查询词典 blot

Coli genome was amplified by using PCR method. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the Mn-SOD protein expressed by pET-28a vector and purified was injected into rabbits to prepare polyclonal antibody. Western-blot was used to detect the effect of polyclonal antibody.

coli基因组中扩增Mn-SOD基因编码区，将它克隆到原核表达载体上进行大量表达和纯化，再用纯化的蛋白对新西兰大白兔进行背部多点注射，40d后取其血清，用Western-blot印迹实验测定抗体效果。

Western Blot analysis also demonstrated that level of ANXA1 in the villus was up-regulated.

Western Blot分析ANXA1蛋白质的表达，结果与RT-PCR一致。

Results of Western-blot reveal: along with the increase concentration of taxol, the expressions of Fas、FasL proteins increased gradually, while the expressions of Caspase-8、Caspase-3 zymogen proteins decreased gradually.

Western-blot结果显示：随着紫杉醇浓度的增加，Fas、FasL蛋白表达量逐渐增加，Caspase-8、Caspase-3酶原蛋白表达量逐渐减少。

Methods Using Western ligand blot technique, the authors measured plasma levels of IGFBP-1-4 in uremic acidotic rats and uremic but without acidotic rats, in the non-uremic acidotic and pair-fed rats and also delermined liver mRNA in UA and UB rats.

采用Western ligand blot等方法，对随意进食组、尿毒症酸中毒组及其对照组尿毒症未合并酸中毒组、非尿毒症酸中毒组及其对照组无酸中毒组的大白鼠进行了血清IGFBP-1-4的测定，对UA、UB组肝脏IGFBPs mRNA进行了测定。

Methods 0, 2.5, 5, 10, 15 and 20 μmol/L arsenious acid-treated human hepatocellular carcinoma HepG2 cells were cultured under hypoxia for 8 hours. The cell proliferation and cell viability was assayed by WST-8 and the trypan blue dye exclusion method. The expressions of HIF-1α in human hepatocellular carcinoma HepG2 cells were checked by RT-PCR and Western Blot.

以0、0.25、0.5、1、2和4μmol/L的亚砷酸处理人肝癌细胞HepG2缺氧环境中培养8h；1μmol/L的亚砷酸处理人肝癌细胞HepG2缺氧培养0~10h，WST-8法和台盼蓝染色法分析细胞增殖和活力，采用RT-PCR和Western Blot方法检测肝癌细胞中HIF-1α的表达。

Results Bestain could significantly lower the serum DHBV level. ALT and AST in serum were reduced during the treatment. Examination of DHBV DNA in liver with Southern Blot indicated Bestain could inhibit DHBV DNA replication.

结果 乌苯美司用药1月能使血清DHBV DNA总体水平显著降低；治疗期间同一时相内，能使血清ALT明显降低；Southern Blot检测肝组织内DHBV DNA，其结果提示该药能抑制病毒在肝内的复制。

Ap with hyperlipaemia model was prepared by both triton wr1339 and cerulein simultaneously. human albumin was used to treat the ap accompanied with hyperlipaemia. in each group, the histological score, volume of ascites, ratio of pancreatic wet/dry weigh, serum amylase and pancreatic acinar cell apoptosis in each group were compared. the level of protein kinase c membrane translocation in pancreatic tissue was detected by western blot.

分别用triton wr1339、cerulein制作大鼠高脂血症和急性胰腺炎（acute pancreatitis，ap）模型，同时应用两者制作伴有高脂血症的ap模型，应用白蛋白治疗伴有高脂血症的ap，比较各组胰腺病理损害评分、腹水量、胰腺湿/干比、血清淀粉酶和胰腺组织的凋亡；western blot检测胰腺组织pkc的膜转位。

Methods Hyperlipaemia and AP model were established with Triton WR1339 and Cerulein respectively. AP with hyperlipaemia model was prepared by both Triton WR1339 and cerulein simultaneously. Human albumin was used to treat the AP accompanied with hyperlipaemia. In each group, the histological score, volume of ascites, ratio of pancreatic wet/dry weigh, serum amylase and pancreatic acinar cell apoptosis in each group were compared. The level of protein kinase C membrane translocation in pancreatic tissue was detected by Western blot.

分别用Triton WR1339、Cerulein制作大鼠高脂血症和急性胰腺炎（acute pancreatitis，AP）模型，同时应用两者制作伴有高脂血症的AP模型，应用白蛋白治疗伴有高脂血症的AP，比较各组胰腺病理损害评分、腹水量、胰腺湿/干比、血清淀粉酶和胰腺组织的凋亡；Western blot检测胰腺组织PKC的膜转位。

Methods RT-PCR, Western blot were used to detect the expression of bax in human gastric cancer cells line SGC-7901, and observe the effect of rhIL-24 on chorioallantoic membrane.

采用RT-PCR法和Western blot法检测rhIL-24对胃癌细胞株SGC-7901中促凋亡因子bax表达的影响，鸡胚绒毛尿囊膜技术观察其对血管形成的影响。

As the DDP group A549DDP cells were treated with IC10 of cisplatin for 48 hours. As the DDP＋Rh2 group A549DDP cells were treated with IC10 of cisplatin and IC5 of gensenoside Rh2 for 48 hours. As the controlling group A549DDP cells were not treated with any kinds of drugs. Changes in cellular morphology were observed by fluorescence microscopy. Peak value apoptosis of A549DDP cells were inspected by flow cytometry. State of PTP was evaluated by ultraviolate spectrofluorometer. Changs of calcium in cells and membrane potential of mitochondrion were determined by flow cytometry. Release of cyt-c from mitochondrion with westton blot Radiative activity of〓Tc-MIBI ingested by cells was measured withγcounter. Results: IC50 of cisplatine to A549 cells was 24uM.

以无毒浓度的Rh2单独作用于A549DDP细胞作为Rh2组，以低效浓度的顺铂单独作用于A549DDP细胞作为DDP组，以无毒浓度的Rh2和低效浓度的顺铂联合作用于A549DDP细胞作为Rh2+DDP组，作用时间为48小时，以不加药物干预作为对照组，Hoechst33258染色荧光显微镜下观察细胞形态的变化，流式细胞仪检测细胞凋亡峰；紫外光分光光度计检测线粒体PTP开放情况，流式细胞仪检测细胞内钙离子浓度及线粒体跨膜电位的变化，Westton blot检测线粒体内细胞色素C释放情况及Caspase-3的活性，γ计数仪检测细胞摄取〓Tc-MIBI放射性活性。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。