筛选法

The development of Chinese herbal medicine is one of main methods for peasants to increase income in the eastern mountain area of Liaoning.The main medicinal plants in Liaoning Province are schisandra,asarum,dioscorea, panax,gentiana,aconitum,fritillaria,lilium,astragalus,saposhnikovia,atractylodes,polygonatum, clematis,dictamnus,etc.The soils of nearly 20 species of the medicinal plant were studied in this paper.The differences among different medicinal plants were analyzed.The species and quantity of fungi,bacteria and antinomycete in soil of medicinal plants were clear.With the pathogen of main diseases of medicinal plants as target,the strains in the control of pathogen were screened from soil of medicinal plants.

在本研究中，首次以辽宁省主栽药用植物：五味子、细辛、穿山龙、移山参、龙胆、黄花乌头、平贝母、百合、黄芪、防风、苍术、玉竹、威灵鲜和白鲜皮等近20种药用植物土壤为研究对象，应用平板稀释法和磷脂脂肪酸测定法对土壤微生物多样性进行研究，通过研究明确了影响土壤微生物多样性的因素；土壤微生物与药用植物关系；以药用植物土壤微生物为供试菌株，药用植物近年来主要病害为靶标菌，进行生防菌株筛选。

This paper studied two new processes for catalytic synthesis of decabromodiphenyl ether which were carried out distinguishingly by brominating diphenyl oxide with bromine and bromine chloride in the presence of a novel metal catalyst.

本文研究了采用新型金属型催化剂催化合成十溴二苯醚的溴素法和氯化溴法两种新工艺；利用均匀实验设计和单纯形优化试验技术对两种新工艺的工艺条件进行了优化研究，分别确定了两种工艺路线的最佳工艺条件；通过筛选，确定了一种十溴二苯醚的精制新工艺。

Methods:By means of germinative test and agar diffusion,130 sorts of Chinese herbal medicine were screened synchronously.

采用琼脂扩散法和芽管试验法对 1 30种中草药进行同步筛选。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Supported solid bases and metal oxide catalysts were prepared successfully by isochoric impregnation and the co-precipitation method, and were used for synthesis of biodiesel from transesterification of rapeseed oil and methanol. The high active solid base catalysts: KF/Al2O3, ZnO/La2O3 and La2O3 were found in this paper.

本文用等体积浸渍法和共沉淀法制备了负载型和金属氧化物型固体碱催化剂，并应用于催化菜籽油与甲醇酯交换合成生物柴油的反应，筛选出了高活性的KF/Al2O3，ZnO/La2O3和La2O3三种固体碱催化剂。

Titania-supported nonprecious oxides catalysts have been prepared by impregnation and coprecipitation, respectively, and tested for carbon monoxide oxidation. The supported copper oxide catalyst showed higher catalytic activity for CO oxidation.

对浸渍法和共沉淀法制备得到的各种TiO2负载氧化物催化剂进行了活性组分的筛选，结果发现，两种方法得到的CuO催化剂均具有优良的CO氧化催化性能。

Based on phenotypical and biochemical properties as well as its 16S rRNA gene sequence, the bacterium was identified as Paenibacillus polymyxa. In vitro antifungal spectrum assay vindicate that it was active against a broad range of phytopathogens and inhibited the growth of thirteen fungal pathogens.

采用对峙培养法筛选拮抗内生细菌，根据菌株形态、生理生化特性结合16S rRNA基因序列分析鉴定菌株；采用硫酸铵沉淀法提取抗菌粗蛋白，研究其对水稻恶苗病菌菌丝生长和孢子萌发的影响。

Methods: MUC1/Y extracellular domain was used as a target molecule to biopan Ph. D. 12 phage randem peptide library. Two protocols using affinity gel and cell culture plates respectively were carried out. Positive phage clones were identified by ELISA. ssDNA sequencing was done on 16 positive phage clones to get the amino acid sequences of MUC1/Y-binding peptides. Immunohistochemistry was done to show the capacity and specificity of positive phage clones to bind the tumor cell lines.

以MUC1/Y黏蛋白的胞外段蛋白(MUC1/Yex)为靶分子，用凝胶亲和法和酶联板法分别筛选十二肽噬菌体随机肽库，ELISA鉴定阳性克隆，DNA序列测定后确定MUC1/Yex结合肽的氨基酸序列；免疫组化鉴定阳性噬菌体克隆与正常及肿瘤细胞的结合能力及特异性。

After research on the kinds of culture media, various content of hermone, ultraviolet irridation, temperature and intensity of light in the culture, the callus clone with higher content of flavonoids and ginkgolides of Ginkgo biloba spiraster screened using photometer and HPLC combined.

通过对培养基种类、多种不同浓度的激素组合、紫外线照射、培养过程的温度和光照等条件的研究，采用分光光度法和HPLC法相结合的方法筛选出了银杏黄酮和萜内酯含量都较高的银杏幼叶愈伤组织无性系。

Sodium formaldehyde sulfoxylate can break down into formaldehyde and sulfur dioxide in process of using.

吊白块在使用过程中会分解出甲醛和二氧化硫，先用乙酸铅试纸法[1]对样品进行定性筛选，可疑样品再用分光光度法[2]测量甲醛的含量，判断是否含有吊白块。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。