查询词典 blot

This character is used in this study to develop three Western blot protein markers.

在本论文中，这种特点被用于制备三种western blot蛋白分子量标准。

The expressed product was purified by metal chelate chromatography, identified by Western blot and determined for activity.

表达产物经金属螯合层析柱纯化，并进行Western blot 分析及活性检测。

The expressed products were purified by Glutathion-Sepnarose chelation affinity chromatography and tested by western blot.

对表达的蛋白进行Western blot分析和谷胱甘肽琼脂糖亲和层析纯化，得到纯化的VP4融合蛋白。

OBJECTIVE: Western-blot technique is utilized to further validate FHL1 and HOXD13 gene expression in protein levels in muscle tissue of patients with congenital clubfoot, the in vivo HOXD13 and predicted binding sites in embryonic foot development was verified using chromatin immunoprecipitation technology.

目的：应用Western-blot技术进一步验证先天性马蹄内翻足患者足部肌肉组织中FHL1，HOXD13基因蛋白质水平的表达，应用染色质免疫沉淀技术验证在胚胎足部发育时在体内HOXD13和预测结合位点的结合作用。

The eyes were periodically examined by retinoscopy, and A-scan ultrasonography. Two weeks later, the eyeballs were removed and the apoptotic cells were determined by electron microscopy, TUNEL technique and flow cytometry in scleras. Both bFGF and the bcl-2 protein were determined by the immunohistochemisty method and the Western-blot method. Results: All eyes treated with -10.00D or -20.00D concave lens produced defocus-induced myopia.

实验前后用睫状肌麻痹下验光、A超动态观察豚鼠眼屈光度和眼轴长度，并取后极部巩膜组织，用电镜、终末脱氧核糖核苷酸转移酶介导原位缺口末端标记法和流式细胞术检测巩膜的凋亡细胞，应用免疫组织化学染色和蛋白印迹（Western-blot）法检测bFGF和bcl-2蛋白。

Third, the prepared McAbs were identified by cytochemistry, specificity and Western-blot.

然后用细胞化学、特异性、Western-blot等方法鉴定制备抗体的免疫反应及特异性。

Methods: We used virus proliferation assay cell viability assay to evaluate the proliferation and cytolysis selectivity of CNHK500. And we used Western-blot to confirm the expression of adenovirus CNHK500 E1A and E1B in cancer and normal cells.

行病毒增殖实验和细胞生长抑制实验，验证CNHK500选择性复制和杀伤能力；Western blot检测腺病毒E1A和E1B在细胞中的表达。

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

Some of the differential expression proteins were verified by Western blot analysis and immunohistochemical staining, and the results were consistent with 2-DE analysis.

一些差异表达的蛋白质通过Western blot分析和免疫组织化学染色进行了验证，结果和2-DE分析的相一致。

The expression of GDNF of spinal L4 DRG was determined by Western blot.

采用Western blot法检测脊髓L4背根神经节GDNF的蛋白表达。

Results showed that 0.01g/mL extract of Oxalis corniculata L. had obvious antimicrobial effect on S. aureus, but all extract of Oxalis corniculata L has not any antimicrobial effect on E.

结果表明，0.01g/ml的提取物对摇床培养的金黄色葡萄球菌生长具有明显的抑制作用；但各种浓度的提取物对摇床培养的大肠杆菌生长均无抑制作用。

This will be totally different from Zaro's texture mod, it will be desaturated, dirty etc.

这会和Zaro的那个材质补丁有很大不同，这边是更多的不饱和色，整个都灰扑扑的。