筛选法

To solve these problems, a new AHP method is presented by combining the weight vector rang estimation analytic theory with traditional evaluation index rejecting method.

针对用层次分析法进行评价指标筛选过程中经常出现比较矩阵不一致、相对权重值差别较大等问题，将权重向量区间估计分析理论与传统的评价指标剔除方法相结合，提出了一种新的AHP评价指标筛选方法，并以海底管道工程方案评价指标体系的确定为例进行了实际应用，结果表明该方法具有较强的实用性和可操作性。

The invention provides a method for producing transgene sugarcane quickly with pmi genes. The method primarily comprises the steps of establishing a mannitose screening system; transformation of a gene gun; acquisition of resistant callus; differentiation and screening of the resistant callus; acquisition and rootage of resistant plantlets; molecule detection of resistant plantlets regenerated and detection of pmi gene expression by the chlorophenol red detection method.

一种利用pmi基因快速获得转基因甘蔗的方法，主要内容包括：甘露糖筛选体系的建立，基因枪转化和抗性愈伤组织的获得，抗性愈伤组织的分化筛选，抗性苗的获得和生根，抗性再生苗的分子检测和氯酚红法检测法检测pmi基因表达。

Interaction between fusion peptide of influenza virus A and its related antisense peptides was studied. A peptide was screened out with high binding ability to fusion peptide and the dissociation constant was shown at the level of umol/L. In order to quantitatively characterizing the affinity interaction, a system consisted of quartz crystal microbalance biosensor and flow injection analysis was designed and established.

据此初步结果，研究了甲型流感病毒融合肽与其反义肽的相互作用；提出了基于反义肽的组合化学法筛选目的多肽或蛋白质的亲和配基的全新的研究思路，筛选到了与融合肽具有强相互作用的多肽，其与融合肽的解离常数达到umol/L。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The performance of the final model was evaluated according to the Root Mean Square Error of Prediction and the Correlation coefficient in prediction and calibration sets.

研究了利用联合区间偏最小二乘法和遗传偏最小二乘法等筛选特征谱区的方法，通过交互验证法确定偏最小二乘模型的主成分因子数和筛选区间，并以预测均方根误差和相关系数R作为模型的评价指标。

Then the recombinant plasmid transformed E.coli.JM109. One positive clone was obtained from the libraries with casein flat and ELISA sieve method.

用酪蛋白平板法和酶联免疫吸附的活性筛选方法从基因文库中筛选到一株产海洋低温碱性酶的阳性克隆（命名为pHH1）。

MethodsThe resin suitable for separation of chromone glycoside was determined by static absorption experiment on seven different kinds of resins, and further more, the absorption and elution of chromone glycoside on the selected resin were studied by fixed bed absorption experiment. ResultsThe AB-8 resin was founded to be suitable for separation and purification of chromone glycoside.

方法采用静态吸附实验考察D101，AB8，S8，X5等7种大孔吸附树脂对升麻苷和5O甲基维斯阿米醇苷两种色原酮苷的吸附效果，筛选出吸附性能较好的树脂；对所筛选出的树脂，进一步采用固定床吸附法分离玉屏风提取物中色原酮苷，探讨主要参数对色原酮苷固定床穿透曲线及洗脱曲线的影响。

A trivalent plant expression vector containing Bt cry1Ah gene, cry1Ie gene and glyphosate-tolerant 2mG2-epsps gene was constructed, glyphosate isopropylamine salt as a screening agent, 2mG2-epsps gene as a selectable maker gene. The vector was transfer into maize immature embryonic calli by microprojectile bombardment, and 69 T0 generation plants were obtained. PCR analysis showed that 17 plants had the integration of insect-resistant cry1Ah, cry1Ie gene and glyphosate-tolerance 2mG2-epsps gene.

同时构建了含有Bt cry1Ah和cry1Ie基因和耐草甘膦2mG2-epsps基因的三价植物表达载体，通过基因枪轰击法转化玉米愈伤组织，以2mG2-epsps为筛选标记基因，以草甘膦异丙胺盐作为筛选剂，获得T0代转化植株69株，PCR检测结果表明：有17株已完整的整合有抗虫基因cry1Ah、cry1Ie和耐草甘膦基因2mG2-epsps的3个目的基因，RT-PCR分析外源基因可以正确转录，已获得结实转基因植株。

Based on the screening of chlorpyrifos biodegrading fungal strain, Cladosporium cladosporioides (the ID of fungi preservation CCTCCM207111. Accession number of ITS sequence analysis Genebank EF405864), extracellular enzymes were extracted. Applying the composite rotatable design, a model of the optimal mixture protect agents was established. After the optimal composition was validated, the enzyme preparation storage experiment was tested to determine the stability of biodegradation by high performance liquid chromatography analysis.

在自主筛选毒死蜱高效降解真菌Cladosporium cladosporioides（菌种保藏号CCTCCM207111，ITS序列分析GeneBank登陆号EF405864）的基础上，发酵提取其胞外酶，采用&通用旋转回归法&筛选了酶活性保护剂的最佳配方，并进行验证；利用所得配方进行制剂贮藏实验，利用HPLC检验其降解性能稳定性。

After analyzed the situation of the major industrialproducts in area of Hubei Province it put forward some principlesand factors that influenced the choice of these products, andsummarized five parterres of development, including Defir Choiceof the major products, applying the Fuzzy comprehensive evaluationduring choosing the major products, the second sieving products,applying the Analytic Hierarchy Process during choosingproducts, and applying the Genetic Algorithms during theproducts sieving enterprises.

方法研究是本文的重点，在这一部分提出了选择重点产品的德尔菲法、模糊综合评判法、二次筛选产品法、层次分析法、遗传算法，并进行了应用分析。这些方法对产品的选择提出了不同的模型和见解，对于我国地区重点工业产品的确定将有十分重要的现实意义和指导作用。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。