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SDSPAGE and Western blot analysis showed that HSA/M2e was successfully expressed in the culture medium with an apparent molecular weight of 68 kD.

SDSPAGE和Western印迹分析证实了甲醇诱导的培养基上清中含有HSA/M2e,分子量约为68 kD。

His achievements were blot ted out at one stroke.

他的功绩被一笔抹杀。

A novel gene CCR (Cell-Cycle Related) was cloned from Atriplex hortensis. Northern blot analysis showed that the CCR gene specifically expressed during G1 and early S phase of the cell cycle, but suppressed by ABA treatment.

从山菠菜和拟南芥中克隆了一个新的未知功能的植物基因,编码DNA结合蛋白,其在细胞周期的G1和S期特异表达,受ABA抑制,命名为CCR(Cell-CycleRelated)。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子,以色谱法分离纯化后进行体外活性研究,构建表达载体pPICZαA- ALR,经电穿孔转入毕赤酵母中,用0.5%甲醇诱导表达;重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明, rhALR以分子量为30kD的二聚体为主;定量分析结果表明,重组酵母培养上清中rhALR约占总蛋白的66%,表达量约为40mg/L;经DEAE柱和G75柱纯化后,获得的rhALR纯度大于95%,得率为52%;体外生物学活性实验表明,rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Western blot analysis shows that the protein could be identified only by the antiserum to M. bovis but not the sera against.M, paratuberculosis, brucellosis, babesia and infectious bovine rhinotracheitis virus.

Western blot分析显示:该融合蛋白能与抗牛分枝杆菌阳性血清发生特异性反应,而与牛副结核病、牛布氏杆菌病、牛巴贝斯病和牛传染性鼻气管炎病阳性血清不反应。

Westem blot result: Bax in endochylema and bioblast of Hep-2 cell express augment in the experiment group, Bcl-2 in endochylema express diminutus, cytochrome C in endochylema express augment obviously, cytochrome C in bioblast express diminutus obviously, p20 in endochylema express augment obviously.

说明肿瘤抑素19肽诱导喉癌Hep-2细胞凋亡通过线粒体途径。

You should blot out of all your terrible memories.

你必须在头脑中去除这些可怕的记忆。

He tried to blot out the image of Helen's sad face.

他尽量不去想海伦的那张忧伤的脸。

And Jehovah said to Moses, Whoever has sinned against Me, I will blot him out of My book.

32:33 耶和华对摩西说,谁得罪我,我就从我的册上涂抹谁的名。

I closed my eyes and tried to blot out the events that happened.

我闭上眼睛,试图忘却一些发生过的事情。

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推荐网络例句

Warden: That's a beautiful dog, but don't bring her again.

那是条漂亮的狗,但是不要再把她带来了。

You get up in the morning – no hangover!

你第二天早上起来的时候—不会宿醉!

Usually, the whitest teeth are the two front teeth while molars are generally two shades darker.

通常,雪白的牙齿是两个战线同时臼齿齿通常有两种色系黑暗。