筛选法

Then biotransformation of trans-anethole to anisaldehyde or anisic acid with these strains were carried out by fermentation. The contents of trans-anethole,anisaldehyde and anisic acid in the culture broth were analyzed by the methods of TLC,photometry and HPLC determined.

通过富集培养的方法，从八角树下土壤中筛选能将反式茴脑转化生成茴香醛或茴香酸的细菌菌株，采用薄层层析法、分光光度法和HPLC法相结合对转化液进行分析。

Secondly,the Popescu method was adopted to do further screening by virtue of linear correlation coefficient of regression calculation.

首先分析反应发生的过程，对机理函数作初步筛选；然后采用Popescu法通过函数拟合的线性相关度对机理函数作进一步的推断；最后采用单个扫描速率的微分法与等转化率法相结合的方法，以活化能为判据确定最概然机理函数。

This paper is made up of 6 chapters; chapter 1 summarizes the interaction of low molecular weight compounds with DNA; chapter 2 to 6 focus on the electrochemical studies of some organic dyes such as pyronine B, phenosafranine, brilliant cresyl blue, crystal violet, neutral red etc. and their interaction with DNA, respectively. Different electrochemical methods such as linear sweep voltammetry, cyclic voltammetry and UV-Vis spectrophotometric method are applied to the investigation.

论文由6章组成，第1章综述了小分子和DNA的相互作用；第2至6章分别筛选了吡啰红B、酚藏花红、灿烂甲酚蓝、结晶紫和中性红等小分子染料，以汞电极如静汞电极、滴汞电极作为工作电极，采用循环伏安法、线扫极谱法等电化学手段，结合紫外-可见分光光度法，研究了它们的电化学行为以及和DNA的相互作用。

An efficient protective material for metal relics was studied in the paper. Volatile rust proofing sieve, vapor inhabitability of mass loss and closed space volatile weight-loss methods were adopted to study many monocomponent volatiles corrosion inhibitors . The best VCI was chosen from them. By mixing, the two composite VCIs were made for iron and copper.

本研究通过采用气相甄别法、失重法和封闭空间挥发减量法实验筛选单组分气相缓蚀剂并进行复配实验，分别制备出铁质文物和铜器文物用的复合气相缓蚀剂，其中铁质文物复合气相缓蚀剂Ⅰ由有机胺A和乌洛脱品以质量比1：1组成，其缓蚀率为96.5％，铜器文物复合气相缓蚀剂Ⅱ由BTA和酚类W以质量比1：1制成，其缓蚀率为94.5％。

The framework established was practical and universal, and could provide abase for specific area to select its assessment indicators. The operable indicatorsystem developed which synthetically reflected the sustainable development status ofliling could fill up the blank of regional sustainable development evaluation in liling,and this work was of practical and strategic significance to a certain extent. Inallusion to the flaw existed indimensionlessness of original data in grey system,mean value method was adopted instead of traditional standardized method, and theguidance for confirming the direction of eigenvector was first brought to grey systemin the application of sustainable development evaluation aimed to increase theobjectivity and accuracy of evaluation. The evaluation results on liling could supplybeneficial references for decision makers to make and adjust sustainabledevelopment strategic goal and counter measures.

本文所构建的区域可持续发展指标体系框架具有实用性和通用性，可作为特定区域在筛选具体评价指标时的参考依据；针对醴陵市建立的区域可持续发展指标体系易于操作，能够综合反映醴陵市可持续发展的各个方面，在很大程度上填补了区域可持续发展评价研究的空白，具有一定的实践意义和战略意义；应用主成分分析法评价各子系统的可持续发展水平时，针对原始数据处理存在的弊端，提出以均值法代替传统的标准法，并首次引入了特征向量确定的原则，从理论上可提高评价的客观性与准确性；对醴陵市区域可持续发展状况的评价结果，可以为醴陵市可持续发展总体战略目标及相关措施的制定与结构调整提供有益参考。

In this study, aerobic biodegradability of 26 different kinds of dyes was tested, using static flask screening test, Warburg respirometer and semi—continuous activated sludge system; then biodegradability of 22 of the dyes under anaerobic condition was investigated by batch and continuous—flow anaerobic digesters; anaerobic metabolic substances of some dyes were analysed by several methods and the pathway of anaerobic degradation of C.

本文用静置烧瓶筛选试验法、瓦勃氏呼吸仪试验法和半连续活性污泥法对26种不同化学结构类型的染料的好氧生物降解性能进行了测定评价；对其中22种染料用间歇厌氧消化反应器和连续流厌氧反应器进行了厌氧生物降解性能的试验，采用多种仪器对部分染料的厌氧降解产物进行检测，对1种蒽醌染料的厌氧降解途径作了推测。

Cell lines was established in vitro cell culture system. Only one of it grows well during gene targeting operation. The sex of these cell lines was identified by Sry-PCR.Using lipotransfection method to transfer pZJ into pig fetal fibroblast cell. After 7 days G418 selection, 30 neo-positive cell clones were obtained, among them, 12 of which is non-green ones.

用Sry-PCR法和染色体核型分析法鉴定细胞系的性别，采用优化的脂质体转染法将pZJ转染猪胎儿成纤维细胞，经7天G418(600μg／ml)药物筛选，共获得30个neo阳性细胞克隆，其中12个为非绿色细胞克隆，仅有7个可以扩大培养。

The inconsistency of judgment matrixes, great difference of relative weights and other problems often arise during screening evaluation index by AHP. To solve these problems, a new AHP method is presented by combining the weight vector rang estimation analytic theory with traditional evaluation index rejecting method. Finally, an example of deciding a evaluation index system for submarine pipeline engineering project is given and the result proves that the method is effective and feasible.

针对用层次分析法进行评价指标筛选过程中经常出现比较矩阵不一致、相对权重值差别较大等问题，将权重向量区间估计分析理论与传统的评价指标剔除方法相结合，提出了一种新的AHP评价指标筛选方法，并以海底管道工程方案评价指标体系的确定为例进行了实际应用，结果表明该方法具有较强的实用性和可操作性。

By using the teachers'questionnaire and the Rutter child behavior meter, the study investigated large, medium and small three classes of the kindergarten in Dalian.Based on the self-making standards, the study screened five children who had serious problems, and comparing the scores of the questionnaires before and after the investigation.

主要采用教师提名法和Rutter儿童行为量表对大连市某幼儿园的大、中、小三个班的幼儿进行了儿童行为问题的调查问卷，设定筛选标准，筛选出问题较严重的5名幼儿，并对5名幼儿的行为问卷量表得分进行了前后测比较。

Mut+recombinants were selected by PCR and high yield Mut+ recombinant was picked out by double film immunoblotting.

PCR筛选出利用甲醇快速型的重组子后用双膜免疫杂交法筛选出表达量较高的重组菌株。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。