查询词典 blot

MPGES-2 immunostaining remained a strong level from day 1 morning to day 2 evening, slightly increased from days 3 to 4, and dropped to a low level from days 5 and 6. During early pregnancy, mPGES-2 expression pattern shown by Western blot was similar to that of infundibulum by immunohistochemistry.

利用Western blot检测mPGES-2蛋白在早期妊娠输卵管中表达的结果与免疫组织化学检测伞部的结果基本一致。mPGES-2 蛋白在壶腹部的免疫染色整体稍弱于伞部。

Western Blot was used to test the expression of NF-κB in nuclear and cytoplasm. The intranuclear expression of NF-κB was confirmed by ELISA assay. The influence on cell apoptosis of gastric carcinoma cell by Paeoniflorin and 5-FU was estimated by flow cytometry.

应用Western Blot方法检测各组胞核NF-κB表达情况，并应用胞核NF-κB活性检测进一步确定胞核内NF-κB的表达情况，流式细胞仪检测联合应用芍药苷及5-氟尿嘧啶(5-FU)对胃癌细胞凋亡的影响？

Although many efforts have been made to elucidate the role of Kail gene in many kinds of carcinoma,there are little reports concerning the role of Kail in human colorectal carcinoma,especially regarding the mechanism of its down-regulation in metastasis carcinoma. In present study,the expression of Kail was detected by in situ hybridization,immumohistochemistry and Western Blot in four cell lines of HCC with different metastatic potential.

为探讨Kai1基因与大肠癌转移之间的相关性，本研究利用四种不同转移潜能的大肠癌细胞系HCT116、HT29、LoVo与SW480，从mRNA与蛋白质水平，用原位核酸杂交、免疫组织化学与Western Blot三种方法检测Kai1／CD82的表达，得到了一致的结果：Kai1／CD82在HT29与SW480细胞系的表达较LoVo与HCT116为高。

The results indicated that Kail/CD82 is higher expressed in HT29 and SW480 than in LoVo and HCT116 whatever in mRNA or protein level.The possible mechanism of down regulation of Kail was also analyzed. By PCR-SSCP,8 exons of Kail,exon 3 to exon 10,were screened in LoVo and HCT116 cell lines. The methylation of CpG islands in promoter region was analyzed,and the expression level of p53 was detected by IH and Western Blot.

为进一步探讨Kai1基因表达下调的机制，本研究用PCR-SSCP技术分析了Kai1／CD82表达较低的LoVo与HCT116细胞系的Kai1基因第3～10外显子是否存在点突变，检测Kai1基因启动子区域CpG岛的甲基化状况，并用免疫组化与Western Blot等方法研究Kai1基因表达与p53异常之间的相关性。

The proteins from endosperm, root, stem, leaf, leafstalk of Jatropha curcas L. and their calli were hybridized the seed toxin protein of Jatropha curcas L. by Western blot.

然后我们以麻疯树的胚乳、根、茎、叶、叶柄及其诱导出的愈伤组织为材料，对麻疯树种子毒蛋白作Western-blot杂交。

At 0, 4, 8, 16, 24 h after operation, the expressions of SR-A protein and mRNA in liver tissue were assayed by western blot and RT-PCR respectively; the levels of plasma endotoxin and interleukin-6 (IL-6) were measured using limulus test and ELISA.

于术后0、4、8、16、24 h分别采用Western blot和RT-PCR检测肝组织中SR-A蛋白和基因表达水平；以鲎试验和ELISA测定血浆内毒素和白介素-6(IL-6)；光镜观察肝组织病理变化并测定血浆ALT、TB含量。

In this study, we found out that linalool, a small-molecule compound, could inhibit the growth of various human lymphocyte leukemia cell lines significantly. Meanwhile, it showed little toxicity to normal hematopoietic cells. To determine effects of linalool on the growth of a serious of human leukemia cell lines and normal hematopoietic cells and to explore its possible mechanisms, we employed various technologies, including MTT assay, Flow cytometry, Human Cancer PathwayFinder II Gene Array, Western Blot, RT-PCR and so on.

我们在筛选抗肿瘤小分子化合物时，发现天然小分子化合物芳樟醇能明显抑制多种人淋巴细胞白血病细胞增殖，而对正常人骨髓造血细胞及外周血细胞的增殖没有明显的影响，为此我们用MTT技术、流式细胞术、肿瘤相关信号通路分析技术以及Western blot、RT-PCR等手段和方法探讨了芳樟醇对不同类型白血病细胞系及相应正常造血细胞中的影响情况，及其诱导白血病细胞凋亡的可能分子机制。

COS-7-cell line grown to log phase was transfected using lipofectamine reagent. The expression level of β-NGF mRNA and the biological activity were analyzed by Northern blot and observation of neurite outgrowth of PC12 cell line stimulated by supernatant, respectively.

利用脂质体 Lipofectamine方法转染 COS-7细胞，对阳性克隆 COS-7细胞的 mRNA和培养上清分别进行 Northern blot分析和观察对 PC12细胞突起生长的作用。

After two hours of the mice leaveing warming boxes, 5 mice randomly selected from each group per timesection were executed by cervical vertebra luxation. The HSP70 of embryonic brain, protein content and deformations of embryonic nervous systems were quantitatively analyzed with the Western dot blot and Lowry method.

移出温箱2h后，每组每时段随机取5只脱颈法处死，采用Western dot blot 和lowry方法定量分析胚胎脑HSP70和蛋白质含量与胚胎神经系统畸形。

Methods The stablishment of lymphoblast cell lines was by EBV-immortalization and subtyping of the cell lines by cell fusion, mytomycin C inhibition analysis and/or immunoprecipitation and Westemn blot.

方法将我国3例FA患者外周血细胞用EB病毒活化法建立原始B-淋巴细胞系，采用丝裂霉素生长抑制试验和细胞融合后MMC抑制试验的互补试验进行分型；利用单克隆抗体免疫沉淀法和Western blot法进行分型。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。