查询词典 sequencing problem

Methods The total RNA was acquired from young rabbit particular cartilage, the BMP7 gene was inserted into pcDNA-3.1 to construct eukaryotic expression vector plasmid of pcDNA3.1-BMP7; the target gene-BMP7 was identified respectively by PCR analysis, restriction enounces analysis and nucleotide sequencing; the plasmid was transfected into adult rabbit knee articular chondrocytes, then the expression of BMP7 was detected by in situ hybridization, PCR and Western blotting.

从体外培养的幼兔膝关节软骨细胞中提取总RNA；按GenBank BMP7基因序列化学合成2条引物，采用RT-PCR方法得到BMP7基因；将BMP7基因片段插入到真核表达载体pcDNA 3.1中，构建pcDNA3.1-BMP7真核表达载体质粒；利用双酶切、PCR及核苷酸序列分析鉴定BMP7目的基因；将重组pcDNA3.1-BMP7真核表达载体质粒转染家兔关节软骨细胞，分别采用原位杂交、PCR、Western blotting法测定BMP-7表达。

And through questionnaires sequencing chart analysis methods extrapolated through integration organizations is the most important factor in the failure of integration.

并通过问卷排列图分析的方法，推导出组织整合失利是整个整合失败的最主要因素。

The results of sequencing was used to analyse sequence homologies in the genebank database.

结果：经动物实验证明，实验所用Hca-F和Hca-P细胞具有稳定的淋巴结转移能力。

Simultaneously introduces the domestic and foreign most advanced production equipment, and is inducts the ISO9001 international quality control system and the ISO1400 environment system and the sequencing comprehensively in the quality manages.

同时引进国内外最先进的生产设备，并且在质量是全面导入ISO9001国际质量管理体系和ISO1400环境体系和程序化管理。

Increased security concerns and critical sequencing of processes have prompted the use of locking devices and interlocks for valves.

为了加强工艺流程顺序中重要和关键部分的安全性，需要采用阀门的锁定装置和连锁装置。

Questions come in many forms, including unscrambling words, spelling challenges, multiple choice, true/false, jumbles, sequencing, matching and more.

问题来了许多形式，包括解读换句话说，拼写的挑战，选择题，真/假， jumbles ，序列分析，匹配和更多。

Methods:DNA was extracted from tissues of HCC and amplified by PCR.MI was determined by using seven microsatellite markers and mutational ana lyses of IGFⅡR by running sequencing gel electrophoresis.

用QIAamp方法提取肝细胞癌DNA，选择7对引物，经PCR扩增，走DNA序列胶电泳，测定其MI的阳性率及有无IGFⅡR基因的突变。

A set of microRNA markers, which is hard to be quantified and quantitated before, in the serum of diabetes patients was also established employing the Solexa sequencing.

这项工作一方面在运用新测序技术研究复杂疾病进行了方法学的尝试，同时，研究结果也丰富了中国人群中II型糖尿病风险基因的研究结果。

This review focuses on newly developed strategies for miRNA quantification, and elucidates in detail the probe-hybridization based methods including Northern blotting, microarray, gold nanoparticle labelling, and splinted ligation with radioactive labels. The amplification-based methods including quantitative PCR, rolling cycle amplification, invader assay, and the next generation sequencing methods were also discussed. The advantages and disadvantages of these methods were compared.

文章系统地介绍了最新发展的microRNA定量检测方法，详细阐述了基于探针杂交技术的Northern blotting法、微阵列芯片法、纳米金标记法、桥连同位素标记法，以及基于扩增技术的定量PCR检测法、滚环扩增法、引物入侵法和新一代大规模高通量测序法等，并对这些方法的优缺点进行了分析比较。

Supplanting this approach with routine sequencing of all the genes is consequently attractive and, more importantly, scalable.

因此，用对所有基因进行常规测序的方法代替当前方法很有诱惑力，并且是可以实现的。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。