查询词典 sequencing problem

Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection. A signal significantly greater than background binding was observed from the third to the fifth round of selection on CHOEGFRGFP1 and CHOK1 cell, but a part of polyclonal scFv bound EGFR specially. About 45% of the selected clones contained a fullsized insert of 1 kb. One unique human antiEGFR scFv (F4scFv) was isolated by analyzing with cell ELISA and DNA sequencing.

结果 经过5轮筛选，细胞裂解液中洗脱出噬菌体效价有500倍以上增长；细胞ELISA结果显示多克隆pscFv与CHOEGFRGFP1细胞和CHOK1细胞均有明显结合，但有部分特异性结合EGFR；菌落PCR显示约45％克隆中含有完整的1kb scFv片断；经细胞ELISA、 DNA测序检测共获得1株EGFR特异性单链抗体，命名为F4scFv。

Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Fifty-nine insect species have been included in genome sequencing projects, whole-genome sequences of six insect species, i.e., Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Apis mellifera, Aedes aegypti and Tribolium castaneum, have been reported.

目前有59种昆虫已经列入基因组测序计划，其中6种昆虫（黑腹果蝇Drosophila melanogaster、冈比亚按蚊Anopheles gambiae、家蚕Bombyx mori、意大利蜜蜂Apis mellifera、埃及伊蚊Aedes aegypti和赤拟谷盗Tribolium castaneum）的全基因组序列已经报道。

One positive clone was obtained from the libraries with the tributyrin flat and ELISA sieve method. Analyzed the sequencing result, the positive clone include the Open Read Frame of low-temperature alkaline lipase gene.

序列测定分析表明，此重组质粒包含有长度为此95lbp的低温脂肪酶基因的完整的开放读码框架和上游调控序列。

Results The mecA gene and tst gene were detected,and were made the gene sequencing successfully.

结果 成功的对金黄葡萄球菌mecA基因和tst基因进行了检测，并进行基因测序。

The best bets for ultrarapid, low-cost sequencing are single-molecule approaches.

对于超高速、低花费的测序最好的办法是单分子的方式。

The effect of excess sludge reduction by uncoupler of 2,4-dechlorophenol was studied using Sequencing Batch Reactor.

采用序批式活性污泥反应器试验研究了解偶联剂2，4-二氯酚的污泥减量化效果。

This research was on sequencing batch reactor activated sludge system, using 2,6-dichlorophenol as uncoupler with the concentration of 20,using Cu~(2+) with the concentration of 1,adding into the SBR activated sludge system.

采用间歇式活性污泥培养装置，以2，6-二氯苯酚作为解偶联剂，浓度为20 mg/L，Cu2+浓度为1 mg/L，加入到间歇式活性污泥反应器中。

The unfused human interferon aD expressed by pBV867 was confirmed by SDS-PAGE,activity assay and N terminal sequencing.Results suggested that E.Coli is able to process the signal sequence of human interferon αD.

发现&翻译联结&方式起始的基因表达水平比一般ATG起始方式高约10倍；证明大肠杆菌能够识别并加工人αD型干扰素信号多肽。

To get more factors associated with erythropoiesis in K562 cells, modified differential display reverse transcription polymerase chain reaction method was applied to identify genes with differential expression in uninduced and hemin-induced K562 cells. Remarkable differential ESTs were firstly selected for cloning, sequencing and analyzed with bioinformatics methods.

为了进一步获得分化过程中相关基因表达的信息，我们应用改良的差异显示RT-PCR技术，分析K562细胞在Hemin诱导的红系分化前后的基因表达变化，获得红系分化相关基因的线索，进而确定在红系分化中起重要作用的基因，并研究它们的作用机制。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。