查询词典 blot

Methods The expression of KLF6 mRNA and protein in 23 tissue specimens of HCC and adjacent normal tissues by reverse transcription-polymerase chain reaction and Western blot respectively.

分别以逆转录聚合酶链反应和Wstern blot检测原发性肝癌及其癌旁组织KLF6mRNA及蛋白质的表达水平。

TNFR1/RANK chimera cloning of cDNA, with its plat E-retroviral package by retroviral infection such transfer to TNFR1/R2 gene knock-out mice (TNFR1-/R2-) Bone marrow macrophages in; TNFɑ used to stimulate, respectively, to observe the OC and differentiation of bone re-absorption capacity; confirmed by Western Blot method TNFR1/RANK through NF-?

克隆TNFR1/RANK嵌合体的cDNA，用plat E将其包装成逆转录病毒，通过感染将这种逆转录病毒转染到敲除TNFR1/R2基因的小鼠(TNFR1-/R2-)骨髓巨噬细胞中；用TNFɑ刺激，分别观察OC的分化及骨的重吸收能力；用Western Blot方法证实TNFR1/RANK是通过NF-？

Perceptively on 3d and 5d. Collagen is positive in Western blot on 9d. Aniline dyeing is positive on 12d. The control group was negative. Ad-BMP-2 transferred MSCs cultured in fibrin gel was positive in Aniline dyeing, Immunohistochemical. The therapy effect of group was better in histology, biochemistry and biomechanics in all periods than the other two groups, and was similar to the natural cartilage.

结果 ①Ad-BMP-2转基因MSC BMP-2、CollagenⅡ的PT-PCR分别在3、5d呈阳性，CollagenⅡ的Western Blot 9d为阳性，较对照组有显著差异；②转基因MSC经纤维蛋白体外三维培养后免疫组化和苯胺蓝染色呈阳性，电镜显示细胞生长良好，有基质合成；③转基因修复组各时期大体、组织形态学和组织化学成份均显著优于其它两组，12W时力学和组织学已接近正常关节软骨。

Marked changes of apoptosis including condensation of chromatin and nuclear fragmentation were observed very clearly by Hoechst 33258 fluorescence staining and a characteristic "ladder" of DNA fragments was elicited by agarose gel electrophoresis; Western blot analysis revealed that caspase-3 was activated by the loss of caspase-3 proenzyme (32kDa) and the appearance of its 20kDa subunit, and that along with the apoptotic process caspase-3 activity was increased concurrently.

Hoechst 33258荧光染色观察到典型的核浓缩、核碎裂等细胞凋亡的形态学变化，琼脂糖凝胶电泳观察到细胞凋亡时的DNA"梯形"条带；Western blot检测结果表明32kDa的caspase-3酶原被激活，出现20kDa的亚单位活化片段，同时在细胞凋亡过程中，caspase-3活性显著增高。

Methods Mouse macrophages were stimulated with Mpe LAMPs to analyze the activitation of NF-κB, and the effect of pyrrolidine dithiocarbamate, an inhibitor of NF-κB, on NF-κB activation was also studied by indirect immunofluorescence and western blot.

用从穿透支原体中提取的脂质相关膜蛋白刺激小鼠巨噬细胞，经Western blot和间接免疫荧光等方法检测NF-κB是否被激活，以及NF-κB抑制剂PDTC对NF-κB活性的影响。

Methods Forty women and their babies (40) were enrolled in this study. Placental tissues were assayed for leptin mRNA by reverse transcription/polymerase chain reaction, and assayed for the obese gene protein leptin by Western-blot and immunohistochemistry. Blood was taken from the umbilical cord of the babies at delivery. Serum leptin was measured by radio-immunoassay. Neonatal anthropometric measurements were recorded within 48 hours after delivery.

方法在40例产妇分娩时采集胎盘与脐血，采用逆转录聚合酶链反应检测胎盘肥胖基因mRNA相对表达水平，采用Western-Blot检测胎盘肥胖基因蛋白表达水平，采用免疫组化观察胎盘肥胖基因蛋白表达位点，采用放射免疫法检测脐血瘦素水平，采用Ponderal指数［PI=100×体重/身长3］估测新生儿营养状态。

In addition,the expression of Galβ-1,4-GlcNAc group was studied by ricinus communis agglutinin-I lectin blot and lectin histochemistry with RCA-I respectively.

并利用特异识别-β1，4-半乳糖苷键的蓖麻凝集素-Ⅰ，经letin blot和组织化学方法分析-β1，4-半乳糖苷键在各型人脑星形胶质细胞瘤中的表达变化和表达定位。

The expressions of RhoA, ROCK1/2 and COL Ⅱ in the induced BMSCs and after application of enzyme inhibitor Y27632 were detected by Western-blot and BT-PCR.

采用Western-blot和RT-PCR方法分别检测RhoA和ROCK1/2Ⅱ型胶原在细胞分化过程中的表达及应用Rho激酶抑制剂Y276532后的表达。

Nuclear Run-on Assay was used to address the contribution of transcriptioninitiation to the regulation of five histones by YSV. Actinomycin D inhibitor studies were used to detect the mRNA half-life of five histones. SLBP weremeasured by Western blot assay to identify regulation of YSV on Pre-mRNAsplicing.

应用Nuclear Run-on Assay观察YSV对人肝癌BEL-7402细胞的五种组蛋白转录效率的影响；应用放线菌素D转录抑制法观察YSV对组蛋白mRNA半衰期的影响；应用Western blot检测SLBP的表达水平观察YSV对组蛋白mRNA加工的影响。

SDS-PAGE and Western blot of culture supernatant showed that hsIL-1RⅠ was secretively expressed. Recptor-ligand binding assays showed that the recombinant hsIL-1RⅠ has receptorligand binding activity.

发酵液经SDS-PAGE和Western blot证实分泌表达了sIL-1RI，经蛋白印迹受体配基结合反应证实分泌表达的sIL-1RI具有配基受体结合的生物学活性。

Results showed that 0.01g/mL extract of Oxalis corniculata L. had obvious antimicrobial effect on S. aureus, but all extract of Oxalis corniculata L has not any antimicrobial effect on E.

结果表明，0.01g/ml的提取物对摇床培养的金黄色葡萄球菌生长具有明显的抑制作用；但各种浓度的提取物对摇床培养的大肠杆菌生长均无抑制作用。

This will be totally different from Zaro's texture mod, it will be desaturated, dirty etc.

这会和Zaro的那个材质补丁有很大不同，这边是更多的不饱和色，整个都灰扑扑的。