查询词典 sequencing problem

After the sequence and open reading frame were confirmed by DNA sequencing, the GADes gene was expressed in E.coli. SDS-PAGE analysis suggested that the bacteria BL21 produce a kind of fusion protein of 92KD and the expressed GADes fusion protein shows expected immunoreactivity by Western Blot.

SDS-PAGE电泳和Western Blot分别鉴定融合蛋白大小和免疫活性后，对表达条件进行优化，对包涵体进行分离、溶解和复性处理，以提高可溶性融合蛋白的产量，使融合蛋白达总蛋白的50％，相当于1.495g/每升培养物。

All 21 exons with flanking intronic sequences, as well as a 263 bp fragment of the 5" flanking region, of GARS-AIRS-GART gene, were screened in the same five populations, by the method of PCR-SSCP analysis, combined with sequencing reactions. We detected 10 SNPs, C-179T from 5" flanking region, A5669G form exon 3, C6545Afrom exon 4, A7777G from intron 4, G10854T, C10867T, G10898T from intron 6, A16197G from intron 11, C21228T from intron 14, and C29686T from exon 21. These ten polymorphic sites were nominated as GARS1, GARS2, GARS3, GARS4, GARS5, GARS6, GARS7, GARS8, GARS9, and GARS 10, respectively. The mutation of C D A at GARS3 was a non-synonymous mutation, wh

其中外显子4中的6545处的c~A突变导致对应的氨基酸序列中的第173位氨基酸发生变异：天冬氨酸一谷氨酸；10个SNP位点依次命名为：GARSI、GARSZ、GARS3、GARS4、GARSS、GARS6、GARS7、GARSS、GARSg、GARS 10；(2)10个SN'P位点在不同的鸡种中的基因型分布差异显著(P.05)，进一步的方差分析显示，GASRI位点以及GARS3位点与肌肉肌昔酸含量之间存在着显著的关联；(3)在所检测的5个群体中GARsl位

The results of DGGE profile showed that the diversity of bacterial decreased with the fermentation process and Lactobacillus manihotivorans became the dominant species at the end of the fermentation. According to the results of sequencing and blasting of clones, only Weissella, Bacillus, Acetobacter and Lactobacillus were detected in two cloning libraries. And some genuses were found and not identified in the previous researches of liquor Daqu and fermented grains.

DGGE图谱分析显示，随着发酵时间的延长，酒醅中的微生物多样性不断降低；发酵结束时Lactobacillus manihotivorans成为最主要的优势菌种。16S rRNA克隆文库的测序结果在数据库中进行比对后表明，浓香型和芝麻香型酒醅中的微生物种属存在巨大差异，仅有Weissella、Bacillus、Acetobacter和Lactobacillus 4个属的微生物在两个文库中均能检测到，发现并鉴定出在白酒曲药和酒醅的研究中尚未报道的细菌属。

Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

Four B-hordein genes, designated BH1-BH4, were cloned using PCR amplification from two hull-less barley cultivars,ZQ7239 and ZQ148, collected from Tibet. The results of sequencing indicated that BH1-BH4 contained complete open reading frames.

从两份西藏青稞材料中分离克隆出4个B组醇溶蛋白基因(BH1-BH4)，DNA测序结果表明：它们均包含完整的开放阅读框。

Two novel SNPs were found in the fifth intron of Megsin gene by direct sequencing.

在第5内含子发现两个新的SNP位点。

Methods The hairpin sequences of siRNAs targeting VR1 gene of rat were designed, and two pairs of oligonucleotide sequence were synthesized. The annealed oligonucleotide fragments were cloned into linearized pRNAT-U6.2/Lenti expression vector and identified by PCR and DNA sequencing.

设计靶向大鼠VR1基因的发夹状siRNA，合成两对互补的寡核苷酸序列，退火后克隆到酶切的pRNAT-U6.2/Lenti siRNA载体，通过PCR和DNA测序鉴定重组质粒。

Results DNA sequencing showed that the oligonucleotide fragments were correctly cloned into linearized pRNAT-U6.2/Lenti expression vector and the expression of VR1 mRNA in L4-L6 DRG neurons was inhibited significantly by pRNAT-U6.2/Lenti-siVR1 after intrathecal injection in rats.

结果 测序鉴定证实目的寡核苷酸片段被克隆到pRNAT-U6.2/Lenti载体中；经蛛网膜下腔注射后，pRNAT-U6.2/Lenti-siVR1可下调正常大鼠L4-L6DRG神经元内VR1mRNA的表达。

Great, realistic, Scrabble-style action Letter bag, personal tray, password protected Automatic turn sequencing and email updates Unlimited simultaneous games Online rule book Winner announcements Administrative controls such as game deletion, score adjustment, and more.

很强的现实针对性，拼字式行动的信袋，个人进纸匣，密码保护，自动转测序和电子邮件更新无限的同时线上游戏规则书赢家公布的行政管制，例如游戏删除，分数调整，更多。

Using the total RNA of merozoites of Eimeria acervulina isolated from Guangdong Pro- vince as template,a partial segment of 3-1E gene was amplified by RT-PCR. The gene fragment 682 bp inlength was cloned into pGEM-T Easy vector,and the recombinant plasmid was identified by PCR,restric-tion enzyme analysis and sequencing. The homology analysis revealed that the nucleotide homology be-tween the 3-1E gene of the E.

根据GenBank中登录的堆型艾美球虫3-1E基因序列，设计了3条引物，以广东株堆型艾美球虫裂殖子总RNA为模板，利用反转录-聚合酶链反应扩增获得了3-1E基因部分片段，将这一片段克隆至pGEM-T Easy载体中，经PCR、限制性内切酶分析和克隆片段的序列测定、比较，证实了克隆片段的可靠性。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。