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In the condition of suspending culture, the gametophytic clone has much higher instantaneous growth rate than stilling culture.

在充气悬浮培养条件下克隆的瞬时生长率明显高于静置培养。

60Co-γray was a useful mutagen in induced mutation breeding .After treated with 60Co-γray ,free-living conchocelis of wild Porphyra haitanensis were induced and represented in offsprings. Purple mutant and excellent practical characters strains were selected from a lot of gametophytic blades. Single cell clone and enzymolysis were used to propagate fast in pure strains of induced mutations.

坛紫菜野生丝状体经一定剂量60Co-γ射线辐照后，子代叶状体发生了变异，经大量培养后筛选出紫色突变体（3号Ⅰ）和经济性状优良的个体（7号Ⅰ和7号Ⅱ），通过体细胞酶解和单克隆技术快速获得诱变选育纯系，并对其主要经济性状进行了研究。

The result indicates the length of the clone sequence is 963bp and shares a similarity of 45.24 % with the published Glycine max glycinin subunit G7 (Gy7) gene promoter.but have more similar to quantity and distance of promoter elements, they all contained typical TATA-box, CAAT-box and necessary regulatory motifs of seed-specific expression.

测序结果表明所克隆的片段长为963bp，与预期设计的大小一致，该片段与已发表的11S球蛋白基因启动子序列的相似性为45.2％，但所含的启动子序列元件的数量和距离上很相似，均具有有典型的TATA盒和CAAT盒，以及种子特异表达所必须的调控元件。

A new full-length cDNA clone was isolated from the fiber cDNA library of Gossypium hirsutum L.

利用PCR筛选方法从陆地棉纤维cDNA文库中筛选到一个全长cDNA序列，命名为GhbZIP。

In the research, one kind of guzmania species named ostara was employed to be as material, and the full-length cDNA plasmid library of floral organ was constructed successfully by using SMART technology. The library had 3×10^6 original titer and more than 1 kb insert fragments. After 5'EST sequencing from 2004 positive clone chosen at random and clustering analyse, 1 758 high-quality sequences and 1 365 unigenes including 175 contigs and 1 195 singlets were obtained. These unigenes had 1 283 valid ORFs. COG analysis showed that those proteins coded by EST sequences were divided into 22 classes. Through blast analysis, full length or part cDNA of some genes controlling flower color, development of floral organs, florescence regulation and other breeding value genes were obtained.

本研究以擎天凤梨属品种Ostara为材料，采用SMART技术成功构建了擎天凤梨花器官的质粒型全长cDNA文库，初始文库滴度为3×10^6，插入片段平均长度大于1kb；随机挑取2004个阳性克隆进行5'EST测序，获得高质量序列1758条，经拼接获得1365条单基因簇，其中跨叠群175个和单条序列1195个；经ORF寻找共获得有效ORF1283条；经COG分析EST序列编码的蛋白质被分为22类；经Blast分析后，获得一批花色、花器官发育、花期调控以及其它育种价值基因（包括cDNA全长或片段）。

Objective Chronic myeloid leukemia, with of chromosome, is one of primary clonal malignancies of hemapoietic stem cell. Philadelphia chromosome is the hallmark of malignancies clone in CML.

研究背景与目的：慢性髓系白血病是一种原发于多能造血干细胞的恶性克隆性疾病，第9号与第22号染色体相互易位形成Ph染色体是其肿瘤恶性克隆性标志。

False positive were lowered to an acceptable rate by this protocol, therefore, a big amount of work on fingerprint to get rid of false positive clones can be avoided. When whole BAC clone containing one locus of a multi-loci probe were applied for library screening, no matter homogenic BAC or heterogenic BAC, site-specificity is higher than short DNA fragment probes.

对于无法通过小基因组填补间隙的区段，可以本物种BAC克隆直接进行染色体步移，采用抑制重复顺序杂交法能够有效抑制大量存在于植物基因组中的重复顺序的干扰，把假阳性率降低到可以接受的范围，从而避免了用大量指纹分析剔除假阳性克隆。

Findings in clinical research show that life regenerative substances can activate and culture the healthy organ cells to regenerate and clone, while at the same time, these life regenerative substances can prevent the normal cells from canceration and can induce apoptosis of the cancerous tumor cells. Based on the animal experiments, we have successfully produced the nutritious foods with life regenerative substances according to the national food safety standards. Since Jan 25th 2008, more than 300 cases of cancer patients who had given up the traditional medical treatment accepted our holozoic diet therapy with the life regenerative substances, and remarkable regenerative effects have been obtained. The potential regenerative ability of the organ cells are actived by the life regenerative nutrients, thus the cancer patients continue to struggle against with cancers.

我们研究发现，人体器官的再生物质，在促进正常细胞再生克隆的同时，能阻止细胞被诱导成癌细胞，能使癌细胞凋亡性死亡，从而在进行了动物实验的基础上，将这些再生营养物质制作成符合国家标准的营养食品，于2008年1月25日开始进行了300多例放弃医学治疗的临终癌症病人的全营养食疗养生，获得了再生复原的效果，养生的病人仍能通过再生营养的作用，调动人体的再生潜能，继续与癌症病魔作斗争。

To screen the sensitive and resistant yeast mutant when they treated with drug, the recombine technology of yeast gene should be applied to obtain the clone expressing the homeotic gene.

通过使用药物对特定的酵母基因突变株进行干预，筛选出对药物敏感或抵抗的突变株，然后利用酵母基因重组技术，使突变株再表达同源的基因，从而获得再表达同一基因的克隆。

The sequence of amino acid of clone CR271 was highly homologic (61% identify and 76% similarity) to blight resistance gene Xal of rice to Xanthomonas oryzae pv. Oryzae. All other fragments showed significantly amino acidhomology to the NBS-LRR type resistance gene that have been cloned. The length of JR2 and JR3 fragments were 1321bp and 896bp respectively, which did not showed significantly homology on the level of amino acid and nucleotide acid to the sequences that have been known.

其中CR271与水稻白叶枯病抗病基因Xa1(AB002266)氨基酸同源性有61％相同，76％相似，其它片段与已克隆的NBS-LRR类抗病基因同源片段都具有较高的同源性；所获得的右翼序列JR2DNA片段长为1321bp，JR3DNA片段长为896bp，此两片段在核苷酸水平及氨基酸水平上均未找到有显著同源的序列。

It has to be computed by the host for the actual clock rate.

主控制器可以用来计算实际的时钟速率。

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