查询词典 blot

Methods The methods of both morphological analysis and cell viabilities were used, and Western blot was also used to analyce the role of antitoxiciy of estrone.

用形态学鉴定和细胞死亡率的测定以及Western blot方法分析雌激素对NMDA诱导神经元凋亡的作用。

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

The 528 bp fragment of flic gene was successfully cloned into vector, the pGEX-flic recombinant plasmid was expressed and induced by IPTG and Western-blot method showed good antigenicity of the recombinant protein, and there were flexible regions such as coil region and turn region in flic protein.

获得的目的基因片段长度为528 bp，测序表明其序列与GenBank公布的一致；软件预测结果显示，该蛋白具有多个明显的二级结构成分及跨膜区；SDS-PAGE检测表明，在约50 ku处有一特异性条带；Western-blot检测表明，表达蛋白具有良好的抗原活性。

Coli mainly in a form of inclusion body. The expressed product contained about 25% of total somatic protein and showed good reactogenicity as proved by Western blot. The IgG level against GAMP induced by GAMP-CRM197 conjugate was significantly higher than those by GAMP and by the mixture of CAMP and rCRM197. Repeated immunization with the conjugate induced immunopotentiation, indicating the effect of CRM197 as a carrier protein.

结果重组CRM197在大肠杆菌中主要以包涵体形式表达，表达量占菌体总蛋白的25%左右；Western blot证明重组rCRM197具有良好的反应原性；各针接种后，结合物诱生的多糖特异性IgG水平均显著高于GAMP组和GAMP+rCRM197混合物组，具有较强的免疫原性；多次接种产生了免疫增强效应，重组CRM197具有载体蛋白的作用。

The study was purposed to investigate the effect of extract of Agkistrodon Halys venom on proliferation and apoptosis of K562 cells. The inhibition of K562 cell proliferation was measured by MTT assay; The morphologic changes of K562 cells was observed by microscopy; the apoptosis of K562 cells was measured by flow cytometry; the activity of extracellular signal-regulated kinase in K562 cells was detected by Western blot.

本研究探讨皖南蝮蛇毒蛋白提取物对白血病细胞K562增殖的影响及诱导凋亡的作用，采用MTT法检测不同浓度的蛇毒蛋白提取物对K562细胞生长的影响；应用电子显微镜观察药物作用后K562细胞形态的改变；流式细胞术检测蛋白提取物对K562细胞细胞凋亡作用；Western blot检测细胞增殖酶活性的变化。

Methods: Human colon cancer cell line DLD1 was treated with Bcl-XL siRNA, The protein level of Bcl-XL in the cells was determined by Western blot; the apoptotic ratio and survival condition were detected by flow cytometry assay and trypan blue-stained cell counting with hemocytometer, respectively. Results: The level of Bcl-XL protein in DLD1 cells was obviously down-regulated by Bcl-XL siRNA.

采用人结肠癌细胞株DLD1，利用人工合成的Bcl-XL靶向小分子干扰RNA转染DLD1细胞，通过Western blot法检测转染细胞Bcl-XL蛋白的表达，流式细胞仪检测处理后细胞的凋亡情况，锥虫蓝染色法计数存活细胞。

Western blot analysis showed that MdLys was highly expressed in midgut and was also detected in the hemolymph and fat body.

在蛋白水平上通过Western Blot 分析发现，该基因在中肠中大量表达，在脂肪体和血淋巴中也可以检测到。

At embryonic day 14, MEFs were recovered from the embryos and cultured under the selection of HAT (0.016 mg of hypoxanthine/ml, 0.01 mmol/L aminopterin, 0.0048 mg of thymidine thymidine/ml), and the cell cycle, apoptosis, and the relative protein expressions were analyzed by flow cytometry and Western blot.

用不同剂量的电离辐射或紫外光分别照射ES或EF细胞，然后利用流式细胞仪检测该突变对小鼠ES细胞周期及对ES和EF细胞凋亡的影响，并利用Western blot检测相关蛋白的表达。

SDS-PAGE and Western Blot analyses showed that target protein was expressed mainly in the form of inclusion body.

经SDS-PAGE和Western Blot分析，四价抗体主要以不溶性的包涵体的形式表达。

SDS-PAGE and Western Blot analysis showed that scFv was expressed mainly as inclusion body in the cytoplasm of E.

经SDS-PAGE和Western Blot分析，单链抗体主要以包涵体的形式在大肠杆菌中表达。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。