查询词典 sequencing problem

Nitrosification and denitrification in an artificial domestic wastewater with low COD were studied in a sequencing batch moving bed biofilm reactor.

在序批式移动床生物膜反应器内，对进水COD较低的条件下，模拟生活污水的亚硝化及脱氮性能进行了研究。

To investigate distribution of gene mutations in LPL exon 9 in Chinese population and to study the possible effects of the mutation on plasma lipids, variants were screened by PCR-SSCP and DNA sequencing. Ser~(447)→stop polymorphism was identified by PCR-RFLP . the plasma lipids in stop447 carrier group and stop447 noncarrier group were compared and analysis.

为探讨中国人群LPL基因外显子9常见突变的种类、频率以及其可能产生的影响，我们利用聚合酶链反应-单链构象多态性分析和DNA测序技术及PCR-RFLP技术检测出我国人群的LPL外显子9基因突变、基因型和等位基因频率，确定了中国人群Ser~(447)→stop这一多态性位点。

Methods Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用套式聚合酶链反应方法对5例艾滋病患者血浆HIV-1 env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及核苷酸序列进行分析，并计算其核苷酸同源替换率与非同源替换率的比值。

Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用巢式PCR方法对5例艾滋病患者血浆HIV-1env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及氨基酸序列进行分析，并计算其氨基酸同源替换率与非同源替换率的比值。

In chapter 1 ,the article introduces background、significance of the researching and the recent status of de novo sequencing.

本课题的焦点主要集中在图谱的预处理和图谱的从头解析算法。

The concepts behind a number of de novo sequencing methods are discussed.

讨论了很多从头测序方法背后的概念。

"Sequencing the codes for juvenile diabetes," Lara said numbly.

/ 为少年的糖尿病序列密码，劳拉失去知觉说。

After being digested with EcoR I and Xho I, they were inserted to the vectors pGEX-4T-l orientally, and then transformed into E.coli BL21(DE3) cells. The monoclone were selected and identified by means of restriction analysis, PCR and sequencing. As the result, the two recombinant plasmids with the different DNA fragements of the PrP gene were constructed and designated as pMY01[Ov rPrP(23-244)] and pMY02[Ov rPrP(91 -244)], respectively.

挑选单个菌落，提取重组质粒，经酶切、PCR扩增后琼脂糖凝胶电泳分析和测序鉴定，成功地构建了2种绵羊重组朊蛋白的表达质粒pMY01[OvrPrP(23～244)]和pMYO2[Ov rPrP(91～244)]。

Methosd The complete nuclear ribosomal DNA second internal transcribed spacer (ITS2) gene sequence of eggs in sputum from a paragonimiasis patient was obtained by directly auto-sequencing its PCR product .

先从并殖吸虫病患者痰中分离出虫卵，然后PCR扩增出虫卵中完整的核糖体DNA第二间隔区基因(ITS2 )，并直接用于测序从而获得该基因的核苷酸序列。

The results of sequencing was contrasted by BLAST software. The sequence of the PCR products shared 100% homology with Pasteurella multocida reference strain.

设计1对引物进行PCR扩增、克隆之后，送往大连宝生物公司测序，测序结果运用BLAST软件比对，与多杀性巴氏杆菌同源性为100%。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。