查询词典 blot

The lysate of the cells were collected to detect the expression of the two genes by Western blot.

pylori与AGS细胞共同培养1h，用Western blot方法检测细胞内c-Fos和c-Jun基因表达的变化。

The new antigen P3 peptide which harvested from the 430A peptide synthesizer were verified by the mass spectrograph and HPLC, that the ratio of mass/charge of the synthetic peptide was equal to the molecular weight in theory and its purity was above 95%. The Western blot analysis show that the antiserum induced by the new antigen plus hemocyanin could bind with the 22KD and 55KD proteins, which were existed in the testis tissue protein of mouse, rat and human.

一、经抗原分子设计，在430A自动肽合成仪上合成的新抗原P3，经质谱仪分析证明其荷质比与理论一致；纯化后，其纯度大于95％；将新抗原与KLH偶连后免疫小鼠，经Western blot证实其诱导的特异性抗体可识别小鼠、大鼠、人睾丸组织中分子量约为22KD和55KD左右的蛋白质。

The expression of MDK was detected by real-time PCR and Western Blot. Results: The retroviral vector pLXSN-MDK was successfully constructed and infected SGC7901 cells,and the MDK gene expression of SGC7901/pLXSN-MDK was up-regulated.

病毒上清感染SGC7901细胞，G418筛选出阳性克隆，经实时定量PCR及Western Blot检测发现SGC7901细胞中MDK mRNA及蛋白表达水平明显上调。

The expressed product was purified by affinity chromatography and identified by Western blot, then determined for enzyme activity by Micrococcus lysodeikticus turbidimetric method and for biological activity by heat shock method.

表达产物经亲和层析纯化后，进行Western blot鉴定，并用溶壁微球菌比浊法检测酶活性，热激法检测其生物学活性。

Results In the transgenic mice after microinjection, there were 4 Southern-blot positive mice, and the expression of bcl-xL mRNA and protein was detected.

结果 在显微注射所产生的转基因小鼠中，有4只为Southern blot检测阳性，并且在这4只小鼠及其子代中均检测到人bcl-xL的mRNA和过表达的目的蛋白。

IgE reactivity of recombinant Caryota mitis pollen profilin was investigated by western blot.

重组短穗鱼尾葵花粉profilin在大肠杆菌中高效的表达，进一步经Ni2＋亲和层析柱纯化后经Western-blot检测具有良好的免疫学活性。

Expression of peripheral monocyte MMP-9 was measured by RT-PCR and Western blot.

分离大鼠外周血单核细胞，用RT-PCR和Western blot检测MMP-9表达的变化。

Western Blot was utilized to evaluate the morphism on the expression of cyclin D1, Cdk6 and pRb and phosphorylation of pRb.

Western Blot法检测Cyclin D1基因多态性对Cyclin D1、Cdk6和pRb的表达水平以及对pRb磷酸化水平的影响。

The recombinant baculovirus was transfected into sf-9 cells by CellFectin reagent. SDS-PAGE and Western-blot analysis showed that the expressed protein was 37ku in molecular mass and exhibited specific immunological activity. These results provide foundation for development of a subunit vaccine with the expressed σC protein against Muscovy duck reovirus infection.

在脂质体介导下将rBacmid-σC转染sf-9昆虫细胞，获得重组杆状病毒rBV-σC；SDS-PAGE和Western-blot分析表明，在sf-9细胞中表达了分子质量约37ku的σC蛋白，该蛋白能与原核表达的σC蛋白免疫小鼠血清发生特异性免疫反应，这为以表达的σC蛋白为抗原的亚单位疫苗的研制奠定了基础。

In this paper, the complete nucleotide sequence of watermelon mosaic virus Chinese Isolate was determined. On the basis, the HC-Pro protein and CP protein both are the factors for aphid transmission were cloned and expressed in vitro, and their antiserum were prepared. By far western blot, viral receptors were identified in aphids, and their interactions were studied. The molecular mechanism of nonpersistent transmission was further elucidated.

本文完成了我国西瓜花叶病毒的基因组全序列的测定，在此基础上，克隆和体外表达了蚜传因子HC-Pro和CP蛋白，制备了相应抗体，利用Far western blot技术鉴定了其蚜虫体内的受体蛋白，并对三者互作关系进行研究，进一步阐明了非持久性传毒的分子机制。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。