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Different from the importation characteristic before 90s, strains after 90s were highly isogenous, suggested that the cholera epidemics in Sichuan province after 90s were endemic source. Inaba 1d strains from national wide epidemic in 2001 and 2002 were the same RT. Strains typed as different phagetypes (phagetype 6, phagetype 13 and phagetype 1) might be from a same clone.

除82-007、95-001外，所试菌株均具有致病性；20世纪90年代以前四川省发生的霍乱为输入性，90年代后四川省流行的菌株高度同源，具有地方疫源性；2001、2002年在四川省乃至全国引起大流行的稻叶1d为同一RT型，为传入性霍乱；噬菌体分型6型、13型菌株和1型菌株可能来自同一克隆系。

To clone the calmodulin genes from Karelinia caspica, Kalidium foliadum and Betula halophila in Xinjiang, designed primers were used to amplify 450-bp cDNA fragments with RT-PCR.

提要 采用RT-PCR扩增的方法，从新疆盐生植物花花柴、盐爪爪和盐桦中分别克隆获得了450 bp的cDNA片段。

A strain of breast tumor cell line ZHL2006 was established and biologically identified. The ZHL2006 cells grew fast in vitro and the population doubling time was 43.6h. Electronmicroscopical observation showed that there were microvilli on the surface of the cells, nucleus irregularity, karyoplasmic ratio increased, nucleolus distinctness, et al.. Chromosome number and structure were abnormal. Immunohistochemistry showed that PCK were expressed. The ZHL2006 cell line could form onto clone in soft agar and had oncogenicity in athymic mice.

ZHL2006细胞在体外生长迅速，群体倍增时间为43.6h；倒置相差显微镜及Giemsa染色镜下观察细胞为多边形及梭形；透射电镜观察可见细胞表面有微绒毛，核高度不规则，核浆比例增大，核仁明显等；染色体数目和结构异常；广谱细胞角蛋白染色阳性，说明其自上皮而非来自基质；同时ZHL2006细胞系在软琼脂中可形成克隆，具裸鼠致瘤性。

Objective To clone and sequence variant-specific surface antigen gene from Giardia lamblia isolate SUCH/89/BTMRI/2(C2) derived from human in China.

目的 克隆并测定中国人源蓝氏贾第鞭毛虫滋养体SUCH/89/BTMRI/2(C2)的表面变异抗原基因序列。

Objective To clone and sequence variant-specific surface antigen gene from Giardia lamblia isolate SUCH/89/BTMRI/2（C2） derived from human in China. Methods Total genomic DNA of G.

摘要目的克隆并测定中国人源蓝氏贾第鞭毛虫滋养体SUCH/89/BTMRI/2（C2）的表面变异抗原基因序列。

Avian leukosis virus ; subgroup J ; env gene ; clone ; expression

禽白血病病毒； J亚群； env基因；克隆；表达

Convey albumen immunity with rarefied LTb 8 Zhou Ling BALB/c rat 3 hind, after taking lienal cell and cell of SP2/0 bone myeloma to undertake confluence, undertake filtration with indirect ELISA and finite and attenuant law, obtain 4 individual plant to be able to stabilize the individual plant of cross tumour cell that secretes antibody of Chan Kelong of LTb of bacili of bowel of the Chinese People's Anti-Japanese Military and Political College, antibody of odd clone of this individual plant names for 1F2, 2C2, 3D6, 4H6 individual plant.

用纯化的LTb表达蛋白免疫8周龄BALB/c鼠3次后，取脾细胞和SP2/0骨髓瘤细胞进行融合后，以间接ELISA和有限稀释法进行筛选，获得4株能稳定分泌抗大肠杆菌LTb单克隆抗体的杂交瘤细胞株，该株单克隆抗体命名为1F2，2C2，3D6，4H6株。

We employed an easily observable and manipulatable system in which activated and non-activated T-cells were co-cultured with a stable well-characterized clone of lacZ-expressing murine NSCs.

我们采用了直观且简单易行的系统，将活化和未活化的T细胞与稳定的lacZ表达的鼠类NSCs共培养。

RbcL gene was obtained by polymerase chain reaction and cloned into pDM19-T vector. The positive clone identified by PCR was sequenced. The nucleotide sequence was 1266 bp long, including an ORF of 1031bp, encoding a putative 343 amino acids. The deduced amino acid sequence had a high identity above 94.13% with that of tobacco, spinach, sweet potato, corn, Arabidopsis thaliana, Oryza sativa, Vitis vinifera, Marchantia polymorpha and Citrus paradise.

阳性克隆鉴定后进行测序，序列分析结果表明：该基因片断长为1266 bp，包括1031 bp的编码区序列，编码343个氨基酸；其编码区氨基酸与烟草、菠菜、玉米、甘薯、拟南芥、水稻、葡萄、地钱和葡萄柚等9个物种的同源性94.13%以上，并构建了它们间的亲缘关系树。

Chieftain with the degenerate primers by RT-PCR.The cDNA clone of 3GT encodes a predicted polypeptides of 448 amino acids,and the deduce protein showed 76% highest identity with 3GT of Petunia hybrida and Solanum melongena,and the multiple alignment and phylogenetic analysis demonstrated that it belong to a new member of the corresponding 3GT-family enzymes.

序列分析表明，这个3GT基因编码的多肽为448个氨基酸残基，在氨基酸水平上与茄科的矮牵牛和茄子的一致性最高，均为76%，多重比较和系统发育分析均表明，该基因为3GT家族中的一个新成员。

Panorama Many people are dancing in a large sitting room.

在一个大客厅里，许多人在跳舞。

We fully understand how a microwave oven cooks food, it is first necessary to talk ...

我们充分理解一个微波炉煮的食物，这是首先要谈。。。