查询词典 blot

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

Equal amount of absolute alcohol was given to the control group. The expressions of vitamin D receptor in HL-60 cells of each group were identified by Western blot analysis, and cellular morphological changes were observed after being stained by Wright-Giemsa.

Western印迹技术鉴定维生素D受体在各组HL-60细胞的表达；Wright-Giemsa染色观察细胞形态学变化；流式细胞术检测细胞表面标志物CDl4抗原的表达；反转录-聚合酶链反应法分析c-myc基因的表达。

Methods Serum IGFBP-3 levels in 20 normal adults, 33 active acromegaly and 34 patients with idiopathic growth hormone deficiency were measured by Western blot.

本文采用Western印迹方法测定20例正常人，33例活动性肢端肥大症病人和34例特发性生长激素缺乏症病人血清IGFBP-3水平。

Western blot and double staining analyses show NYD-SP27 becomes detached from sperm, as they undergo capacitation and acrosome reaction.

Western blot和双染的研究表明在精子发生获能和顶体反应的过程中NYD-SP27逐渐从精子上丢失。

Methods The dot immunogold filtration assay kit was developed and potato agglutinin was applied to blot blood quickly.

摘要目的？摇建立一种检测全血标本的棘球蚴病现场快速诊断方法。

It was found that the fusion protein has good antigenicity by Western-blot.

Western印迹分析结果说明该基因在大肠杆菌中的融合表达产物rSjGcp具有良好的抗原性。

Western blot was used to identify the antigenicity and immunoreactivity of rFimA.

采用Western blot鉴定rFimA抗原性和免疫反应性。

Its antigenicity was identified by Western blot.

Western blot鉴定重组HpaA蛋白的抗原性。

The antiserum can be used for Western blot.

所得的抗血清可用于后续的蛋白检测。

The titer and specificity of murine antiserum was evaluated by ELISA, Western blot and immunofluorescent staining.

用ELISA、Western blot和免疫荧光分别检测抗血清的效价和特异性。

His female bullfighter Lydia gradually Health love, but she was in a vegetative state after the Bulls Zhuangcheng away from her, just because Lydia's ex-boyfriend showed more affectionate than he is like love.

他对女斗牛士莉迪亚渐生爱意，却在她被公牛撞成植物人后离她而去，仅仅因为莉迪亚的前男友表现出好似比他更深情的爱。

I know of none which are not easy to answer, but what I am trying to do now is only by a series of hints to throw some light on the narrow path of legal doctrine, and upon two pitfalls which, as it seems to me, lie perilously near to it.

我不认为有什么难以回答的问题，但我现在要做的仅仅是通过一系列线索，来给法律理论的狭窄路径以及在我看来离它近得危险的两个陷阱提供一些启示。