和几个可用于辅助育种的特异性分子标记。

On an unlined field, the boundary is defined as an imaginary line between two field markers with the thickness of said markers.

没线的场地使用两个角标,线的宽度相当于角标的宽度。

Dealing with discourse markers as indicators of metapragmatic awareness, Verschueren's account emphasizes the function of discourse markers in signaling the utterer's mental state in making linguistic choices.

Verschueren认为话语标记语是标示说话人元语用意识的一种语言手段,具有反映说话者选择语言时的元语用意识的作用。

Genetic cluster analysis is similar based on the results of morphological and DNA markers, the five populations could be divided into three groups. But there are different groups sort of individual locations, reflecting the similarities and difference about the analysis of the relationship between population with the two kinds of markers, It could be concluded that there was coupling and relevance to some extent in evaluating genetic diversity with the same experiment sampling by morphological and SSR marker variation. However, there was also differences. In research, the two can complement each other.8. Identification of Genetic Relationship of Amygdalus plants by SSRThe genetic relationship among 55 Amygdalus plants collections, belonging to 6 species and collected from both homeland and abroad were identified via SSR molecular marker technique.

新疆野扁桃遗传多样性不同研究分析方法结果的比较与评价表型与DNA二种水平的遗传标记方法揭示的遗传多样性水平有差异,表型性状比DNA分子标记具有更大的变异性;其受到环境的影响较大,也即对环境更敏感。2种水平的遗传标记聚类结果将野扁桃5个不同分布群体均可分为3个大类群,但是出现有个别不同的群体排序位置,反映了2种标记在分析种群间关系上的异同性,从本研究结果来看,说明表型与DNA标记有一定的耦合性和相关性,但也存在差异,在研究上可以互为补充。8。

In recent years, application of molecular markers such as AFLP, RAPD and SSR markers have speeded up the construction of Cucurbita genetic map.

近年来,AFLP、RAPD和SSR等分子标记技术的运用,加速了瓜类作物分子遗传图谱的构建。

To fine mapping the resistance gene, STS markers weredesigned using some RFLP probes and ESTs mapped on the long arm of chromsome 5ALto densify markers on the chromosome region surrounding mlm404, and the STS markermore closely linked to mlm404, MAG1493, was identified.

为了精细定位该基因,我们用5AL的RFLP探针和EST开发的STS标记丰富了mlm404附近的标记密度,发现了与其更为紧密连锁的STS标记MAG1493.mlm404与大麦抗白粉病基因Mlo直系同源基因TmMlo紧密连锁,遗传距离为1.0 cM。

Methods: 76 patients with colorectal cancer were collected in our department. They were divided into two groups, patients with metastases(MCRC,N=51) and patients after eradicative operation(non-metastasis group, NM,N=25).Serum markers of VEGF, OPN, CA19-9 and CEA were detected in all the included patients,and then to analyze the diagnostic value of the serum markers in detection of metastases.

测定76例结直肠癌患者(其中51例为转移性结直肠癌,25例为术后无转移者)血清中的VEGF、OPN和CA19-9及CEA水平,分析这些指标诊断结直肠癌转移的临床意义。

2N gametes are the result of meiotic mutation during micro- and mega-sporogenesis that bear the sporophytic rather than the gametophytic chromosome number. This paper reviewed the genetic markers including the morphologic, cytological, isozymes and DNA markers, which have been employed in the generation, inheritance, heterozygosity and marker-assisted breeding of 2n gametes based on the frequency of large pollen grains, cytological analyses, unexpected occurrence of polyploidy progeny and the associations between parents and progenies.

文章综述了植物2n配子发生及其遗传标记研究现状,论述涉及有关形态学标记、细胞学标记、同工酶标记乃至DNA标记等遗传标记在2n配子研究中的应用,指出通过花粉形态观察、大小孢子母细胞减数分裂行为观察、杂种后代倍性鉴定以及亲子分子标记相关分析等,对2n配子发生、2n配子遗传类型与杂合性以及2n配子在育种实践中的有效性等进行研究。

The average percentage of polymorphic bands are 72.06% for ISSR and 66.67% for RAPD respectively. The genetic diversity of ginkgo revealed by ISSR (the average effective number of alleles is 1.8780, the average Nei"s gene diversity is 0.4652, and the average Shannon"s information index is 0.6574) are all higher than those by RAPD (the average effective number of alleles is 1.7842, the average Nei"s gene diversity is 0.4317, and the average Shannon"s information index is 0.6211), while the standard errors of the parameters estimated by ISSR were lower. Therefore, ISSR molecular markers are more suited than RAPD molecular markers when testing the genetic diversity of ginkgo populations and determining the genetic relationship of among populations or among individuals which are much similar hereditarily.

本文应用RAPD和ISSR两种分子标记技术,选取中国5个可能的野生银杏居群共计75个样品,对其遗传多样性进行了研究,得出以下结论:(1)用筛选出的12个RAPD引物和10个ISSR引物进行PCR扩增,分别扩增出65条和68条重复性高、清晰的条带,多态性位点百分率分别66.67%和72.06%,ISSR揭示的银杏遗传多样性(平均有效等位基因数目为1.8780,平均基因多样度为0.4652,平均信息指数为0.6574)高于RAPD所得到的结果(平均有效等位基因数目为1.7842,平均基因多样度为0.4317,平均信息指数为0.6211),其所估算参数的标准差要低于RAPD所估算出的值,因此,在研究亲缘关系非常近的银杏物种的遗传多样性并试图确定居群间或个体间的遗传关系时,ISSR分子标记技术比RAPD分子标记技术更为合适。

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Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

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