查询词典 blot

Our results demonstrate: The structures of the organs are normal, and the shapes of cells are clearly visible. There are lots of positive brown granules in Chief cells and Parietal cells in abomasum as well as the mucosa epithelial cells and gland cells of duodenum. Three bands with a molecular mass close to 120KDa、110KD and 98KDa were identified by Western Blot. The Ob-R levels of 120KDa in abomasum were significantly higher than that of in small intestine. The levels of 110KDa were similar in the two organs. The expression of 98KDa Ob-R was weak.

HE染色结果显示，各组织结构正常，细胞形态清晰可见；免疫组织化学SABC染色显示，在皱胃胃体部固有层胃底腺的主细胞和壁细胞及十二指肠黏膜上皮细胞和固有层肠腺的柱状细胞中均可见大小数量不等的棕黄色颗粒；western Blot 实验发现，在胃和小肠均检测到120KDa、110KDa和98KDa三条带。120KDa长型瘦素受体蛋白在胃中表达量显著高于小肠中的表达；110KDa的短型瘦素受体蛋白，在小肠和皱胃中表达量接近。98KDa短型受体蛋白在胃和小肠表达均较弱。

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

Western blot and double staining analyses show NYD-SP27 becomes detached from sperm, as they undergo capacitation and acrosome reaction.

Western blot和双染的研究表明在精子发生获能和顶体反应的过程中NYD-SP27逐渐从精子上丢失。

Western blot was used to identify the antigenicity and immunoreactivity of rFimA.

采用Western blot鉴定rFimA抗原性和免疫反应性。

Its antigenicity was identified by Western blot.

Western blot鉴定重组HpaA蛋白的抗原性。

The titer and specificity of murine antiserum was evaluated by ELISA, Western blot and immunofluorescent staining.

用ELISA、Western blot和免疫荧光分别检测抗血清的效价和特异性。

Expression of Caspase-3 and bcl-2 protein In E20 cerebral cortex, the expression of cleaved Caspase-3 in the encephalocoele group are higher than that in control group, and the expression of bcl-2 in the encephalocoele group are lower than that in control group. These findings were consistent with the results of the western blot analysis in E14 Brain.

四、Western-blot检测E14、E20胎鼠大脑皮质中caspase-3、bcl-2蛋白的表达 1、用Western-blot方法检测不同组E20胎鼠大脑皮质中caspase-3、bcl-2蛋白表达水平，结果表明对照组、给药无畸形组、脊柱裂组胎鼠大脑皮质中caspase-3、bcl-2蛋白表达水平无明显改变，而脑膨出组胎鼠大脑皮质中caspase-3蛋白活化片段表达水平明显增加，bcl-2蛋白表达水平明显减少。

Results The Southern blot result showed that 4 among 188 goats produced by pronuclear microinjection had integrated hEPO gene.

结果在原核注射获得的188头羔羊中，经Southern blot法检测有4头羊含有hEPO基因，其中3头为母羊，1头公羊于出生后20d死亡；3头转基因母羊hEPO基因的拷贝数分别为1、10、2；Western blot检测结果显示转基因羊乳中的hEPO分子质量为32kDa；MTT法检测结果表明，在泌乳10d的3只转基因羊乳汁中，每毫升乳汁中hEPO活性分别达到1.17×102IU、1.90×104IU、1.91×104IU。

In vivo, CXCR4-A549 and A549 cells were injected subcutaneously into nude mice(each group in 6).Four weeks later the mice were scarified and the tumors in situ and the lungs were taken out to be examined histologically.(3) Th cell cycle distribution was measured by flow cytometry to analyse the effect of SDF-1/CXCR4 in the cell proliferation and atoposis. The changes of the intracellular calcium concentration after SDF-1 activation were aslo measured by flow cytometry. Western blot was used to anlayse the phosphorylation of AKT and ERK, and the expression of MMP-2 and VEGF-C.

3为探讨SDF-1/CXCR4在肺癌侵袭和转移中的作用机制：①利用流式细胞仪检测CXCR4-A549与A549两组细胞的增殖和凋亡，分析上调CXCR4的表达后，对细胞生长周期的影响；②利用流式细胞仪检测两组细胞钙离子内流强度，分析SDF-1/CXCR4对钙离子通道活性的调控；③采用Western blot实验检测两组细胞PI3K/AKT、MAPK/ERK相关信号通路的活性，分析SDF-1/CXCR4可能激活的下游信号分子；④采用Western blot和RT-PCR检测两组细胞肿瘤转移相关基因VEGF-C、MMP-2的表达，探讨SDF-1/CXCR4与VEGF-C、MMP-2的相互作用关系。

Northern blot analysis showed that the GFP gene was correctly expressed in the transgenic plants. Green fluorescence was observed in roots and immature embryos of transgenic plants by fluorescence microscopy.Progeny analysis showed that GFP insertions were inherited in T_2 generation with normal expression.Both Mendelian and non-Mendelian inheritances were observed in T_1 progenies of transgnic maize,and non-Mendelian inheritances were observed in T_2 progenies.

Southern blot结果表明GFP基因以低拷贝数、简单的方式(1～3条Southern杂交带)整合到玉米基因组；Northern blot结果表明GFP基因在转录水平上表达；在转基因植株的根和幼胚中观察到GFP表达；转基因植株的后代遗传分析表明GFP基因能够遗传至T_2代并正常表达，部分转基因株系中的GFP基因以孟德尔遗传方式传递给T_1代，但T_2代不符合孟德尔分离比。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。