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Lucidum. Then we clone the gene, glfks1 and glfks2. Hydropathy analysis predicts that GlFks1 and GlFks2 are integral membrane protein with about 16 and 14 transmembrane helices.In genomic southern blot analysis, glfks1 and glfks2 both are single copy gene.

cerevisiae相关之β-(1，3)-D-葡聚醣合成酶基因fks相似之序列，并将其选殖出来，分别得到glfks1与glfks2，利用hydropathy分析，显示GlFks1与GlFks2分别具有16个与14个transmembrane helix，且推测GlFks1与GlFks2应也是属於嵌入的membrane protein。

Theoretically, its calculation complexity is studied, while it is tested empirically by learning multi-samples generated randomly. The comparative analysis and applications show it has some merits of rapid searching and good clustering effect. B3. An immune network optimization algorithm with information feedback, which can solve special complex function optimization problems with high dimensions which objective functions are formed by summing multi-subobjective ones, is constructed based on the clone selection principle and the immune network regulation of the idiotype immune network.

为了对免疫系统中抗体如何学习抗原特征的整体行为有一定的认识，从单个抗体识别抗原及多个抗体并行识别抗原的特征出发，利用免疫应答的相关原理及免疫细胞的免疫功能设计了适合于批量样本聚类的抗原特征学习算法，理论上分析了其计算复杂度，获得了此算法中的关键参数与其搜索性能的关系；应用上，该算法通过对实际的多样本学习，获得了搜索速度快及聚类效果好的优点，比较分析表明这种方法是可行的和有效的。

Restrictive changes of TCR Vp repertoire and clonal expanded T cells could be found in peripheral blood T cells from patients with T-ALL. However, the clonal expanded T cells' property (clonal expansion leukemic cells or leukemia antigen-specific expansion T cells) should be further characterized. It may play a certain role on detection of minimal residual disease and design of anti-leukemia idiotypic vaccine. The dominant utilization of TCR VjJ repertoire could be found in peripheral blood T cells from patients with B-ALL, meanwhile clonal expansive T cells were existed. It may be a feature of the host immune response for leukemia-associated antigen. The clonal expansive tendency of T cells in V021 and VP23 subfamilies was rather obvious, which may be correlated with certain malignant B-cell clone. The CDR3 sequences of monoclonal expansive T cell in T cell strains were different, which was the base of developing DNA vaccine.

T-ALL患者外周血T细胞的TCR Vβ谱系出现限制性改变，均可检测到克隆性增殖T细胞，尚需进一步鉴定其性质(肿瘤性或抗原特异性增殖)，对于研究微小残留病变检测和设计抗白血病独特型疫苗均有一定的意义。B-ALL病人外周血T细胞的TCR Vβ谱系呈现优势利用的特点，并存在克隆性增殖的T细胞，这可能是机体对白血病相关抗原产生的特异性免疫反应；Vβ21和Vβ23亚家族T细胞发生克隆增殖改变的趋向性比较明显，可能与B-ALL相关抗原刺激有关。T细胞株中检测到的肿瘤克隆其CDR3序列具有高度的特异性，是构建DNA疫苗的基础。

Methods: Clone the genes of ScFv (OC183B2) and PE38 into PET30a and then express the immunotoxin after induced with IPTG.

采用基因工程技术，经过3次亚克隆，将编码单链抗体的基因及编码毒素的基因亚克隆至原核表达载体pET30a上，并在IPTG的诱导下进行表达。

The distribution of PTA1 molecule on NK cell, NK cell lines and NK cell clone was detected by indirected immunofluorescence staining and FCM analysis. The results showed that PTA1 expression rate on activated NK cell in MLC was above 60%.

结果证明这种方法能够稳定获得NK细胞克隆，应用流式细胞仪检测获得的一株NK细胞克隆表型为 CD56CD3－。

We construct pCDNA3.1-TfRexpression plasmid successfully, there are higher expression of TfR aftertransient and stable transfection; we get positive cell clone of containingpCDNA3.1-TfR, establish infarctate foundation to empirical study ofmolecular imaging of MR.

成功构建了pCDNA3.1-TfR表达质粒；质粒转染U251细胞后瞬时与稳定表达的TfR蛋白明显增高；成功筛选出pCDNA3.1-TfR阳性细胞克隆，为分子影像学的实验研究奠定了坚实的基础。

In order to reach this goal,the primary basic research work is to clone,evaluate and analyse the function of the genes that support or impact the development of malaria parasites in the mosquito vecter and the study on the mechanisms of innative immune defence responses against Plasmodium infection.

于上世纪九十年代，WHO提出了基因操纵蚊媒的新策略，而寻找支持或抑制疟原虫在蚊体内发育的相关基因，克隆、鉴定及分析其功能，以及研究按蚊抗疟原虫感染的先天性免疫反应机制是实现这一策略的重要前提。

Results (1)Multiple copies of pMCLacI/Neo plasmid DNA were intactly integrated in the genomic DNAs of the cell clone.(2) One of lac Ⅰ target genes in the integrated plasmid could be transcribed in the NIH3T3 cells while the other could not.

因此，在这些突变婴儿检测系统中，通过一些不表达的中性靶基因所婴儿检测到的突变规律肿瘤是否代表体内一些重要的功能基因的真实情况，目前尚不清楚［1-3］。

Results (1)Multiple copies of pMCLacI/Neo plasmid DNA were intactly integrated in the genomic DNAs of the cell clone.(2) One of lac Ⅰ target genes in the integrated plasmid could be transcribed in the NIH3T3 cells while the other could not.

因此，在这些突变检测系统中，通过一些不表达的中性靶基因所检测到的突变规律是否代表体内一些重要的功能基因的真实情况，目前尚不清楚［1-3］。

Different from the importation characteristic before 90s, strains after 90s were highly isogenous, suggested that the cholera epidemics in Sichuan province after 90s were endemic source. Inaba 1d strains from national wide epidemic in 2001 and 2002 were the same RT. Strains typed as different phagetypes (phagetype 6, phagetype 13 and phagetype 1) might be from a same clone.

除82-007、95-001外，所试菌株均具有致病性；20世纪90年代以四川省发生的霍乱为输入性，90年代后四川省流行的菌株高度同源，具有地方疫源性；2001、2002年在四川省乃至全国引起流行的稻叶1d为同 RT型，为传入性霍乱；噬菌体分型6型、13型菌株和1型菌株可能来自同一克隆系。

It has to be computed by the host for the actual clock rate.

主控制器可以用来计算实际的时钟速率。

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