查询词典 sequencing problem

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白，主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运，并维持一定细胞内外的离子梯度，我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析，发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg，Fc。

Even with the latest DNA sequencing technology, the researchers had to design a special strategy to search for the vanishingly rare mutations.

~* H/ R# l8 L' H 即便是使用最先进的DNA测序技术，研究者们还是要设计专门的策略来寻找正在消失的稀少突变。

Prana Vayu, loosely translated as the "Breath of Life", is a style of power yoga that uses sequencing to make difficult postures easier.

Prana Vayu的基本含义是&生命之气息&。帕纳瓦优瑜伽是利用顺序来使困难的体式变得容易的一个力量瑜伽风格。

Although several countries like Brazil and Mainland China are also working on sequencing the genome of Xanthomonas campestris , the overall project will instead focus on studying its structural and functional genomics .

虽然其它国如巴西及中国大陆也在做此一菌种的基因定序，但是本计画的主要目标系在研究此一菌种的结构及功能基因体上，所以基本上并没有冲突的地方。

And human and mice, tropical Xenopus and mammals, many of the same gene sequencing.

和人类以及小鼠相比，热带爪蟾的很多基因排列和哺乳动物一样。

With the completion and initiation of Oryza sativa and Zea mays genome sequencing respectively,such functions will be more obvious.

随着水稻、玉米基因组序列的完成与启动，这方面的作用将更加显著。

Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

DNA序列测定表明，克隆得到的ChIL-18 cDNA与国外报道的完全一致，包括终止密码子在内其编码区的长度为510bp，编码169个氨基酸残基的蛋白质。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18，mChIL-18)的cDNA基因序列，设计一对特异性引物，应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因，对扩增片段进行T-A克隆，经PCR、酶切鉴定及测序验证，成功获得ChIL-18成熟蛋白完整基因的克隆。

Southern blot analysis and partial sequencing showed that the candidate BAC clones actually contained pufferfish homologue of

作者相信，本河豚鱼基因组BAC文库定会在河豚鱼基因组研究和比较基因组学的研究中发挥重要作用。

However, with the advent of new sequencing technologies, genomics research is now at the threshold of a second revolution.

但是，随着新的测序技术的到来，基因组学研究正处在第二次革命的开始。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。