查询词典 sequencing problem

Branches, leaves and phloem of apple collected from an old orchard in Yanji, Xinjiang were used for total RNA isolation specific fragments of Apple fruit crinkle viroid were amplified by RT-PCR using the primers disigned according to the sequence AB104558 on GenBank. Ten sequences were obtained through recovering, cloning and sequencing, and also registered in GenBank accession No.

采集新疆焉耆地区的老果园中的苹果树枝条、叶片和韧皮部，提取总RNA，根据GenBank中的AB104558序列设计引物，利用RT-PCR技术扩增出苹果皱果类病毒（Apple fruit crinkle viroid ，AFCVd）特异条带，通过回收、克隆和测序，结果表明，获得的10条序列，已在GenBank中登录（登录号：EU031507～EU031516）。

Long-PCR amplification, clone and primer-walking sequencing methods were employed in determine the complete sequence of mitochondrial genome of tokay.

还采用长PCR扩增等方法，测定了大壁虎线粒体基因组全序列。

Polymorphism of HLA-DQB1 promoter region in Hans IDDM patients and normal controls have been identified by PCR, PCR/SSCP and PCR/sequencing methods.No differences were found in y and s box between patients and controls carrying different allele as well as in different ethnic groups. There are two different sequences in x box,but CCTAGAGACAGATT sequence locates frequently on the haplotype with DQB1.0302 allele. Polymorphism between transcription point and y box (at position －44～-46 and －59～-61) might be associated with the genetic susceptibility to IDDM. Additionally,a new single base mutant (CACC→CAC A ) was found at position －131 and －128 in two patients carrying DQB1.0601 allele.

结果显示携带不同等位基因的患者与对照者DQB1 5'-调控区y、s box核苷酸序列相同，且与白种人基因结构一致；y box核苷酸序列存在二种结构，CCTAGAGACAGATT序列常常与DQB1.0302等位基因在同一单倍型；转录起始位点至y box间-44至-61位存在多态性，-59至-61位AAG等位基因可能与1-型糖尿病易感相关联；在2例携带DQB1.0601等位基因患者的-131至-128位间发现CACC→ACA A单个碱基取代突变。

To investigate the methods to effectively and simply assess the CAG repeat size of HD gene which was necessary for gene diagnosis of Huntington disease, the sequence including polymorphic CAG repeat of HD gene was amplified by PCR with TaKaRa LA Taq DNA polymerase and GC buffer. PCR products were analyzed on polyacrylamide gel to distinguish normal alleles from HD alleles. The DNA fragments of affected alleles were recovered from polyacrylamide gel as templets for secondary PCR. The secondary PCR products were cloned into T vector for sequencing analysis to determine CAG repeat size. A total of 20 normal individuals and 3 members from a HD pedigree were included in this study.

为了简单高效检测HD基因开放阅读框5'端n三核苷酸重复序列，建立快速准确的亨廷顿病(Huntington disease， HD)基因诊断方法，应用TaKaRa LA Taq DNA聚合酶配合GC buffer扩增HD基因包含n重复序列的目的片段，非变性聚丙烯酰胺凝胶电泳检测后回收n拷贝数异常增多的目的片段，再次PCR扩增后将产物连接至T载体，进行DNA测序确定CAG的拷贝数。

What is making the Human Microbiome Project feasible is the recent development of superfast gene sequencing technologies.

近来基因序列技术的超速发展使人类微生物组计划成为可能。

In Part Two of Chapter One, the samples of sporocarps, cultured mycelia, and root-tips collected in a 15×30 plot were studied using ITS-RFLP and ITS-sequencing.

我们应用ITS-RFLP和ITS序列比较的方法对地上子实体、地下菌根及分离培养的菌种进行了种类鉴定和分析。

Wastewater treatment performance was investigated in six sequencing batch bioreactors which were operated under different pH for the treatment of high-strength oil-containing wastewater using mixed yeast isolates.

在使用混合酵母菌菌株处理高浓度含油废水中，研究了序批式反应器内酵母菌在不同起始pH条件下对废水的处理效果。

Methods The samples from oil-containing wastewater were separated and cultured to screen oil degradation bacteria. The strain was identified through sequencing its 16S DNA and BLAST in GenBank combined with routine biochemical reactions of the bacteria.

含油脂废水中取样，通过分离、培养，筛选出以油脂为唯一碳源和能源并能分解油脂的菌株，对其基因组16S DNA测序，在核酸数据库中进行BLAST比对，并进行行生化反应，进行菌种的鉴定。

The sequence "SNYE" in signal peptide was the same as "SNYE" in result of P2 N- terminal sequencing.

在信号肽区，序列&SNYE&与蛋白测序结果中的部分序列相同。

An expressed sequence tag analysis was performed to identify major genes involved in silkworm gonad development. 16737 ESTs were generated by sequencing cDNA clones from the two libraries (8407 from testis library and 8330 from ovary library), and assembled into 2107 contigs and 3532 singlets.

利用表达序列标签方法分析参与家蚕性腺发育的基因，通过测序获得16737条ESTs（精巢8407条，卵巢8330条），拼接这些ESTs共得到2107个重叠群和3532个单拷贝。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。