查询词典 sequencing problem

Mouse genotype and C.rat genotype respectively,by RFLP and gene sequencing technique.The extracting effect of the imported commercial DNA extraction kit was the best,and the copies of COWP gene were(6.450-9.861 )×10~6 in the extraction.

Real-time PCR技术测定不同浓度NTZ和GAs作用后COWP基因拷贝数，透射电镜观察NTZ和GAs对虫体生长的影响。

Sequencing results showed one displayed peptide, named A1 , and PMB (mo-K'TK'-cyclo-) had some similar amino acid combination .

测序结果显示，其中一条肽A1和多粘菌素B(mo-K'TK'-cyclo-)的部分氨基酸组合相似。

A recombinant pl asmid (designated as p8.0) was obtaining by ligating these three fragments. The p8.0 was subcloned into a plasmid (designated as p8.2) containing entire LTR of EIAV DLA. The complete nucleotide sequence of DLA strain of EIAV was determined by sequencing the p 8.2 . The purified p8.2 was used to transfect donkey leuko cyte cultures.

将此8.0kb EIAV全基因再亚克隆到含有一完整EIAV DLA株长末端重复序列的质粒中，获得一含有EIAV驴白细胞弱毒前病毒全基因的重组质粒，将其命名为p8.2，经核苷酸序列分析，证明p8.2含有EIAV前病毒的全基因。

The construction of viruses were identified by enzyme digestions, PCR amplification, sequencing, light and electronic microscope analysis.

采用同源重组的方法分别构建了带BMP—2基因和报告基因LacZ的复制缺陷型重组腺病毒表达载体，通过酶切、PCR扩增、序列测定和光镜、电镜观察等方法证实重组病毒构建正确。

Results The bcl-2 gene identified by the colony PCR was further confimed by PCR with plasmid DNA and digestions as well as DNA sequencing.

结果在筛选的阳性克隆中扩增到大鼠Bcl-2基因，经菌落PCR、酶切鉴定及DNA序列分析证实该序列正确。

Extracting exonic sequences directly from genomic sequence is difficult, but ESTs have provided a convenient means of accessing them. An expressed sequence tag is part of a gene that results from sequencing a portion of a cDNA clone that was generated from an mRNA.

从mRNA来源的cDNA文库中随机取出一个克隆，从5'末端或3'末端对插入的cDNA片段进行单向测序，所获得EST为我们研究基因表达提供了方便快捷的手段。

For ADRBK2 gene, systematically mutation screening was performed in all 21 exonic and flanking intronic regions through direct sequencing in 48 schizophrenia probands (including 16 Japanese and 32 Chinese probands) randomly selected from our subjects, and the detected variants and eight SNPs reported in the JSNP database were genotyped in 64 trios.

对于ADRBK2基因，在48名先证者(16名日本人和32名中国人)中进行突变筛查，对ADRBK2的21个外显子的所有序列及外显子与内含子邻接区域的部分内含子进行了测序；同时对突变筛查中发现的错义突变及JSNP数据库中的有关SNP，对64个核心家庭(16个日本人核心家庭，48个中国人核心家庭)的成员进行了基因分型。

For ADRBK2 gene, systematically mutation screening was performed in all 21 exonic and flanking intronic regions through direct sequencing in 48 schizophrenia probands (including 16 Japanese and 32 Chinese probands) randomly selected from our subjects, and the detected variants and eight SNPs reported in the JSNP database were genotyped in 64 trios.

对于ADRBK2基因，在48名先证者(16名日本人和 32名中国人)中进行突变筛查，对ADRBK2的21个外显子的所有序列及外显子与内含子邻接区域的部分内含子进行了测序；同时对突变筛查中发现的错义突变及JSNP数据库中的有关SNP，对64个杨L家庭(16个日本人核心家庭，48个中国人核心家庭)的成员进行了基因分型。

Both strands of exons 2, 3 and 4 of chosen colonies were sequencing.

应用PCR-SSP方法证实测序所发现的突变。

Evaluating the threat of air targets and sequencing scientifically are important to air defense command, primary reference for air defense commander distributing fire and also one of the functions of automatic command and control systems.

对空中目标的威胁程度进行科学的评估与排序是防空指挥的重要环节，是防空指挥员进行火力分配的主要依据，也是防空指挥自动化系统的重要功能之一。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。