筛选法

The technique path of the research is : on the rules of obtainable of data, specialty, sensitivity, representative, characteristic of full-scale and perspicuity of indexes, used external statistic way which includes ways of a coefficient of variation, case cluster and relation analysis, variable cluster and subjective Delphi, sift from initial index system, and finally get a complete evaluation index system for labor relation which meet the external technique requirement and subjective theoretic and practical analysis.

本论文研究的技术路径为：以指标资料的可获得性，指标的特异性、敏感性、代表性、全面性和简明性为原则，运用包括变异系数法、样本聚类和相关分析法、指标聚类法等客观的数理统计方法和主观的德尔菲法，对初始的指标体系进行了筛选，最终确定了满足客观技术要求和主观理论经验的完整的劳动关系评价指标体系。

To make occurrence degree prediction, single factor regression algorithms, stepwise regression algorithms, discriminatory analysis, Markovian model, fuzzy mathematics theory and Back propagation neural network algorithms are studied and models are builded.

将筛选出的主要气象因子作为预测因子，分别用单因子回归预测法、逐步回归预测法、判别分析预测法、马尔柯夫链预测法、模糊数学预测法与神经网络预测法建立了关中地区小麦吸浆虫的发生程度的预测模型。

After analyzing the data which were collected through pilot survey using methods of response rate, variation degree, correlation analysis and asking experts, items were selected and made a formal questionnaire, and then formal questionnaire survey was conducted to 398 inpatients in 6 general hospitals graded differently in Taiyuan, Shanxi from Nov.18th, 2004 to Nov.

将预调查所得数据采用应答率法，变异度法，相关分析法，以及专家意见征求法进行项目的筛选，研制成正式量表，然后又于2004 年11 月18 日-24 日对山西省太原市6 所不同等级医院的398 名住院病人进行现场问卷正式调查。

The morphological and cytopathological characters of the viruses were observed by electron microscopy The types of virus were identified with I-ELISA, DAS-ELISA, TAS-ELISA, RT-PCR and IC-PCR. The CP genes of the main viruses identified in this research were amplified by PCR, cloned into pGEM-T and sequenced. The pumpkin germplasm from Heilongjiang and Yunnan provinces was screened for resistance to the main viruses using friction and roots immersed inoculation.

应用电子显微镜负染法和超薄切片法观察病原粒体形态及细胞病理学特征；利用酶联免疫吸附测定法中的双抗体夹心ELISA、间接ELISA、三抗体夹心ELISA以及反转录聚合酶链式反应、免疫捕捉PCR方法鉴定了采集样品中的病毒种类；对被确定为南瓜主要病毒病原的外壳蛋白基因进行了PCR扩增，克隆到pGEM-T载体并进行测序；应用摩擦接种法和浸根接种法对云南、黑龙江省部分南瓜品系、品种进行了抗性筛选。

The antifungal activities of these materials were investigated and build foundation to develop low poison plant antifungal products. In experiment, polyphenols were extracted by reflux ectraction method which applyed petroleum ether to degrease and methyl alcohol solvent. Phenolic hydroxyl were examined and inspected by colour-developing reaction and the content of total polyphenols was determined by UV-spectrophotometry. Screening test were performed to the antifungal activities of polyphenol extraction by the tube dilution method to the selected ten pathogenic fungus such as Dothiorella gregaria, Rhizoctonia solani, Phyllosticta pirina, and the minimal inhibitory concentration were measured. The column chromatography and thin layer chromatography were applyed to separate and analyse cortex extracts of Dracaena cochinchinensi Lour.

实验部分采用以石油醚脱脂，甲醇为溶剂的回流提取法来提取植物多酚，用显色反应检识酚羟基；采用紫外分光光度法对多酚粗体物进行总多酚含量的测定；选取了聚生小穴壳菌、立枯丝核菌、梨叶点霉等10株常见农林业致病真菌，采用试管液基稀释法对多酚粗提取物进行了抗真菌活性筛选试验，并测定其最低抑菌浓度；采用柱层析法和薄层层析法对其中抑菌效果较好的龙血竭进行分离分析，根据其中3种化合物的光谱数据UV、IR、~(13C-NMR、1H-NMR和MS鉴定其结构。

It is a organic hexahydric weak acid. In different acidity, the dissociation degree and the color of reagent solvent were different also. Determined and draw the absorption curve of reagent in varies acidity, and figured out the dissociation constant by Perisic-Janjic method; the complex ratio of the reagent and bismuth was determinated by mole ratio method and continuous variation method; The apparent stability constant of the complex was calculated, the mechanism of dissociation and coordination were discussed.

2，6—二溴4—硝基偶氮氟胂是本实验室合成的测定铋的一个较好的试剂，对其进行了进一步的研究，在用柱层析法对其进行了提纯后，测定了试剂在不同酸华东师范大学硕：l：学位论文摘要度下的吸光度和最大吸收波长，绘制了其吸收曲线，用Perisic一Janjic法计算了试剂的六级离解常数；用摩尔比法和连续变化法测定了试剂与秘形成的配合物的络合比：计算了配合物的表观稳定常数，研究了其离解和配合机理，为筛选高效有机显色剂提供了理论依据。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

In the present study, a gradated-combinatorial screening for antitumor -active microbial strains was carried out by the gradated combination of a 96-well plate lethality bioassay using brine shrimp and a flow cytometric bioassay using mouse tsFT210 cells.

为探索如何快速获取活性菌株的筛选模式，组合使用海虾生物致死法和tsFT2 10细胞的流式细胞术筛选模型，对从青岛前海和胶州湾潮间带海泥、动植物样品中分离的 162株海洋微生物，进行了抗肿瘤活性的分级组合筛选。

The objectives of the research were to screen molecular markers of sorghum resistant gene to the physiological strain No.3 of head smut with SSR. Bulked Segregation Analysis was applied in the study. Two segregation populations, restorer line population R population 2381R/Aisi and maintain line population Tx622B/7050B, were used in the research. The amplification bands of 94 pairs primers from 109 ones were clear and stable.

为了筛选高粱丝黑穗病抗病基因的SSR标记，以高粱抗病亲本(7050B、2381R)和感病亲本（Tx622B、矮四），及其杂交组合后代为材料，用CTAB法从叶片中提取高粱基因组DNA，通过SSR技术对高粱丝黑穗病3号生理小种抗性基因进行了初步筛选，结果表明：供试109对SSR引物中筛选出扩增条带清晰且稳定的引物94对。

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如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。