查询词典 sequencing problem

Methods: HPV 16/18 infection was detected by nested-polymerase chain reaction, the p53 mutation was detected by direct sequencing, and the p53 and the HPV 16/18 E6 proteins were studied using immunohistochemistry on 129 pterygial specimens and 20 normal conjunctivas.

本研究利用巢叠式聚合脢锁反应分析自129例罹患翳状赘肉之翳状赘肉组织及20例正常无翳状赘肉的结膜组织检体中，是否有人类乳突病毒16及18型的感染，并利用直接定序的方式检测p53基因是否发生突变，此外亦以免疫组织化学染色法侦测p53蛋白与人类乳突病毒E6蛋白。

Methods: MUC1/Y extracellular domain was used as a target molecule to biopan Ph. D. 12 phage randem peptide library. Two protocols using affinity gel and cell culture plates respectively were carried out. Positive phage clones were identified by ELISA. ssDNA sequencing was done on 16 positive phage clones to get the amino acid sequences of MUC1/Y-binding peptides. Immunohistochemistry was done to show the capacity and specificity of positive phage clones to bind the tumor cell lines.

以MUC1/Y黏蛋白的胞外段蛋白(MUC1/Yex)为靶分子，用凝胶亲和法和酶联板法分别筛选十二肽噬菌体随机肽库，ELISA鉴定阳性克隆，DNA序列测定后确定MUC1/Yex结合肽的氨基酸序列；免疫组化鉴定阳性噬菌体克隆与正常及肿瘤细胞的结合能力及特异性。

After the recombinant plasmid was certified by DNA sequencing,the conservative region of Ki67 gene was inserted into pEGFP vector reversedly.

方法用基因重组方法将人Ki67基因cDNA保守序列反向克隆到真核表达质粒pEGFP-C1中，构建人反义Ki67核酸载体。

To deepen the results, researchers from Rhodococcus erythropolis LSSE8-1 and Gordon's LSSEJ-1 strain in the successful expansion of desulfurization have been related to the gene fragments, and the sequencing is being carried out desulfurization projects microbial bacteria work to build .

为深化成果，研究人员从红平红球菌LSSE8－1和戈登氏菌LSSEJ－1中成功扩增得到了脱硫相关基因的片断，并进行了序列测定，目前正展开微生物脱硫工程菌的构建工作。

By randomly single-pass sequencing from 5'-end of muscle cDNA library and liver cDNA library from Silkie Fowl, 16600 ESTs of muscle tissue which are more than 100bp and uncontamination and 17733 ESTs of liver tissue which are more than 100bp and uncontamination have been obtained.

利用大规模DNA序列测定方法，对丝毛乌骨鸡胸肌组织和肝脏组织的2个cDNA文库在5'端进行了大规模的单向单次的随机测序，得到了肌肉组织的16600条大于100bp高质量无污染的ESTs和肝脏组织的17733条大于100bp高质量无污染的ESTs。

It has also become a good model system to study cross-talk of different plant hormones with the development of genetics and whole tomato genome sequencing from International Solanaceae Genomics Project.Jasmonic acidis a fatty acid-derived signal and exists in the plant kingdom extensively.

番茄是最重要的蔬菜作物之一，随着番茄遗传学研究和国际茄科基因组计划(International Solanaceae Genomics Project，SOL)的发展，番茄已成为研究植物激素相互作用的理想模式植物。

A 3.12 kb region spaning from exon 13 to exon 15 in the bcr region were screened by DNA pooling and denaturing high performance liquid chromatography, and the results were varified by sequencing.

利用DNA库结合变性高效液相色谱对长度为3.12kb的bcr基因断裂丛集区进行序列变异的筛查分析，并通过测序对筛查结果进行了验证。

ITS2(Intemal Transcribed Spacer 2) region in ribosome DNA has a character of conservative that makes a low aberrance ratio, on the other side, ITS-2 region evolves itself. According to the characters, ITS-2 region sequencing is an effective parameter in taxology and pests-control..

核糖体基因的第二内转录间隔区有较高的保守特性，同时进化速度又较快，因此序列进行测序得出的种内差异，是系统发育学的有力参数，也有助于异色瓢虫分类学和农林虫害的防治。

The termini of PCR products and the misincorporation in routine PCR were studied by using UT-PCR, cloning and sequencing.

应用UT-PCR扩增、克隆等对PCR产物末端研究结果表明，PCR产物末端组成是复杂多样的，但主要是3'端突出一个A，占末端总数的67.3％；其次是平端，占末端总数的26.9％。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。