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Compared with the corresponding BEAS-2B cells, the shape of cell clone, the way of cell growth, the shape and quantity of cell and nuclear were not significantly changed in the 1st, 2nd, 5th passage BEAS-2BNNK cells. The shapes of cells and its nuclears of the 10th, 15th, 20th, 25th passage BEAS-2BNNK cells became round gradually, and the way of cell growth became from radio-form into membraniform and more than one nucleolus appeared.

BEAS-2BNNK细胞在第1、2、5代时细胞克隆形态及生长方式、细胞形态、细胞核形态及数目与相应代BEAS-2B无明显差异；第10、15、20、25代时细胞及胞核逐渐变圆，出现多个核仁，细胞由放射状生长逐渐变为膜状生长。

In measurements of a day,generallyspeaking,the photosynthesis of birch in mesophytic habitat is better than that in xerophytichabitat(peak values are 12.8,10.33μmolCO2m-2s-1 respectively);that of sexual birch inmesophytic habitat is better than that of clone birch(peak values are 9.87,6.71μmolCO2m-2s-1respectively);that of young tree is better than that of seedling(peak values are12.37,10.05μmolCO2m-2s-1 respectively).

在一天中的各个时刻，总体说来，中生生境生长的白桦光合作用超过旱生生境生长的白桦光合作用(净光合速率峰值分别为12.8、10.33μmolCO2m-2s-1)；白桦幼树的光合作用超过白桦幼苗(净光合速率峰值分别为12.37、10.05μmolCO2m-2s-1)；中生生境有性白桦的光合作用超过无性白桦的光合作用(净光合速率峰值分别为9.87μmolCO2m-2s-1、6.71μmolCO2m-2s-1)。

Man takes the reins of the most delicate tiny mysteries of microcosmos by means of anatomize gene and clone life.

剖析基因，克隆生命，人类掌握微观世界最微妙的奥秘。

METHODS: NSCs were isolated from neonatal rats by enzyme digestion and mechanical separation. At the fourth passage, cell clone masses received nestin immunocytochemistry. Remaining cells were dispersed by mechanical separation. Monoclone NSCs were incubated by limiting dilution assay, and made into 108 L-1 monoplast suspension in complete medium. NSCs were assigned into 2 groups. NSCs in the control group were incubated in 10% fetal bovine serum.

酶消化和机械分离法相结合体外分离培养新生鼠神经干细胞，传至第4代的细胞克隆团行巢蛋白免疫细胞化学染色观察，剩余细胞团用机械法分散，采用有限稀释法进行单克隆神经干细胞培养，加入完全培养基制成108 L-1的单细胞悬液，分为2组滴入培养板，对照组加入10%FBS，诱导组加入10%FBS+50 μg/L神经生长因子，培养5~7 d。

Objective:The purpose of this paper is to clone novel leukemia associated gene in acute myeloid leukemia.

目的：发现新的白血病相关基因及探讨白血病的发生机制。

A phenylalanine ammonia-lyase gene was clone from Nerium oleander for the first time and it was also the first cloned gene involved in anthocyanin metabolic pathway in N.oleander.

首次从夹竹桃中克隆得到苯丙氨酸解氨酶基因cDNA片段，并命名为NoPAL1，GenBank登录号为AY747677，这也是首次从夹竹桃花青苷代谢途径中克隆得到的基因。

Preliminary location of the nosogenic gene was carried out successfully. It is the foundation of clone of the nosogenic genes and might be widely used for gene diagnosis in the fijture.

成功地对致病基因进行了初步定位，为进一步克隆致病基因，进而实现临床上疾病的基因诊断奠定了基础。

A new picorna-like virus was isolated from dead Evtropis oblique larvae deceased from NPV infection. Electron microscopic observations of purified virions were non-enveloped isometric particles with diameter of 26nm. The virions contain two capsid proteins: 31.5kDa and 28.8kDa, the amount of later is 2.5 times more than that of the former. Analysis of 3′terminal sequence of EoPV clone identified that it can encode RdRp and has eight conserved motifs. Homology analysis shows that it is closely related to Perina nuda picorna-like virus.

从核型多角体病毒感染致死的茶尺蠖幼虫尸体中分离到一株微小RNA病毒透射电镜观察纯化的病毒粒子为无囊膜、无表面特征、直径约26nm的球状颗粒。16%的SDS-PAGE显示它有两个分子量为31.5 kDa和28.8 kDa的衣壳蛋白，后者的含量是前者的2.5倍。3′端克隆序列分析表明EoPV基因组3′有poly尾，编码RNA聚合酶，含有微小RNA病毒RNA聚合酶的八个保守基序，同源性分析表明它与榕透翅毒蛾微小RNA病毒亲缘关系最近。

Ooplasmic transfer increases the fertilizability of the aged oocytes and the early embryonic viability, it has led to the birth of health babies, and become a hotspot in the realm of human assisted reproduction biotechnology and animal clone.

卵胞质转移技术能有效改善老龄卵母细胞的受精能力和早期胚胎的发育能力，在人类已有健康后代出生，它已成为人类辅助生殖生物技术和动物克隆研究的新热点。

Orchidaceae ; DNA molecular marker ; Gene clone ; Genetic transformation ; Flowerdevelopment ; Molecular biology

兰科植物；分子标记；基因克隆；转基因；花的发育；分子生物学

It has to be computed by the host for the actual clock rate.

主控制器可以用来计算实际的时钟速率。

Let's see what you got.

让我看看你的东西