查询词典 blot

Northern blot and dot blot hybridizations indicate that it is expressed ubiquitously in human 50 tissues and 7 cancer cell lines.

Northern杂交和点杂交分析显示，该基因以大约5.4kb的单一转录物广泛表达于人体各种组织，而且在人部分肿瘤细胞中高表达。

The expression level and enzymic activity of transgenic rice were evaluated by Northern blot, Western blot and the enzyme reaction.

用Northern blot ，Western blot和酶活测定的方法检测了转基因水稻中几丁质酶和葡聚糖酶的表达和活性。

Sequencing analysis and database searches indicated that there were 19 known genes and 31 unknown cDNA fragments in the sequenced 72 dot blot positive clones specific for gastrula embryos,and 52 known genes and 37 unknown cDNA fragments in the sequenced 98 dot blot positive clones specific for tail bud embryos.

测序和基因数据库比对结果表明，72个原肠期斑点杂交阳性克隆中，包括19个已知基因的cDNA片段和31个没有同源性的cDNA片段；98个尾芽期斑点杂交阳性克隆中，包括52个已知基因的cDNA片段和37个没有同源性的cDNA片段。

METHODS:Hind limbs ischemia was made by clamping infrarenal aorta with a microvascular clip and lung injury was made by following reperfusion. Lung tissue was obtained from the animals subjected to sham operation, 4 h ischemia without reperfusion and 4 h, 8 h, 16 h, 24 h, 48 h reperfusion following 4 h ischemia. The levels of HO-1 mRNA and protein were measured at different times by Northern blot and Western blot.

采用夹闭大鼠腹主动脉下段造成双下肢缺血和再灌注性肺损伤模型，分别采集假手术组，缺血4 h组及缺血4 h再灌注4、8、16、24、48 h组肺组织标本，以Northern印迹、Western印迹及免疫组化分析，观察HO-1表达的变化。

The expression product was analyzed and its antigenicity was further studied by ELISA and Wstern blot method with chimeric protein as coating antigen.we used chimeric protein as coating antigen to test anti HBs with ELISA and Wstern blot in 58 seral samples.

通过ELISA和Western blot检测表达产物的抗原性，并以表达产物为包被抗原，通过ELISA和Western blot检测58份乙肝患者血清抗 HBs。

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。

The livers of sd bandicoots were infected by raav. at the 2nd month and 9th month, southern blot assay and dot blot assay were used to detect the integrated gene and mrna of hcv core protein and the titer of the raav respectively.

重组腺伴随病毒感染大鼠肝脏，感染2， 9 mo后分别以southern blot印迹杂交法和dot blot杂交法检测鼠肝hcv core区基因的整合、mrna形成情况及病毒滴度。

We verified the purification procedure by SDS-PAGE and Western blot. Their interactions were investigated by using Far-Western blot and Pull-down assay.

通过葡萄球菌V8蛋白酶和溴化氰对P4.2蛋白的限制性酶切分析，我们选取了一系列序列重叠的P4.2片段进行克隆表达。

Oocyte samples from one group were collected to detect the presence and integration of HBV DNA within cells and chromosomes using PCR, Southern blot, dot hybridization and fluorescence in situ hybridization. The female animals from another group were mated with their normal males, respectively. Their zygotes, 2-cell embryos were collected to detect the integration of HBV DNA in the female pronuclei of zygotes and the replication and expression of HBV genes in the 2-cell embryos using FISH, RT-PCR and immunofluoresence assay.(1) PCR detected positive bands in the tested oocyte samples fromgoldon hamster and mice. Southern blot revealed clear hybridization signals in PCR products.

研究用金黄地鼠和小鼠建立实验动物模型：将卵巢内注射HBV DNA的实验动物分成两组，一组注射后进行超排卵，收集卵巢和输卵管的卵母细胞，用PCR、Southern杂交，斑点杂交和荧光原位杂交(fluorescence in situ hybridization、FISH)检测HBV在卵母细胞内的存在和染色体上的整合；另一组超排雌鼠与正常雄鼠合笼，收集受精卵和2-细胞胚，用FISH、RT-PCR和免疫荧光检测技术分别研究HBV基因在受精卵雌原核上的整合以及在2-细胞胚中的复制与表达。

Our results demonstrate: The structures of the organs are normal, and the shapes of cells are clearly visible. There are lots of positive brown granules in Chief cells and Parietal cells in abomasum as well as the mucosa epithelial cells and gland cells of duodenum. Three bands with a molecular mass close to 120KDa、110KD and 98KDa were identified by Western Blot. The Ob-R levels of 120KDa in abomasum were significantly higher than that of in small intestine. The levels of 110KDa were similar in the two organs. The expression of 98KDa Ob-R was weak.

HE染色结果显示，各组织结构正常，细胞形态清晰可见；免疫组织化学SABC染色显示，在皱胃胃体部固有层胃底腺的主细胞和壁细胞及十二指肠黏膜上皮细胞和固有层肠腺的柱状细胞中均可见大小数量不等的棕黄色颗粒；western Blot 实验发现，在胃和小肠均检测到120KDa、110KDa和98KDa三条带。120KDa长型瘦素受体蛋白在胃中表达量显著高于小肠中的表达；110KDa的短型瘦素受体蛋白，在小肠和皱胃中表达量接近。98KDa短型受体蛋白在胃和小肠表达均较弱。

World War II: Battle of Saipan - Americans take Saipan.

1944年的今天，美国在塞班战役中拿下塞班岛。

When the scorner, the rejecter of truth, has become presumptuous; when the routine of work in the various money-making lines is carried on without regard to principle; when the student is eagerly seeking knowledge of everything but his Bible, Christ comes as a thief.{DA 635.3

当讥诮和拒绝真理的人，到了任意妄为时，当追求财利者罔顾按道义经营谋利时，当学人正在兢兢业业地研究各种知识，而单把圣经丢在一边时，基督要像贼一样来临。